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Recombinant spodoptera exigua nucleopolyhrdrovirus preparation and preparation method thereof

A technology of nuclear polyhedrosis virus and beet armyworm, applied in botany equipment and methods, insecticides, biocides, etc., can solve problems such as unfavorable virus diffusion, facilitate virus diffusion, facilitate field promotion and application, and improve killing The effect of pest efficiency

Inactive Publication Date: 2010-09-15
ZHAOQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, it is not conducive to the spread of the virus to the environment, which is a drawback of the current recombinant baculovirus as a biopesticide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Preparation of recombinant beet armyworm nuclear polyhedrosis virus preparation according to the present invention

[0022] 1. Source and preparation of raw materials

[0023] Recombinant beet armyworm nuclear polyhedrosis virus and wild-type beet armyworm nuclear polyhedrosis virus were obtained from the State Key Laboratory of Pest Control and Resource Utilization, Sun Yat-sen University. The laboratory is supported by Sun Yat-sen University, which passed the national inspection and acceptance in 1995 and was officially opened to domestic and foreign. room".

[0024] For the wild-type beet armyworm nuclear polyhedrosis virus of the present invention, please refer to Lv Hongsheng, editor-in-chief. Insect virus and insect virus disease [M]. Beijing: Science Press, 1982.

[0025] For recombinant beet armyworm nuclear polyhedrosis virus of the present invention, see Xiaojiang Dai, József P.Hajós3, Nina N.Joosten, Monique M.van Oers, Wilfred F.J.IJkel, Douw...

Embodiment 2

[0033]Embodiment two: the laboratory effect of recombinant beet armyworm nuclear polyhedrosis virus preparation of the present invention

[0034] Optionally select the preparation described in embodiment one, indoor by every gram of feed coating 1 * 10 6 The standard of a virus polyhedron is evenly applied to the surface of the feed and fed to 1-3 instar insect larvae.

[0035] The results show that the insecticidal rate of the insecticidal preparation of the present invention is 20% higher than that of the wild-type virus, and compared with the simple recombinant baculovirus preparation, the insecticidal effect has no statistical difference (p<0.05). This result shows that the organic mixture of the recombinant virus and the wild-type virus can not only realize the liquefaction of the host after infection of the recombinant virus, but also facilitate the spread of the virus, while maintaining the high virulence of the recombinant virus and improving the control of the virus i...

Embodiment 3

[0036] Embodiment three: field test effect of recombinant beet armyworm nuclear polyhedrosis virus preparation of the present invention

[0037] The preparation described in Example 1 is arbitrarily selected, and sprayed with common equipment such as a manual sprayer or a misting machine. When insect larvae in the 1-3 instar stage were sprayed with pesticides, it was found that the infected larvae gradually showed loss of appetite and sluggish movements. After 3 days, they stopped feeding, and the color of the body wall became pale. Most insects behave abnormally. The larvae hold the branches tightly with their hips and feet, and the whole body hangs upside down on the plant. At this time, the tissues in the body liquefy, and the body fluids fall down to make the front of the body swell, and they hang to death. Generally, the whole process from infection to death is 3 to 6 days. The dead larvae were enlarged and liquefied obviously. Compared with the wild-type virus, the ins...

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Abstract

The invention relates to an improved biological insecticide and a preparation process thereof. The recombinant spodoptera exigua nucleopolyhrdrovirus preparation comprises the following components in percentage by weight: 1-10 percent of mixed nucleopolyhrdrovirus suspension, 74-94.5 percent of carrier, 1-4 percent of dispersing agent, 1-4 percent of wetting agent, 0.5-4 percent of photoprotectant and 1-4 percent of speader, wherein the mixed nucleopolyhrdrovirus suspension comprises 50 billion PIB (Polyhedral Inclusion Body) / g of recombinant spodoptera exigua nucleopolyhrdrovirus suspension and 50 billion PIB / g of wild type spodoptera exigua nucleopolyhrdrovirus suspension which are mixed according to a weight ratio of 3:1. The virus preparation is used for infecting host insects, which not only maintains the characteristic of high disinfestation efficiency of the recombinant viruses, but also can ensure that the dead host infected by the recombinant viruses is liquefied so as to be beneficial to the epidemic spread of the recombinant viruses in the environment, thereby the problem of poor continuous control effect on target pests when the recombinant viruses deleting egt genes are singly used is effectively solved.

Description

technical field [0001] The invention belongs to the field of biopesticides, and relates to an improved biopesticide and a related preparation process. Background technique [0002] Insect viruses are a class of viruses that host insects and can cause epidemics in their populations. Compared with the traditional chemical insecticides used in large quantities, virus insecticides with insect viruses as active ingredients have the following characteristics: high control efficiency, not only the dosage is extremely low, but also the effect of continuous control can be achieved; Non-target animals are safe and protect natural enemies; greatly reduce the chemical residues in the environment and food; increase the biodiversity of the application environment; the dosage form is convenient for storage and transportation, and has a long shelf life; it is suitable for conventional application methods and integrated with pests The control system has good compatibility and is showing inc...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P7/04
Inventor 李充璧李广宏李赛男李芸瑛
Owner ZHAOQING UNIV
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