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Swine fibrin glue powder inhalation and preparation method and application thereof

A technology of fibrin glue and fibrin, applied in the field of biomedicine, can solve the problems such as hindering the application of fibrin sealant, difficult to control the configuration time, prolonging the waiting time for surgery, etc., and achieves the effects of optimal sealing, stable medicine, and convenient carrying and storage.

Active Publication Date: 2010-11-24
GUANGZHOU BIOSEAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, pig blood resources are abundant, and the gene sequence of pigs is very similar to that of humans, and the risk of transmission of infectious diseases carried by humans is extremely small, which greatly reduces the cost and makes the products have strong market competitiveness; however, the current Fibrin sealant is a liquid spraying agent, which is generally composed of four components: main glue lyophilized powder, catalyst lyophilized powder, main glue solution and catalyst solution. Before use, it needs to be dissolved with main glue solution and catalyst The main gel freeze-dried powder and the catalyst freeze-dried powder are dissolved separately, and then they are mixed and used after the dissolution is complete. The dissolution takes a long time, the operation is complicated, the configuration time is not easy to control, and it is easy to prolong the waiting time of the operation; especially These deficiencies seriously hamper the application of fibrin sealants in field work trauma, field surgery

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  • Swine fibrin glue powder inhalation and preparation method and application thereof
  • Swine fibrin glue powder inhalation and preparation method and application thereof
  • Swine fibrin glue powder inhalation and preparation method and application thereof

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preparation example Construction

[0034] The preparation method of the above-mentioned porcine source fibrin gel powder spray comprises the following steps: (1) preparing porcine source fibrinogen gum freeze-dried powder, porcine source thrombin freeze-dried powder, sodium chloride, sodium acetate, calcium chloride , trishydroxymethylaminomethane, physiologically acceptable antistatic agent, and weighed according to formula; Grinding, the particle size of the pulverized powder is 75 μm to 180 μm; (3) mixing sodium chloride and / or sodium acetate, calcium chloride and / or trishydroxymethylaminomethane, and a physiologically acceptable antistatic agent, Then adopt the jet milling method to pulverize, and the particle size of the pulverized mixture is 90 μm to 250 μm; (4) mix the above pulverized components in a fluidized state mixing mode to form porcine-derived fibrin glue micropowder; (5) Filled in the powder mist injection device.

[0035] Commonly used micronization methods include jet milling, ball milling, ...

Embodiment 1

[0038] 1. Preparation of pig-derived fibrinogen gum freeze-dried powder: collect 55600ml of pig blood, add 6000ml of anticoagulant containing 0.147mol / L sodium citrate and 0.154mol / L sodium chloride, and store in a low-temperature storage at 4°C Let it stand for 6 hours, let it separate into layers, and separate the upper layer of plasma; centrifuge the upper layer of plasma in a high-speed centrifuge at 10°C at a speed of 3500 rpm for 40 minutes, collect the plasma, and filter it with a 0.22 μm filter membrane; 20000ml of plasma, add 73.5g of glycine to dissolve it, cool the plasma to 4°C, add 2605.4g of sodium acetate to dissolve it, stir for 30 minutes, centrifuge at 3500 rpm for 40 minutes in a high-speed refrigerated centrifuge at 10°C, The precipitate was collected to obtain 2520.8 g of precipitate. Crush the collected precipitate, add 33 liters of buffer solution with a pH value of 7.5 made of sodium citrate and trishydroxymethylaminomethane, stir until completely disso...

Embodiment 2

[0044]1. Pig-derived fibrinogen gum freeze-dried powder: Collect 60,000ml of pig blood, add 6,000ml of anticoagulant containing 0.145mol / L sodium citrate and 0.151mol / L sodium chloride, and place it in a low-temperature storage at 5°C After 6 hours, let it separate into layers, separate the upper layer of plasma, centrifuge at 10°C at 5,000 rpm for 60 minutes with a high-speed refrigerated centrifuge, collect the plasma, and filter it with a 0.22 μm filter membrane; take 22,000 ml of filtered plasma, add Glycine 95.8g was dissolved, the plasma was cooled to 3°C, sodium acetate 2931.8g was added to dissolve it, stirred for 30 minutes, and the high-speed refrigerated centrifuge was centrifuged at 6000 rpm for 30 minutes at 10°C to collect the precipitate. A precipitate of 2685.8 g was obtained. Crush the collected precipitate, add 35 liters of buffer solution with a pH value of 7.8 made of sodium citrate and trishydroxymethylaminomethane, stir until completely dissolved, filter,...

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Abstract

The invention relates to a swine fibrin glue powder inhalation and a preparation method and application thereof. The swine fibrin glue powder inhalation comprises swine fibrin original gum lyophilized powder, swine thrombin lyophilized powder, sodium chloride and / or sodium acetate, calcium chloride and / or tromethamine and a physiologically acceptable antistatic agent. The preparation method comprises the following steps of: grinding the components into powder with suitable particle size, then uniformly mixing the powder and filling the mixture. The product is stable and convenient to carry and store and can be directly used without preparation. The swine fibrin glue preparation is sprayed on a wound surface in a powder shape, saves time for operation and wound packing, brings great convenience, has a simple production process and is safe and environmentally-friendly.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a bio-adhesive pharmaceutical composition that can rapidly form a high-efficiency bio-adhesive film and has the functions of high hemostasis, adhesion and wound closure, and prevention of inter-tissue adhesion, and is a biological hemostatic material. . Background technique [0002] In modern surgical operations, wound hemostasis and healing is a problem that needs to be effectively solved. Traditional methods generally use ligation, suture and electrocoagulation for hemostasis. Traditional suturing can easily lead to needle eye infection and leakage, and tearing of brittle tissue can easily cause tissue damage; electrocoagulation will increase tissue damage and cause tissue necrosis. Therefore, scientists are devoted to finding a new type of hemostatic material that can not only quickly and effectively stop bleeding, but also promote tissue healing. Fibrin sealant (FS) came into bei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/38A61L15/32A61L15/42C07K14/745A61K38/48A61K9/12A61P7/04A61K38/36A61K35/14
Inventor 李吉来黄星江彩霞李兆勇潘小宁
Owner GUANGZHOU BIOSEAL BIOTECH
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