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Sensor for detecting penicillin in liquid

A technology of penicillin and sensors, applied in the field of biosensors, can solve problems such as prone to false positive results, high requirements for instruments, cumbersome operations, etc., and achieve the effects of improving detection sensitivity, high sensitivity, and simplifying operating procedures

Inactive Publication Date: 2010-10-13
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can detect antibiotic residues in milk, they also have shortcomings such as time-consuming, manpower-consuming, heavy workload, high requirements for instruments, cumbersome operations, and prone to false positive results.

Method used

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  • Sensor for detecting penicillin in liquid
  • Sensor for detecting penicillin in liquid
  • Sensor for detecting penicillin in liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Prepare the sensor for detecting penicillin in liquid according to the following steps:

[0042] a. Use 1.0μm, 0.3μm, 0.05μm Al on the front end surface of the platinum disk electrode in sequence 2 o 3 The powder was polished into a mirror surface, washed with double distilled water at each step, and then the platinum disk electrodes were respectively placed in HNO 3 , anhydrous ethanol and double-distilled water for 15 minutes, and the treated electrode was dried at room temperature for later use; egg yolk lecithin and cholesterol were prepared into a BLM film-forming solution at a mass ratio of 5:2, and 5 μl of BLM film-forming solution Use a micro-sampler to drop on the surface of the cleaned electrode, keep it at room temperature for 20 minutes, and then insert the electrode into 0.1mol / L KCl solution and soak for 15 minutes to prepare the BLM-modified platinum disk electrode and the BLM-modified platinum disk The electrode is represented by Pt-BLM;

[0043] b. I...

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Abstract

The invention provides a sensor for detecting penicillin in liquid. A method for preparing the sensor comprises the following steps of: electroplating nanogold on a platinum disk electrode modified by a bilayer lipid membrane; and combining penicillin antibody on the platinum disk electrode modified by the nanogold bilayer lipid membrane to make the platinum disk electrode modified by the nanogold bilayer lipid membrane and combined with the penicillin antibody. The sensor has high sensitivity and an acceptable linear range and can save the time and simplify the operating procedure.

Description

technical field [0001] The invention belongs to the field of biosensors, and relates to a biosensor for detecting antibiotic residues, in particular to a nano-bilayer lipid membrane (Nano-BLM) biosensor for detecting antibiotic residues in food. Background technique [0002] Penicillin belongs to the class of β-lactam antibiotics that are frequently used in veterinary therapy to prevent bacterial infections. This leaves penicillin residues in the milk and certain amounts of penicillin can cause allergic reactions in some people. At the same time, penicillin residues will also promote the emergence of drug-resistant strains of bacteria. In order to avoid risks from residues in milk or from edible tissues obtained from treated animals, countries have established maximum residue limits (MRLs). For example, EU Regulation 508 / 1999 sets the MRL for penicillin in milk at 4 ppb or 1.2×10 -8 M. At present, the detection methods of antibiotic residues in milk are generally as foll...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 李媛媛许冰夏玮周寅徐顺清
Owner HUAZHONG UNIV OF SCI & TECH
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