Method for preparing donkey skin collagens

A donkey skin and collagen technology, applied in the field of donkey skin collagen preparation, can solve the problems of poor water solubility, complex components of donkey-hide gelatin, low antigenicity, etc., and achieve low biodegradability, good skin homology and compatibility, Good water solubility

Active Publication Date: 2010-10-06
河北柯瑞生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The main purpose of the present invention is to solve the problems in the above-mentioned prior art that the application of donkey skin is limited to boiled donkey-hide gelatin, while the composition of donkey-hide gelatin is complicated, the water solubility is poor, and other applications are relatively limited except as oral medicine, and so on, to provide a The preparation method of the donkey skin collagen, using donkey skin as raw material, through specific extraction and refining methods, to obtain donkey skin collagen with good water solubility, not only retains biological activity, homology and compatibility with human body and skin, and It has the characteristics of biodegradability and low antigenicity, and can be diluted with water in any proportion. It can be used to prepare surgical implants and tissue engineering medical products, and develop medicines, medical reagents, and health products, etc.

Method used

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  • Method for preparing donkey skin collagens
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  • Method for preparing donkey skin collagens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] In this example, donkey skin collagen was prepared by a method including the following steps:

[0045] (1) Hair removal and degreasing:

[0046] Use fresh donkey skins that have passed inspection and quarantine as raw materials. After dehairing and degreasing, wash them with water until they are neutral, and store them in the refrigerator below 0°C for later use;

[0047] (2) Crushed skin, soaked in lye:

[0048] Thaw the spare donkey skin after treatment in the above step (1), break the skin into 20-40 mesh particles, take 500g donkey skin particles, add 6000ml sodium hydroxide solution with pH 7.5, soak and soften for 96h; rinse with deionized water to The pH is about 6.0, and neutral donkey skin particles are obtained;

[0049] (3) Low temperature enzymatic hydrolysis:

[0050] Take 200g of the neutral donkey skin granules treated in the above step (2), add 9000ml of hydrochloric acid solution with pH 6.5, and then add 8UI pepsin, put it at a low temperature below 10°C for 3 d...

Embodiment 2

[0058] In this example, donkey skin collagen was prepared by a method including the following steps:

[0059] (1) Hair removal and degreasing:

[0060] Use fresh donkey skins that have passed inspection and quarantine as raw materials. After dehairing and degreasing, wash them with water until they are neutral, and store them in the refrigerator below 0°C for later use;

[0061] (2) Crushed skin, soaked in lye:

[0062] Thaw the spare donkey skin after treatment in the above step (1), break the skin into 20-40 mesh particles, take 500g donkey skin particles, add 4000ml sodium hydroxide solution with pH 9.5, soak and soften for 48h; rinse with deionized water until The pH is about 7.0, and neutral donkey skin particles are obtained;

[0063] (3) Low temperature enzymatic hydrolysis:

[0064] Take 200g of the neutral donkey skin granules treated in the above step (2), add 10000ml of hydrochloric acid solution with pH 4.3, and then add 10UI pepsin, place at a low temperature below 10°C for...

Embodiment 3

[0070] In this example, donkey skin collagen was prepared by a method including the following steps:

[0071] (1) Hair removal and degreasing:

[0072] Use fresh donkey skins that have passed inspection and quarantine as raw materials. After dehairing and degreasing, wash them with water until they are neutral, and store them in the refrigerator below 0°C for later use;

[0073] (2) Crushed skin, soaked in lye:

[0074] Thaw the spare donkey skin after treatment in the above step (1), break the skin into 20-40 mesh particles, take 500g donkey skin particles, add 5000ml sodium hydroxide solution with pH 8.0, soak and soften for 72h; rinse with deionized water to The pH is about 6.5, and neutral donkey skin particles are obtained;

[0075] (3) Low temperature enzymatic hydrolysis:

[0076] Take 200g of the neutral donkey skin granules treated in the above step (2), add 9500ml of pH 4.5 hydrochloric acid solution, and then add 8UI pepsin, leave it at a low temperature below 10°C for 3 days...

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Abstract

The invention discloses a method for preparing donkey skin collagens. In the method, fresh donkey skin is used as a raw material, and modified donkey skin collagen products are modified by steps of unhairing, degreasing, crushing, alkali solution soaking, low-temperature enzymolysis, purification, modification and the like. The donkey skin collagen products prepared by the method is characterized by retaining a triple helical structure, possessing biological activity, being homologous and compatible with human organisms and skin, along with high biological degradability, low antigenicity and the like; and the donkey skin collagens can be diluted by water in any ratio and be used for preparing surgical implants and tissue engineering medical products and developing medicaments, medical preparations, healthcare products and the like.

Description

technical field [0001] The invention belongs to the technical field of collagen preparation, in particular to a method for preparing donkey skin collagen. Background technique [0002] Collagen is the most abundant and widely distributed protein family in animals, widely present in all tissues from lower vertebrates to mammals; it has biocompatibility, biodegradability and biological properties unmatched by other synthetic materials. active. At least 30 genes encoding collagen chains have been discovered, which can form more than 16 collagen molecules. According to their distribution and functional characteristics in the body, collagen is currently divided into interstitial collagen, basement membrane collagen and peripheral collagen. Interstitial collagen molecules account for the vast majority of collagen in the entire body, including type I, type II, and type III collagen Molecules, which are mainly distributed in skin, tendon and other tissues; basement membrane collag...

Claims

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Application Information

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IPC IPC(8): C08H1/06A61P7/04A61P7/06
Inventor 杨信诚李国英雷小林廖知恒陈丽
Owner 河北柯瑞生物医药有限公司
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