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Organic-inorganic hybrid monolithic material and application thereof in immobilized enzyme reactor

A technology of monolithic materials and immobilized enzymes, applied in the direction of immobilized enzymes, enzymes, biochemical equipment and methods, etc., to achieve the effects of good regeneration, good permeability, and high enzyme activity

Inactive Publication Date: 2010-09-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is difficult to regenerate such enzyme reactors due to the stability of the covalent bond

Method used

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  • Organic-inorganic hybrid monolithic material and application thereof in immobilized enzyme reactor
  • Organic-inorganic hybrid monolithic material and application thereof in immobilized enzyme reactor
  • Organic-inorganic hybrid monolithic material and application thereof in immobilized enzyme reactor

Examples

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Effect test

Embodiment Construction

[0017] A renewable monolithic material-immobilized enzyme reactor was prepared according to the following steps.

[0018] 1. The preparation of the overall material carrier, the specific process is:

[0019] 1) React 2-glycidyloxypropyltrimethoxysilane (GLYMO) with a reagent containing metal ion chelating groups of N, S or O atoms in a water bath to prepare solution A. The specific parameters of the synthesis example are shown in Table 1 below.

[0020] 2) Tetraethoxysilane (TEOS) or tetramethoxysilane (TMOS), methanol or ethanol, hydrochloric acid and H 2 The O mixture was reacted in a water bath to form solution B. The specific parameters of the synthesis example are shown in Table 2 below.

[0021] 3) Mix solution A and solution B, add template molecule cetyltrimethylammonium bromide (CTAB) and catalyst octylamine, prepare a kind of surface with metal ion chelating group through sol-gel method Monolithic material carrier. The specific parameters of the synthesis exampl...

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Abstract

The invention relates to a monolithic material immobilized enzyme reactor, in particular to an organic-inorganic hybrid monolithic material and application thereof in the immobilized enzyme reactor. Methyl orthosilicate or tetraethyl orthosilicate, and 2-glycidoxypropyl trimethoxysilane are taken as precursor molecules; an iminodiacetic acid is taken as a metal ion chelating agent; cetyl trimethyl ammonium bromide serves as template molecules; octylamine is taken as a catalyst; monolithic material carriers are prepared by a sol-gel method, wherein the surface of the monolithic material carrier carries metal ion chelating groups; and then the reproducible monolithic material immobilized enzyme reactor can be prepared by sequentially and continuously making the monolithic material carriers pass through the solution of transition metal ions and enzyme solution. The prepared enzyme reactor has the advantages of relatively higher reproducibility, reusability, high activity, high permeability, low reaction pressure and high mechanical strength; and the preparation method is simple and convenient.

Description

technical field [0001] The invention relates to a monolithic material immobilized enzyme reactor, in particular to a reproducible organic-inorganic hybrid monolithic material immobilized enzyme reactor, which has better reproducibility and higher enzyme activity. Background technique [0002] Enzyme immobilization refers to the immobilization of the target enzyme molecule on a specific carrier through adsorption, embedding, cross-linking, covalent bonding and other methods to exert the biocatalytic function of the enzyme. Compared with enzyme-catalyzed reactions in free solution, immobilized enzymes have the advantages of higher enzyme / substrate ratio and faster enzymatic hydrolysis rate. Therefore, the immobilized enzyme reactor technology has received extensive attention from many researchers in recent years (Svec, F. Electrophoresis 2006, 27, 947-961 & Ma, J.F.; Zhang, L.H.; et al., Anal. Chem. Acta.2009 , 632, 1-8). [0003] The performance of immobilized enzymes is cl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/00
Inventor 张丽华马俊锋梁振张维冰张玉奎
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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