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Composite of all-trans-retinoic acid and liposome and application thereof

A technology of all-trans retinoic acid and complexes, which is applied in the direction of liposome delivery, active ingredients of hydroxyl compounds, medical preparations of non-active ingredients, etc., and can solve the problem of weak inhibitory effect of tumor stem cells

Inactive Publication Date: 2010-09-29
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, traditional radiotherapy and chemotherapy can only kill most of the normal tumor cells, but show a weak inhibitory effect on tumor stem cells

Method used

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  • Composite of all-trans-retinoic acid and liposome and application thereof
  • Composite of all-trans-retinoic acid and liposome and application thereof
  • Composite of all-trans-retinoic acid and liposome and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1, preparation and identification of the complex of all-trans retinoic acid and liposome

[0039] method one,

[0040] 1. Preparation

[0041] Complexes of all-trans retinoic acid and liposomes were prepared by thin-film dispersion and extrusion through membranes. Egg yolk lecithin (EPC) (purchased from NOF Co., Ltd., Lot No: 108057-3), cholesterol (purchased from Beijing Haidian District Microbial Culture Culture Products Factory) and PEG2000-DSPE (purchased from NOF Co., Ltd., Lot No. No: M86563) as a fat material, the molar ratio is 59:39:4.

[0042] (1) Dissolution: Lipid material and all-trans retinoic acid (purchased from Beijing Beileys Biochemical Co., Ltd., CAS No.: 302-79-4) (lipid material: all-trans retinoic acid = 20:1, mass / quality) put after dissolving with chloroform in eggplant-shaped bottle, rotary evaporation removes chloroform, and the product obtained is recorded as lysate. This step produces a lipid film, which forms a mixture with...

Embodiment 2

[0065] Example 2, Functional identification of complexes of all-trans retinoic acid and liposomes

[0066] 1. Breast cancer cell culture and complex uptake detection

[0067] MCF-7 breast cancer cells (purchased from the Institute of Materia Medica, Chinese Academy of Medical Sciences) were cultured with RPMI-1640 medium containing 10% fetal bovine serum (Maichen Technology Beijing Co., Ltd., catalog number: CM-10041); MDA-MB- 231 breast cancer cells (purchased from the Institute of Materia Medica, Chinese Academy of Medical Sciences) were cultured with Leibovitz's L-15 culture medium containing 10% fetal bovine serum (Maichen Technology Beijing Co., Ltd., catalog number: CM-10045); the culture of two kinds of cells All conditions are 5% CO 2 , cultivated at 37°C.

[0068] Uptake of complexes of all-trans retinoic acid and liposomes by breast cancer cells observed by laser scanning confocal microscopy. The complex of all-trans retinoic acid and liposome obtained in Example ...

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Abstract

The invention discloses a composite of all-trans-retinoic acid and liposome and application thereof. The composite of all-trans-retinoic acid and liposome consists of the liposome and the all-trans-retinoic acid encapsulated in the liposome. The composite is prepared by a method comprising: 1) dissolving lipid materials and the all-trans-retinoic acid to prepare a mixture of a lipid membrane and the all-trans-retinoic acid; 2) hydrating the mixture of the liquid membrane and the all-trans-retinoic acid to prepare a hydration product; and 3) performing ultrasonic on the hydration product to prepare the composite of the all-trans-retinoic acid and the liposome. Experiments of the invention prove that the composite of the all-trans-retinoic acid and the liposome can be used for inhibiting the reproduction of tumor cells, and the combined application of the composite of the all-trans-retinoic acid and the liposome and Vinorelbine invisibile liposome produces the strongest inhibitory effect on the recrudesce of tumors.

Description

technical field [0001] The invention relates to a compound of all-trans retinoic acid and liposome and its application in the field of biotechnology. Background technique [0002] Cancer stem cells play an important role in the occurrence, metastasis and recurrence of tumors, and tumor recurrence is the main cause of tumor treatment failure. Similar to stem cells in normal tissues, cancer stem cells have the ability to self-renew and differentiate (ie, become more mature tumor cells). In normal tissue, one stem cell divides into another stem cell and a differentiated cell, so that the number of stem cells remains constant and strictly limited. However, tumor stem cells can divide into multiple new tumor stem cells, and the continuation of this process will lead to an increase in the number of tumor cells and cause tumor recurrence. There is evidence of CD44 + / CD24 - Phenotyped breast cancer stem cells have strong tumorigenic ability, an example is that only a small amou...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/203A61K47/34A61K31/475A61P35/00A61P15/14A61K47/10
Inventor 吕万良李若婧张燕应雪姚红娟
Owner PEKING UNIV
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