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Microbial additive for alfalfa haylage, preparation method and application thereof

A microbial additive and semi-dry silage technology, which is applied to the preservation method, application, and animal feed of animal feed raw materials. It can solve the problems such as the inability to exclude air, and achieve the effects of accelerating the initial fermentation speed, improving the quality of silage, and promoting growth.

Inactive Publication Date: 2013-01-16
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a kind of microbial additive for alfalfa semi-dry silage aiming at the defects and deficiencies in the prior art that air cannot be eliminated. The microbial additive can increase the content of organic acids and decrease the pH value, inhibit the growth of harmful molds, and promote the growth of lactic acid bacteria. Achieved the purpose of rapidly reducing pH and improving silage quality

Method used

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  • Microbial additive for alfalfa haylage, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Cultivation of Aspergillus oryzae seeds: Activate Aspergillus oryzae in corn comprehensive culture medium, the activation temperature is 28°C, and the cultivation time is 4-5 days.

[0028] 2. Solid culture of Aspergillus oryzae: mix bran and corn flour in a weight ratio of 85%: 15%, adjust to a moisture content of 50% after mixing to obtain a solid medium, and mix the above-mentioned Aspergillus oryzae seed liquid Add it to the solid medium at a concentration of 0.5%, and cultivate it at 28°C for 24-48 hours to produce spores of Aspergillus oryzae, and the spore germination rate of the spores is >99%. After cultivation, each gram of solid medium contains at least 10 spores 9 indivual.

[0029] 3. Preparation of Aspergillus oryzae bacterial suspension: Dissolve the above 10 grams of solid medium in 100 milliliters of sterile water, so that each milliliter of bacterial suspension contains at least 10 8 spores.

[0030] 4. Activation of Streptococcus lactis: Put Str...

Embodiment 2

[0036] 1. Cultivation of Aspergillus oryzae seeds: Activate Aspergillus oryzae in corn comprehensive culture medium, the activation temperature is 28°C, and the cultivation time is 4-5 days.

[0037] 2. Solid culture of Aspergillus oryzae: mix bran and corn flour in a weight ratio of 85%: 15%, adjust to a moisture content of 50% after mixing to obtain a solid medium, and mix the above-mentioned Aspergillus oryzae seed liquid Add it to the solid medium at a concentration of 0.5%, and cultivate for 24 to 48 hours to produce green spores with a spore germination rate of >99%. After cultivation, each gram of solid medium contains at least 10 spores 9 indivual.

[0038] 3. Preparation of Aspergillus oryzae bacterial suspension: Dissolve 10 grams of solid medium in 100 milliliters of sterile water so that each milliliter of bacterial suspension contains at least 10 8 spores.

[0039] 4. Activation of Streptococcus lactis: Put Streptococcus lactis in MRS liquid medium for activa...

Embodiment 3

[0045] 1. Cultivation of Aspergillus oryzae seeds: activate Aspergillus oryzae in corn comprehensive culture medium, the activation temperature is 28°C, and the cultivation time is 4-5 days.

[0046] 2. Solid culture of Aspergillus oryzae: mix bran and corn flour in a weight ratio of 85%: 15%, adjust to a moisture content of 50% after mixing to obtain a solid medium, and mix the above-mentioned Aspergillus oryzae seed liquid Add it to the solid medium at a concentration of 0.5%, and cultivate for 24 to 48 hours to produce green spores with a spore germination rate of >99%. After cultivation, each gram of solid medium contains at least 10 spores 9 indivual.

[0047] 3. Preparation of Aspergillus oryzae bacterial suspension: Dissolve 10 grams of solid medium in 100 milliliters of sterile water so that each milliliter of bacterial suspension contains at least 10 8 spores.

[0048] 4. Activation of Streptococcus lactis: Put Streptococcus lactis in MRS liquid medium for activat...

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Abstract

The invention discloses a microbial additive for alfalfa haylage, a preparation method and an application thereof. The additive is prepared from a bacterial suspension of aspergillusoryzae and an MRS liquid nutrient medium for streptococcus lactis according to the ratio of 50-70%: 50-30% in weight percent, wherein per milliliter of the bacterial suspension of the aspergillusoryzae at least contains 1.0*108 aspergillusoryzae spores, and per milliliter of the MRS liquid nutrient medium at least contains 4.0*108 cfu streptococcus lactis. The microbial additive quickens the initial fermentation rate of lactobacillus, enhances acid production capability, effectively inhibits the activity of saccharomycetes and other undesirable microorganism, strengths haylage effect and effectively promotes the production performance of cows, beef cattle, mutton sheep and swine. The strain adopted by the invention can be obtained easily, can be produced massively on application and has practicability.

Description

technical field [0001] The invention relates to a microbial additive, in particular to a microbial additive for alfalfa semi-dry forage silage. Background technique [0002] Alfalfa is a perennial leguminous forage with rich nutritional value. It is a high-quality forage for herbivores and has the reputation of "the king of forage". For a long time, the main product of alfalfa production is hay, but in many places in my country, there are losses such as rain and fallen leaves during the preparation process of alfalfa hay, and the general loss rate is about 30%. Especially in the main producing areas of alfalfa in my country, due to the same period of rain and heat, the loss rate of alfalfa due to rain during the harvest season is higher. Moreover, it is difficult to make high-quality hay by cutting 2 or 3 stubbles of alfalfa in the main alfalfa producing areas in northern my country. The production of dehydrated alfalfa by drying is almost not affected by the rainy season a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K3/03
Inventor 赵红波王星凌盛清凯胡明游伟
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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