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Method for producing whole neisseria meningitidis antigen coated enzyme-labeled reaction plate and enzyme-linked immuno sorbent assay (ELISA) test kit

A technology for Neisseria meningitidis and detection kits, applied in measuring devices, color/spectral characteristic measurements, instruments, etc., can solve the problem of no uniform quality standards, uneven quality of immunodiagnostic kits, and disorderly production and application And other issues

Active Publication Date: 2010-09-29
赵俊 +1
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  • Application Information

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Problems solved by technology

[0004] At present, the quality of various immunodiagnostic kits for Neisseria meningitidis in the domestic market is uneven, there is no uniform quality standard, and the production and application are out of order, which has caused serious obstacles to the prevention, diagnosis and treatment of Neisseria meningitidis
At present, although foreign reagent manufacturers provide similar commercial diagnostic kits for detection, the price of such kits is very expensive, which limits the widespread clinical use

Method used

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  • Method for producing whole neisseria meningitidis antigen coated enzyme-labeled reaction plate and enzyme-linked immuno sorbent assay (ELISA) test kit

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Embodiment Construction

[0024] (1), Reagent

[0025] Coating solution (0.05mol / L, sodium carbonate-sodium bicarbonate buffer solution with pH9.6): Na 2 CO 3 1.5g, NaHCO 3 2.9g, Na 2 N 3 0.2g, add double distilled water to 1000ml, adjust to pH9.6;

[0026] Specimen diluent (0.01mol / L phosphate buffered saline (PBS) pH7.4 containing 10% calf serum and 2% anti-human IgG), PBS: NaCl 8.0g, KH 2 PO 4 0.2g, Na 2 HPO 4 12H 2 O 2.9g, KCl 0.2g, thimerosal 0.1g, add double distilled water to 1000ml, adjust to pH 7.4, and get it;

[0027] Blocking solution: (10% calf serum / PRS solution) calf serum 100ml, 1×PBS (pH7.4) 900ml;

[0028] Wash solution (PBST, pH7.4): NaCl 8.0g, KH 2 PO 4 0.2g, Na 2 HPO 4 12H 2 O 2.9g, KCl0.2g, Tween 200.5ml, thimerosal 0.1g, add double distilled water to 1000ml, adjust to pH7.4;

[0029] Enzyme-labeled secondary antibody (HRP*mouse anti-human IgM monoclonal antibody): horseradish peroxidase-labeled mouse anti-human IgM monoclonal antibody;

[0030] Substrate sol...

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Abstract

The invention discloses a method for producing a whole neisseria meningitidis antigen coated enzyme-labeled reaction plate and an enzyme-linked immuno sorbent assay (ELISA) test kit. The whole neisseria meningitidis antigen coated enzyme-labeled reaction plate is produced by mixing an equal amount of A-type neisseria meningitides and C-type neisseria meningitides and coating the mixture on an enzyme-labeled reaction plate and is used for conveniently and rapidly detecting the specific neisseria meningitides IgM antibody in the serum with the indirect ELISA. The ELISA test kit comprises the whole neisseria meningitidis antigen coated enzyme-labeled reaction plate, sample diluent, washing liquid, color rendering solution and stop solution. The invention discloses a method for sensitively and rapidly capturing the specific neisseria meningitides IgM antibody in the serum with the ELISA. The ELISA test kit has high specificity and stability and is mainly used for the laboratory research and the clinical diagnosis.

Description

technical field [0001] The invention relates to the detection of Neisseria meningitidis, in particular to a method for preparing a Neisseria meningitidis whole bacterial antigen-coated enzyme-labeled reaction plate and an ELISA detection kit. Background technique [0002] Neisseria meningitidis is a Gram-negative diplococcus, and epidemic cerebrospinal meningitis (referred to as meningitis) caused by respiratory infection is a serious infectious disease that is harmful to humans. In addition, it can also cause bacteremia , pneumonia, arthritis, conjunctivitis, myocarditis, urinary system and other inflammations have caused many pandemics in the world, and they occur almost every year in our country, mainly in spring or winter. and multiple diseases. Neisseria meningitidis can be divided into 13 serogroups including A, B, C, D, H, I, K, L, X, Y, Z, 29E and W 135, most of which are pathogenic to humans. , Group C, and other groups are often found in carriers, but rarely caus...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/569G01N21/31
Inventor 赵俊王明丽李京培
Owner 赵俊
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