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Epoxy mesoporous molecular sieve for use in bio-enzyme immobilization and preparation method thereof

An epoxy-based mesoporous and mesoporous molecular sieve technology, which is applied in the direction of being fixed on or in an inorganic carrier, can solve the problems of loss of catalytic activity of free enzymes, and achieve the effects of improving operational stability, performance and activity.

Inactive Publication Date: 2010-10-20
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses a large amount of organic solvents in the process of preparing immobilized enzymes, which easily causes the free enzymes to lose their catalytic activity.

Method used

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  • Epoxy mesoporous molecular sieve for use in bio-enzyme immobilization and preparation method thereof
  • Epoxy mesoporous molecular sieve for use in bio-enzyme immobilization and preparation method thereof
  • Epoxy mesoporous molecular sieve for use in bio-enzyme immobilization and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Schematic diagram of the functionalization of epoxy groups on the surface of mesoporous molecular sieves and the immobilization of enzymes with vinyltrimethoxysilane and m-CPBA. figure 1 As shown, at room temperature, 5.68g Pluronic P123 (EO 20 PO 70 EO 20 ,M av =5800) and 11.36gNa 2 SO 4 Dissolve in 160mL of 1.0mol / L HCl solution; after complete dissolution, raise the temperature of the solution to 35°C and keep stirring for several hours, slowly add 53.2mmol of tetraethyl orthosilicate (TEOS) and vinyltriethoxy A mixture of silane (TEVS) (the molar percentage of TEVS is 5%), and kept stirring at 35°C for 20 hours; the solution was transferred to a hydrothermal crystallization kettle with a polytetrafluoroethylene liner, and placed in Crystallize in an oven at 100°C for 24 hours, then cool the solution to room temperature and filter the obtained solid, and dry it in an oven at 100°C overnight; put 1.0 g of mesoporous molecular sieves without template removal in a ...

Embodiment 2

[0038] The molar percentage of TEVS in embodiment 1 is changed from 5% to 10%, other conditions are the same as embodiment 1, obtain epoxy-based mesoporous molecular sieve, be used for the immobilization of penicillin acylase, the immobilized enzyme activity that obtains is 2262U / g, after 10 cycles of use, the immobilized enzyme retained 80% of its initial activity.

Embodiment 3

[0040] Change the molar percentage of TEVS in Example 1 from 5% to 15%, and other conditions are the same as in Example 1 to obtain epoxy-based mesoporous molecular sieves, which are used for the immobilization of penicillin acylase, and the immobilized enzyme activity obtained is 1563U / g, after 10 cycles of use, the immobilized enzyme retained 66% of its initial activity.

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Abstract

The invention discloses an epoxy mesoporous molecular sieve for use in bio-enzyme immobilization and a preparation method thereof. In the invention, cyclo groups of the mesoporous molecular sieve which contains vinyl and has a cubic phase Ia3d structure and the mesoporous molecular sieve which has a surface vinyl function are oxidized by using metachloroperbenzoic acid to introduce epoxy functional groups with a shorter chain length onto the surface of the mesoporous molecular sieve while the influences of a surface functionization process on the aperture, the specific surface area and the pore volume of the mesoporous molecular sieve are reduced as much as possible, so the bio-enzyme can be directly immobilized on the surface of the mesoporous molecular sieve in a covalent bonding form without further activation, and the performance of the immobilized enzyme can be improved. The epoxy mesoporous molecular sieve can be used for the immobilization of water soluble bio-enzymes such as penicillin acylase, glucose isomerase, glucosidetransferase, parenzyme and diastase, in particular for the immobilization of the penicillin acylase; and the activity of the obtained immobilized enzyme is 8416U / g, and 90 percent of original activity of the immobilized enzyme is retained after 10 times of recycling.

Description

technical field [0001] The present invention relates to an epoxy-based mesoporous molecular sieve for immobilization of biological enzymes and a preparation method thereof, specifically, by using m-chloroperoxybenzoic acid on the mesoporous molecular sieve and the surface of the cubic phase Ia3d structure containing vinyl The epoxidation of vinyl-functionalized mesoporous molecular sieves introduces epoxy functional groups with shorter chain lengths on the surface of mesoporous molecular sieves, and then immobilizes biological enzymes on the surface of mesoporous molecular sieves in a covalent manner to improve the activity of immobilized enzymes. performance. Background technique [0002] There are many deficiencies in using free enzyme directly in the catalytic process, such as instability in high temperature, strong acid, strong alkali and organic solvent, and easy loss of catalytic activity; free enzyme recovery is difficult, economically unreasonable, and the product is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14
Inventor 陈栋郭杨龙杨波王通海高振源卢冠忠詹望成郭耘王筠松
Owner EAST CHINA UNIV OF SCI & TECH
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