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Process for separating acetone, butanol and ethanol in situ by coupling biomass fermentation and pervaporation

A biomass fermentation and pervaporation technology, which is applied in the field of in-situ separation of acetone, butanol and ethanol by coupling pervaporation and biomass fermentation, can solve the problem that the product is difficult to achieve high purity requirements, affects the total solvent yield of ABE, and weakens enzyme activity. and other problems to achieve the effect of reducing the probability of bacterial contamination, shortening the production cycle, and eliminating the inhibitory effect.

Active Publication Date: 2010-08-18
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the fermentation process, the accumulation of a large amount of metabolites ABE (acetone, butanol, ethanol) in the fermentation process produces serious inhibitory effect on the fermentation process, the activity of the enzyme is weakened, and the thalline is dead, so it has a strong impact on the production of the total solvent of ABE. Rate
In addition, for the general ABE batch fermentation process, the mass concentration of ABE in the fermenter will not exceed 2%. This low-concentration ABE aqueous solution is concentrated by traditional rectification, which requires high energy consumption and the product is difficult to meet high purity requirements. , making it impossible to compete with the traditional petroleum method to prepare butanol

Method used

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  • Process for separating acetone, butanol and ethanol in situ by coupling biomass fermentation and pervaporation
  • Process for separating acetone, butanol and ethanol in situ by coupling biomass fermentation and pervaporation
  • Process for separating acetone, butanol and ethanol in situ by coupling biomass fermentation and pervaporation

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Embodiment 1

[0020] use figure 1 The process flow chart of scheme one shown.

[0021] The corn stalk sugar solution obtained after a series of pretreatments was used as a fermentation substrate to prepare a medium, and the medium composition was: total sugar concentration 60g / L, corn steep liquor 2g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, FeSO 4 0.01g / L. Open the feed valve 7, inject the sterilized medium into the high-temperature sterilized fermenter 1, inoculate Clostridium beijerinckii, carry out ABE fermentation at 37°C for 36 hours, and detect the quality of the total ABE solvent in the fermenter The concentration reaches 1.0%. Now open the discharge valve 8, the feed pump 5 and the vacuum pump 6, and the fermented liquid enters the pervaporation membrane separator 2 with a feed rate of 6L / h, and the vacuum on the permeate side is maintained at 3 mm Hg, and the pervaporation The separator is made of a unit pervaporation membrane separator, and the pervaporation membrane adopted ...

Embodiment 2

[0024] use figure 2 The process flow chart of scheme two shown.

[0025] Corn flour was used as a fermentation substrate to prepare a medium, and the medium composition was as follows: the concentration of corn flour was 60g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L. Open the feed valve 7, inject the sterilized culture medium into the high-temperature sterilized fermenter 1, inoculate Clostridium acetobutylicum at the same time, carry out ABE fermentation at 35°C for 24 hours, and detect the quality of the total ABE solvent in the fermenter The concentration reaches 0.5%. Now open the discharge valve 8, the feed pump 5, the fermented liquid with high solid content in the fermenter is passed into the aluminum alloy with an average pore diameter of 0.5 μm. 2 o 3The microfiltration membrane separator of the microfiltration membrane, the intercepted feed liquid first enters the preheater 4 to exchange heat with the fresh corn flour medium, and then returns to the fermenter 1, ...

Embodiment 3

[0028] use figure 1 The process flow chart of scheme one shown.

[0029] Glucose is used as a fermentation substrate to prepare a medium, and the medium composition is: the total sugar concentration is 70g / L, yeast powder is 1g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, MnSO 4 ·H 2 O0.01g / L. Open the feed valve 7, inject the sterilized culture medium into the high-temperature sterilized fermenter 1, inoculate Clostridium acetobutylicum, carry out ABE fermentation at 36°C for 48 hours, and detect the quality of the total ABE solvent in the fermenter The concentration reaches 1.2%. Now open the control valve 8, the feed pump 5 and the vacuum pump 6, the fermented liquid enters the pervaporation separator 2 with a feed rate of 6L / h, the vacuum on the permeate side is maintained at 2 mm Hg, and the pervaporation separator It is composed of a unit pervaporation membrane separator, and the pervaporation membrane used in the pervaporation membrane separa...

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Abstract

The invention relates to a process for separating acetone, butanol and ethanol in situ by coupling biomass fermentation and pervaporation. A pervaporation separation process is coupled with a fermentation process, and ABE (acetone, butanol and ethanol) solvents produced by fermentation are removed in situ and in time through a pervaporation film, so inhibiting effects of the ABE solvents produced in the fermentation process on the growth of microbial cells are greatly reduced and production intensity and efficiency of ABE fermentation are enhanced. Simultaneously, compared with an ordinary rectification process, the process also greatly lowers energy consumption for separating the ABE solvents produced by biomass fermentation, shortens production time and greatly lowers production cost of fuel butanol.

Description

technical field [0001] The invention relates to a process for in-situ separation of acetone, butanol and ethanol from metabolites ABE in a fermentation process, in particular to a process for in-situ separation of acetone, butanol and ethanol by coupling pervaporation and biomass fermentation. Background technique [0002] In the past two decades, due to the shortage and depletion of chemical energy sources, butanol, a biomass fuel prepared by fermentation of biomass, is considered to be a promising renewable energy source. But in the fermentation process, the accumulation of a large amount of metabolites ABE (acetone, butanol, ethanol) in the fermentation process has a serious inhibitory effect on the fermentation process, the activity of the enzyme is weakened, and the thalline is dead, so it seriously affects the production of the total solvent of ABE. Rate. In addition, for the general ABE batch fermentation process, the mass concentration of ABE in the fermenter will n...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P7/16C12P7/36C07C31/12C07C31/08C07C29/76C07C49/08C07C45/78C12R1/145
CPCY02E50/16Y02E50/10
Inventor 金万勤姜岷刘公平吴昊
Owner NANJING UNIV OF TECH
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