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Preparation method for Flos farfarae active constituent and application thereof

A technology for effective components and winter flowers, which is applied in the application field of preparation of drugs for treatment and prevention of tumors, can solve the problems of involving normal cells and side reactions, and achieves the effect of simple method and stable quality.

Inactive Publication Date: 2010-10-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Preparation method for Flos farfarae active constituent and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The preparation of embodiment one winter flower effective component

[0012] After pulverizing 200g of winter flower medicinal material, add 1500ml of ethyl acetate and ethanol (1:0.8) (volume ratio), heat and extract once to obtain an extract, concentrate the extract into an extract, and use a reversed-phase silica gel column (ODS) to extract it. Carry out separation, first use 20% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 90% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluate II Concentrated and dried to obtain a sample; continue to separate the sample obtained by preparative liquid chromatography, the separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, and gradient elution. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% acetonitrile solution...

Embodiment 2

[0013] The preparation of embodiment two winter flower effective components

[0014] After crushing 500g of winter flower herbs, add ethyl acetate and ethanol (1:1.2) (volume ratio) 5000ml, heat and extract 3 times to obtain extract, concentrate the extract into extract, and use reversed-phase silica gel column (ODS) to extract Carry out separation, first use 40% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 100% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluate II Concentrated and dried to obtain a sample; continue to separate the sample obtained by preparative liquid chromatography, the separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, and gradient elution. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% acetonitrile solution; at 4 minutes, the mobile...

Embodiment 3

[0015] The preparation of embodiment San Donghua effective component

[0016] After pulverizing 250g of winter flower herbs, add 2000ml of ethyl acetate and ethanol (1:1) (volume ratio), heat and extract twice to obtain an extract, concentrate the extract into an extract, and use a reversed-phase silica gel column (ODS) to extract it. Carry out separation, first use 30% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 95% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluate II Concentrated and dried to obtain a sample; continue to separate the sample obtained by preparative liquid chromatography, the separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, and gradient elution. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aqueous solution and 20% acetonitrile solution; at 4 minutes, the ...

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Abstract

The invention provides a p reparation method for a Flos farfarae active constituent, which comprises the following steps that medicinal materials are extracted through heating, are concentrated, are separated through a silica gel column method and are eluted, eluent is concentrated and is dried, separation is continuously conducted by using a liquid chromatography, and solution is collected, is concentrated and is dried to obtain the active constituent. The Flos farfarae active constituent of the invention can be used as an active ingredient and can be prepared into preparations according to methods recorded in pharmaceutics through adding pharmaceutically acceptable pharmaceutical excipient or carriers. The preparations of the prepared medicines comprise liquid preparations, solid preparations, capsules or pills, and the dosage is oral administration or injection. The Flos farfarae active constituent of the invention can be applied to the preparation of medicines for treating and preventing tumors. The preparation method has the advantages of simple and convenient operation and stable quality. No report on Flos farfarae with activity of fighting against liver tumor exists in the prior art and a research foundation is provided for the research and the development of novel Flos farfarae anti-tumor medicines.

Description

technical field [0001] The invention belongs to traditional Chinese medicine extracts, and in particular relates to a preparation method for extracting effective components from winter flowers, and the application of the effective components in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P35/00
Inventor 程翼宇瞿海斌吴斌
Owner ZHEJIANG UNIV
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