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Bursin heptapeptide with immune regulation effect

An immunomodulation, bursa of fabric technology, applied in the field of immunology, can solve problems such as the loss of promoting PFC activity, and achieve the effect of promoting cellular immune response, no toxic side effects, and extremely weak immunogenicity

Inactive Publication Date: 2012-02-01
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

80°C or proteolytic enzyme treatment, the PFC-promoting activity of the extract was lost

Method used

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  • Bursin heptapeptide with immune regulation effect
  • Bursin heptapeptide with immune regulation effect
  • Bursin heptapeptide with immune regulation effect

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Embodiment 1

[0031] Implementation 1. Separation and identification of bursalin heptapeptide:

[0032] A method for extracting bursa heptapeptide, comprising the following steps: take 2000 grams of bursae of healthy 4-6 week-old chickens (AA broiler chicken, farm affiliated to Shanghai Academy of Agricultural Sciences), crush them with 3000ml of normal saline, mix Evenly, repeated freezing and thawing three times. Centrifuge at 14,000g for 60 minutes at 4°C, transfer the supernatant to another tube, and centrifuge again. Then through a 1000Da ultrafiltration membrane, ultrafiltration, and freeze-drying the filtrate. The lyophilized sample was diluted with solution B (2% acetonitrile, 0.065% trifluoroacetic acid), centrifuged at 12000g for 10 minutes, and then filtered with a 0.22um filter membrane. Get the filtrate and analyze through the reverse high performance liquid phase purification, the harvest retention time is the active peak of 23.98 minutes (see figure 1 ). The eluate of har...

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Abstract

The invention relates to bursa of Fabricius heptapeptide with immune regulation effect and application thereof in immunity (in improving the immune capability of animals, improving the immune effect of vaccines and affecting the activity of tumor cells), and belongs to the field of immunology. The molecular weight of the separated heptapeptide is 722.240, the amino acid sequence is EPASGMM, and the heptapeptide has a simple structure, no toxic or side effect and extremely weak immunogenic property. The heptapeptide can be separated and extracted from bursa of Fabricius, also can be chemically synthesized, has low cost, and can be massively produced. The bursa of Fabricius heptapeptide has induction effect on the production of antibody and subtype thereof, and meanwhile can regulate the production of cell factors, partition of T lymphocytes and proliferation of spleen cells, and promote the immune reaction of the cells. The bursa of Fabricius heptapeptide is an immune regulation factoron functions, has wide application prospect on the aspects of immune regulation, immune therapy and the like, and can be applied in the fields of basic immune research, clinical application research and the like.

Description

1. Technical field [0001] The invention relates to a bursal heptapeptide with immunoregulatory effect and its application in immunity (improving the immune ability of animals, improving the immune effect of vaccines and affecting the activity of tumor cells), belonging to the field of immunology. 2. Background technology [0002] The bursa of Fabricus (BF) was first discovered by Hieronymus Fabricus in 1961. At the same time, the shape and location of the supraluminal bursa were clarified, so it was named bursa of Fabricus. The bursa of Fabricius is the central lymphoid organ of humoral immunity unique to poultry. It is located on the dorsal side of the cloaca. Its blind end is sac-shaped and faces the front of the body. Early researchers observed that the bursa gradually degenerates with sexual maturity, so it has always been considered an organ related to reproductive physiology. Since 1956, when Glick and others discovered that removing the bursa of Fabricius would reduc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20A61K39/39
Inventor 冯秀丽陈溥言王芳权魏建超曹瑞兵周斌茅翔
Owner NANJING AGRICULTURAL UNIVERSITY
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