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Method and biochemical treatment agent for preventing and controlling blue-green algae

A biochemical treatment and cyanobacteria technology, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problem of easily breaking the ecological balance of water bodies, interfering with the safety of aquatic animals, and difficult to prevent and control effects. Willingness and other issues to achieve the effect of good applicability and stability, high degree of water eutrophication, and reasonable cost

Active Publication Date: 2010-09-15
NANJING SHENKELONG ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical agents themselves increase the total amount of pollutants in the water body, resulting in secondary pollution, and easily break the original ecological balance of the water body and interfere with the safety of aquatic animals
Therefore, with the development of industry and agriculture, climate warming and the increase of extreme weather, the problem of blue-green algae outbreak will become more serious, and the control effect is difficult to achieve as expected

Method used

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  • Method and biochemical treatment agent for preventing and controlling blue-green algae
  • Method and biochemical treatment agent for preventing and controlling blue-green algae
  • Method and biochemical treatment agent for preventing and controlling blue-green algae

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preparation example Construction

[0042] (3) Preparation of the above-mentioned algae-removing water purifying agent L agent: by the compound of sodium percarbonate, the 4th generation polyamide-amine, sodium bentonite, its weight ratio is sodium percarbonate: the 4th generation polyamide-amine : Sodium bentonite is 40%: 50%: 10%. The sodium bentonite requires less than 8% water content and 300 meshes.

[0043] The 4th generation polyamide-amine involved in the algaecide water purifying agent L agent of the present invention can be bought in the market, and can also be prepared. Polyamide-amine (PAMAM) dendrimers are a relatively mature class of research in dendrimer chemistry at present, and have been commercialized. There are many related technical reports and documents, for example, in January 2010, "Zhonghua Paper Industry", Volume 31, No. The second phase of "Research on the application of polyamide-amine dendrimers in the treatment of papermaking wastewater" introduced the preparation method of the four...

Embodiment 1

[0052] At first prepare three kinds of treatment agents S, K, L that the present invention relates to:

[0053] Preparation of cyanophage S agent: Weigh 1kg spirulina powder (edible grade refined pure powder), 0.1kg

[0054] NaCl (industrial grade pure powder) and an appropriate amount of pure water are prepared into a culture solution with a concentration of 1.1% by weight. After being sterilized in a sterilizer at 121°C for 30 minutes, add to a biological fermentation tank, and then add 3.5L of Bdellovibrio 3 ×10 7 pfu / bacteria liquid, fermented at 28°C for 72 hours to obtain algae phage S agent.

[0055] Biological bottom modifier K agent: take 40L respectively, containing 3×10 bacteria 7 cfu / yeast liquid, 20L contains 2.3×10 7 cfu / of Bacillus subtilis liquid and the above-mentioned 40L bacteria content 5×10 7 pfu / of the bacteriophage Bdellovibrio fluid. Put them together into the mixing tank, mix and stir to obtain the biological bottom modifier K agent bacterial li...

Embodiment 1-1

[0059] Taihu Lake water and sediment were taken, and various blue-green algae such as Anabaena, Spirulina, and Seaweed were detected and analyzed. Fill the Taihu Lake water and mud into six 1m 3 water tank, and each group was inoculated with Spirulina 1×10 3 A / ml, supplement nutrient sources, add nitrogen fertilizer and phosphorus fertilizer 1‰, simulate the water system of Taihu Lake. 1 as the control group and 5 as the test group.

[0060] 1. Usage in spring:

[0061] When the temperature in spring is 16°C, start to sprinkle algae-bacteria S agent bacteria solution 1 / m on the water surface of the test 5 groups of tanks 3 , use biological bottom modifier K agent 1 / m 3 Mix with 3g of solidified carrier powder to absorb moisture and sprinkle water.

[0062] In order to compare the application effect, another 1ml / m of bacteria liquid of algae phage S was splashed in the test group 4 3 . After 20 days, the water body in the control group, test group 1, test group 2, and te...

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Abstract

The invention relates to a method and treatment agent for preventing and controlling blue-green algae. A cyanophage bacterium S agent is a culture solution with the solid content of 0.5-2 percent, which is prepared from spirulina powder, NaCl and purified water; a biological substrate modified agent of K agent is prepared by compounding saccharomycetes, bacillus subtilis and cyanophage bdellovibro; and an algal removing water purifying agent of L agent is prepared from 40wt% of sodium carbonate, 50wt% of fourth generation of polyamidoamine and 10 wt% of sodium bentonite. The invention overcomes the defects of two methods by using beneficial bacterium which scrambles nutrition with the blue-green algae and cyanophage capable of phagocytosing the blue-green algae. The invention is a system engineering for preventing and controlling blue-green algae, which organically combines a biological method and a chemical method and can prevent and control blue-green algae comprehensively with proper cost and favorable practicability and stability; and the blue-green algae is controlled by using a microbial method at early stage with obvious implementation effect , since a cell wall of the blue-green algae is thinner and cyanophage can phagocytose algae quickly; in addition, since a water body has high eutrophication degree in summer, comprehensive treatment can achieve good effect.

Description

technical field [0001] The invention relates to a method and treatment agent for preventing and treating cyanobacteria, in particular to a method for preventing and controlling cyanobacteria and biochemical treatment agents S, K and L suitable for lakes, landscapes and aquaculture water bodies. Background technique [0002] Cyanobacteria have the dual nature of plant and bacteria. It has a thick layer of colloid outside the cell, which can resist the cold and heat stimulation of the outside world. It can exist in the high temperature of 90 ℃, and it can also exist in the freeze. In winter, the algae are "false" "It freezes to death, and when the water body decreases, it still survives in the underwater mud. In warm spring, when the temperature is above 18°C, it begins to grow in the water body, and it is more likely to grow wildly in summer. [0003] Controlling blue-green algae is a complex system engineering. The main method adopted at present is to invest a lot of manpow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 田宝凤冯忠静赵光勇田伟汉
Owner NANJING SHENKELONG ENVIRONMENTAL PROTECTION
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