Method for preparing active ingredient of yerbadetajo herb and application

An effective component, the technology of Eclipta chinensis, which is applied in the direction of medical preparations, drug combinations, and pharmaceutical formulas containing active ingredients, can solve problems involving normal cells, side effects, etc., and achieve the effect of simple method and stable quality

Inactive Publication Date: 2011-12-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Method for preparing active ingredient of yerbadetajo herb and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The preparation of embodiment one Ecliptas active ingredient

[0012] Add 1500ml of ethyl acetate and ethanol (1:0.8) (volume ratio) after pulverizing 200g of Eclipta medicinal material, heat and extract once to obtain an extract, concentrate the extract into an extract, and use a reversed-phase silica gel column (ODS) to It is separated, first use 20% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 90% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluate Liquid II was concentrated and dried to obtain a sample; the obtained sample was further separated by preparative liquid chromatography, and the separation conditions of preparative chromatography were as follows: the chromatographic column was a preparative column, the mobile phase was water and acetonitrile, and gradient elution was performed. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% acet...

Embodiment 2

[0013] The preparation of embodiment two Ecliptas active components

[0014] Add ethyl acetate and ethanol (1:1.2) (volume ratio) 5000ml after pulverizing 500g of Eclipta medicinal material, heat and extract 3 times to obtain an extract, concentrate the extract into extract, and use a reversed-phase silica gel column (ODS) to It is separated, first use 40% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 100% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluate Liquid II was concentrated and dried to obtain a sample; the obtained sample was further separated by preparative liquid chromatography, and the separation conditions of preparative chromatography were as follows: the chromatographic column was a preparative column, the mobile phase was water and acetonitrile, and gradient elution was performed. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% aceton...

Embodiment 3

[0015] The preparation of embodiment three Ecliptas active components

[0016] Add 2000ml of ethyl acetate and ethanol (1:1) (volume ratio) after pulverizing 250g of Eclipta medicinal material, heat and extract twice to obtain an extract, concentrate the extract into extract, and use a reversed-phase silica gel column (ODS) to It is separated, first use 30% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 95% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluate Liquid II was concentrated and dried to obtain a sample; the obtained sample was further separated by preparative liquid chromatography, and the separation conditions of preparative chromatography were as follows: the chromatographic column was a preparative column, the mobile phase was water and acetonitrile, and gradient elution was performed. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% aceton...

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Abstract

The invention provides a method for preparing an active ingredient of yerbadetajo herb, which comprises the following steps of: heating and extracting medicinal materials, concentrating the extract, separating the extract with a silica gel column, eluting the extract, concentrating and drying the elute, continuously separating the elute by using a prepared liquid phase chromatograph, collecting the solution, and concentrating and drying the solution to obtain the active ingredient. The active ingredient of the yerbadetajo herb can be used as an active ingredient, and pharmaceutically acceptable medicinal excipient or carrier is added into the active ingredient to prepare a preparation according to a method recorded on pharmaceutics. The preparation form of the prepared medicament is liquid, solid, capsules or pills, and the administration mode is oral administration or injection administration. The active ingredient of the yerbadetajo herb provided by the invention can be applied in preparing medicaments for treating and preventing tumor. The preparation method has simple and convenient operation and stable quality, and provides a research foundation for researching and developingnew anti-tumor medicaments of the yerbadetajo herb because liver tumor activity resistance of the yerbadetajo herb is not yet reported in the prior art.

Description

technical field [0001] The invention belongs to traditional Chinese medicine extracts, and in particular relates to a preparation method for extracting effective components from Eclipta chinensis, and the application of the effective components in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61P35/00
Inventor 程翼宇瞿海斌吴斌
Owner ZHEJIANG UNIV
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