Preparation method and application of effective component of trumpetcreeper

An effective component, the technology of Ling Xiaohua, which is applied in the application field of preparation of drugs for treatment and prevention of tumors, can solve the problems of involving normal cells and side effects, and achieves the effect of simple method and stable quality.

Inactive Publication Date: 2012-06-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Preparation method and application of effective component of trumpetcreeper

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The preparation of embodiment one Ling Xiaohua effective components

[0012] Add 1500ml of ethyl acetate and ethanol (1:0.8) (volume ratio) after crushing 200g of Lingxiaohua medicinal material, heat and extract once to obtain an extract, concentrate the extract into extract, and separate it with a reversed-phase silica gel column. Use 20% (volume ratio) ethanol solution as mobile phase to obtain eluent I, discard it, then replace 90% (volume ratio) ethanol solution as mobile phase to obtain eluent II, and concentrate and dry eluent II Obtain the sample; Continue to separate the sample obtained by preparative liquid chromatography, the separation conditions of the preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, and gradient elution. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% acetonitrile solution; at 4 minutes, the mobile phase wa...

Embodiment 2

[0013] The preparation of embodiment two Ling Xiaohua effective components

[0014] After pulverizing 500g of Lingxiaohua medicinal material, add ethyl acetate and ethanol (1:1.2) (volume ratio) 5000ml, heat and extract 3 times to obtain an extract, concentrate the extract into an extract, and use a reversed-phase silica gel column (ODS) for its extraction. Separation, first use 40% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 100% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluent II The sample was obtained after concentration and drying; the obtained sample was further separated by preparative liquid chromatography, and the separation conditions of the preparative chromatography were as follows: the chromatographic column was a preparative column, the mobile phase was water and acetonitrile, and gradient elution was performed. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80...

Embodiment 3

[0015] The preparation of embodiment three Lingxiaohua effective components

[0016] After pulverizing 250g of Lingxiaohua medicinal material, add ethyl acetate and ethanol (1:1) (volume ratio) 2000ml, heat and extract twice to obtain an extract, concentrate the extract into an extract, and use a reversed-phase silica gel column (ODS) to carry out Separation, first use 30% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 95% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluent II The sample was obtained after concentration and drying; the obtained sample was further separated by preparative liquid chromatography, and the separation conditions of the preparative chromatography were as follows: the chromatographic column was a preparative column, the mobile phase was water and acetonitrile, and gradient elution was performed. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous s...

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Abstract

The invention provides a preparation method of an effective component of trumpetcreeper, which comprises the following steps: extracting a medicinal material by heating, concentrating, separating by a silica gel column, and eluting; concentrating and drying eluent, and continuing to separate by preparative liquid chromatography; and collecting solution, and obtaining the effective component afterthe solution is concentrated and dried. The effective component of the trumpetcreeper can be taken as an active ingredient and is added with medicinally accepted excipients or carriers to be preparedinto a preparation according to the method recorded in pharmacy, and the preparation of the prepared drug comprises a liquid preparation, a solid preparation, a capsule preparation or a pill preparation; and the administration comprises oral administration or injection administration. The effective component of the trumpetcreeper provided by the invention can be applied in the preparation of drugs for treating and preventing tumor. The preparation method of the invention has simple operation and stable quality, and no report that the trumpetcreeper can resist the activity of liver tumor exists in the prior art, so the preparation method provides a research basis for research and development of novel trumpetcreeper anti-tumor drugs.

Description

technical field [0001] The invention belongs to traditional Chinese medicine extracts, and in particular relates to a preparation method for extracting effective components from Lingxiaohua, and the application of the effective components in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Pac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P35/00
Inventor 程翼宇瞿海斌吴斌
Owner ZHEJIANG UNIV
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