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Klebsiella pneumoniae (Mannose-sensitive hemagglutination) pilus strain

A Klebsiella, mannose technology, applied in bacteria, antibacterial drugs, allergic diseases, etc., can solve problems such as no medical application value

Active Publication Date: 2010-11-10
BEIJING WONDER BIOPHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The Klebsiella pneumoniae recorded in the prior art is a pathogenic bacterium and has no medical application value; there is no report that Klebsiella pneumoniae is used to prepare a vaccine at present

Method used

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  • Klebsiella pneumoniae (Mannose-sensitive hemagglutination) pilus strain
  • Klebsiella pneumoniae (Mannose-sensitive hemagglutination) pilus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Source and identification of Klebsiella pneumoniae mannose-sensitive hemagglutination strains

[0059] The inventors established a mannose-sensitive hemagglutination fimbriae strain of Pseudomonas aeruginosa by comparing 6 strains of Klebsiella pneumoniae isolated from clinical specimens, reference (Muxia, Pseudomonas aeruginosa, Acta Microbiology, 26(2): 176-179 , 1986), screened and bred to obtain a Klebsiella pneumoniae mannose-sensitive hemagglutination strain.

[0060] The main identification features are as follows:

[0061] Fermentation of lactose on differential medium to express colored colonies;

[0062] On solid medium, it is a gray-white sticky peptone-like colony;

[0063] After several days in the broth, the liquid is noticeably viscous;

[0064] Gram negative.

[0065] Electron micrographs were performed on this strain, such as figure 1 shown, showing the presence of periphytic fimbriae. Mannose-sensitive hemagglutination and hemagglutina...

Embodiment 2

[0066] Example 2. Strain culture and vaccine preparation

[0067] The Klebsiella pneumoniae mannose-sensitive hemagglutination fimbriae strain of the present invention is cultured on a bacterial plate at 37° C. for 18 hours, the culture is collected, sterilized with a formaldehyde solution, washed repeatedly to prepare a dead bacterin, diluted with Bacterial saline was added with 1% benzyl alcohol as a preservative to prepare a bacterin.

Embodiment 3

[0068] Example 3. Animal experiment of bacterin

[0069] Use bacterial plate culture, kill bacteria with 1% formaldehyde physiological saline, wash 5 times with sterile physiological saline, detect the concentration of bacterial liquid by spectrophotometer, and then check for viable bacteria, dilute to 1.8 to 2 billion / ml, and make bacteria Seedling.

[0070] The experimental animals used in the animal experiments were 18-20 g mice. Subcutaneous injection 3 times, each injection of 0.3ml. The interval between injections is one week. On the 7th day after the last injection, the cross-agglutinated MSHA antibody titer of the immunized mice was detected.

[0071] Table 1. Detection of cross-agglutination titers of antibodies in blood of mice and MSHA antigens of two bacteria after immunization with the "bacterial" of the present invention

[0072]

[0073] A total of 43 mice were immunized successively in the immune protection test. After 17 days of automatic immunization,...

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Abstract

The invention relates to a klebsiella pneumoniae mannose-sensitive hemagglutination pilus strain which is characterized by comprising mannose-sensitive hemagglutination pilus. The invention also relates to the cultivation, treatment, application and using method of the klebsiella pneumoniae mannose-sensitive hemagglutination pilus strain. The invention also relates to a medical product prepared by the klebsiella pneumoniae mannose-sensitive hemagglutination pilus strain.

Description

technical field [0001] The present invention relates to a novel microbial strain. Specifically, the present invention relates to a Klebsiella pneumoniae mannose-sensitive hemagglutination pili strain. The invention also relates to the cultivation, treatment, use and methods of use of this Klebsiella pneumoniae strain. The invention also relates to a medical product prepared by using the Klebsiella pneumoniae strain. Background technique [0002] Klebsiella (Klebsiella) is currently the most important conditional pathogenic bacteria except Escherichia coli, and its biochemical reactions are very similar to those of Serratia, Enterobacter, and Hafnia. Some amino acid decarboxylase tests have help in identification. [0003] The genus Klebsiella can be divided into five species, namely Klebsiella pneumoniae (K. pneumoniae), Klebsiella oxytoca (R. ornithinolytica), K. planticola and K. terrigena. Among them, Klebsiella pneumoniae can be divided into three subspecies: subsp....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/108A61P31/04A61P37/02A61P35/00C12R1/22
Inventor 牟希亚郭雁群牟心赤朱洁
Owner BEIJING WONDER BIOPHARMACEUTICAL CO LTD
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