Preparation method and application of cortex meliae effective ingredients

A technology of effective components and neem bark, applied in the field of traditional Chinese medicine extracts, can solve problems such as side reactions, involving normal cells, etc., and achieve the effects of stable quality and simple method.

Inactive Publication Date: 2010-10-27
ZHEJIANG UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of cortex meliae effective ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The preparation of embodiment one neem bark effective component

[0012] Add 1500ml of ethyl acetate and ethanol (1:0.8) (volume ratio) after pulverizing 200g of neem bark medicinal material, heat and extract once to obtain an extract, concentrate the extract into an extract, separate it with a reversed-phase silica gel column, First use 20% (volume ratio) ethanol solution as mobile phase to obtain eluent I, discard it, then replace 90% (volume ratio) ethanol solution as mobile phase to obtain eluent II, and concentrate and dry eluent II Obtained sample; use preparative liquid chromatography to continue to separate the obtained sample, the separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, gradient elution. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% acetonitrile solution; at 4 minutes, the mobile phase was 80...

Embodiment 2

[0013] The preparation of embodiment two neem bark effective components

[0014] Add ethyl acetate and ethanol (1:1.2) (volume ratio) 5000ml after pulverizing 500g of neem bark medical material, heat and extract 3 times to obtain extract, extract is concentrated into extract, and it is separated with reversed-phase silica gel column, First use 40% (volume ratio) ethanol solution as mobile phase to obtain eluent I, discard it, then replace 100% (volume ratio) ethanol solution as mobile phase to obtain eluent II, and concentrate and dry eluent II Obtained sample; use preparative liquid chromatography to continue to separate the obtained sample, the separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, gradient elution. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% acetonitrile solution; at 4 minutes, the mobile phase was 80...

Embodiment 3

[0015] The preparation of embodiment three neem bark effective components

[0016] Add 2000ml of ethyl acetate and ethanol (1:1) (volume ratio) after pulverizing 250g of neem bark medical material, heat extraction 2 times to obtain extract, extract is concentrated into extractum, and it is separated with reversed-phase silica gel column, First use 30% (volume ratio) ethanol solution as mobile phase to obtain eluent I, discard it, then replace 95% (volume ratio) ethanol solution as mobile phase to obtain eluent II, and concentrate and dry eluent II Obtained sample; use preparative liquid chromatography to continue to separate the obtained sample, the separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, gradient elution. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% acetonitrile solution; at 4 minutes, the mobile phase was...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a preparation method of cortex meliae effective ingredients. The effective ingredients are obtained through the following steps: carrying out heating extraction, concentration, silicon gel post separation and elution on medical materials; concentrating and drying an elution solution; then, using the prepared liquid chromatogram for continuous separation; collecting the solution; and concentrating and drying the solution. The cortex meliae effective ingredients of the invention can be used as active components, and pharmaceutically acceptable medicine excipients or carriers are added into the effective ingredients to be prepared into preparations according to a method recorded on the pharmacy. The prepared medicine has the preparation forms such as the liquid preparation, the solid preparation, the capsule preparation or the gelatin pearl preparation, and the medication mode is oral medication or injection medication. The cortex meliae effective ingredients provided by the invention can be applied to the medicine for treating and preventing tumors. The preparation method of the invention has the advantages of simple and convenient operation and stable quality. In addition, no report about the liver-tumor-resistance activity of the cortex meliae appears in the prior art, and the invention provides the study basis for developing new cortex meliae anti-tumor medicine.

Description

technical field [0001] The invention belongs to a traditional Chinese medicine extract, and in particular relates to a preparation method for extracting effective components from neem bark, and the application of the effective components in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Pacl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/58A61P35/00A61P1/16A61K129/00
Inventor 程翼宇瞿海斌吴斌
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap