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Test paper for detecting newcastle disease and infectious bronchitis viruses in one step

A technology for bronchitis and Newcastle disease, applied to measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple operation, high sensitivity and low cost

Inactive Publication Date: 2010-10-06
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the immunodiafiltration method, it has good stability, easier and faster operation, and because it is in the form of dry test strips, it does not need low-temperature storage and is convenient for storage and transportation. detection tools have not been reported

Method used

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  • Test paper for detecting newcastle disease and infectious bronchitis viruses in one step
  • Test paper for detecting newcastle disease and infectious bronchitis viruses in one step

Examples

Experimental program
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Embodiment Construction

[0010] To prepare the test strip of the present invention, the bottom liner 1, the sample pad 2 and the absorbent paper 5 are common components in the field, and the preparation methods of the remaining components are as follows:

[0011] 1. Preparation of coating film 4:

[0012] 1. Antibody cell line preparation

[0013] ① Preparation and verification of monoclonal antibody cell lines against Newcastle disease virus

[0014] Slowly stir the chicken embryo allantoic fluid containing Newcastle disease virus, and add sodium chloride to make the final concentration 0.5mol / L, and then add 10% (weight percentage, the same below) polyethylene glycol 6000 (PEG6000) , 4℃ overnight, centrifuge at 8000r / min for 30 minutes, collect the virus precipitate, dissolve the virus precipitate in phosphate buffered saline (PBS), 4℃ overnight, centrifuge at 10000r / min for 1 hour, the resulting precipitate is the concentrated virus, Store in a -20℃ low-temperature refrigerator for later use.

[0015] Take...

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Abstract

The invention discloses a test paper for detecting newcastle disease and infectious bronchitis viruses in one step and a preparation method and application thereof. The preparation method comprises the following steps of: preparing a coating membrane, namely diluting monoclonal antibody or polyclonal antibody against the newcastle disease and infectious bronchitis viruses and mouse IgG against antibody by using coating buffer solution, spraying the three antibodies in parallel on the middle part of a nitrocellulose membrane respectively, and forming a detection line for chicken infectious bronchitis virus, a detection line for newcastle disease virus and a control line against mouse IgG after the three antibodies permeate into the nitrocellulose membrane; preparing a glass fiber membrane coated with gold-labeled antibody; sticking the coating membrane on the middle part on a bottom lining, and sticking the glass fiber membrane and a piece of water absorption paper on the left and right on the coating membrane; and sticking a sample pad on the glass fiber membrane. The test paper has the advantages of strong specificity, high sensitivity, high detection speed, low cost, simple and convenient operation and wide application.

Description

Technical field [0001] The invention belongs to the field of preventive veterinary inspection, and specifically relates to a test strip for one-step detection of chicken Newcastle disease and infectious bronchitis virus, and a preparation method and application thereof. Background technique [0002] Newcastle Disease (ND) has always been a disease that has caused major harm to the chicken industry. It has been listed as a Class A infectious disease by the International Veterinary Office (OIE). The disease has a greater commercial impact on world trade. Infectious Bronchitis (IB) is an acute infectious disease caused by infectious bronchitis virus (IBV) that harms the respiratory and genitourinary tracts. Once a flock is infected, it causes huge economic losses. At present, some new changes have taken place in the outbreak of chicken diseases. In the past, the occurrence of a single disease is rare. Instead, mixed infections of several diseases, viral diseases, and recessive chron...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/577G01N33/531
Inventor 高亚东凌红丽蒋贻海宋新雨魏笑笑
Owner QINGDAO VLAND BIOTECH INC
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