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Method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant

A technology of edible fungus and biological surface, which is applied in the field of remediation of polluted environment, can solve the problems of limited efficiency and low bioavailability, so as to increase the quantity and metabolic activity, improve bioavailability and degradation rate, and improve bioavailability. Effects of Utilization and Degradability

Inactive Publication Date: 2010-07-21
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low bioavailability of polycyclic aromatic hydrocarbons in soil, the efficiency of phytoremediation is often limited in the short term. Usually, the degradation rate of PAHs in a complete plant growth cycle is only 10% to 20%.

Method used

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  • Method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant
  • Method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant
  • Method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: Containing the polluted soil of polycyclic aromatic hydrocarbons total amount 10.31mg / kg, alfalfa is planted in hole planting mode, planting hole spacing is 40cm, after the plant survives, manage according to the management method of common crops; wait for the plant to grow to plant height At 10cm, apply 0g, 5g, 10g and 20g of Agaricus bisporus chaff to the soil respectively. At the same time, apply 0.5L of rhamnolipid fermentation liquid with a concentration of 3g / L to the soil respectively, mix well and cover with soil about 2cm. Collect soil samples and analyze changes in the content of polycyclic aromatic hydrocarbons in the soil to evaluate the remediation effect.

[0022] After the soil is collected, pass through a 100-mesh sieve, weigh 1g of soil and add 2g of anhydrous Na 2 SO 4 , mix well, add 60ml of dichloromethane, Soxhlet extraction for 24h, evaporate to dryness with a rotary evaporator, then use 2ml of cyclohexane to fix the volume, take 0.5...

Embodiment 2

[0025] In the polluted soil containing 8.87mg / kg total amount of polycyclic aromatic hydrocarbons, alfalfa was planted by hole planting, with a planting hole spacing of 35cm. After the plants survived, they were managed according to the management methods of ordinary crops; Apply 10g of Pleurotus eryngii mushroom chaff to the soil, and at the same time, apply 0.25L, 0.5L, 0.75L and 1L of rhamnolipid fermentation broth with a concentration of 4g / L to the soil, mix well and cover with soil about 2cm, and collect the soil after 120 days of cultivation Samples were collected, and changes in the content of polycyclic aromatic hydrocarbons in the soil were analyzed to evaluate the remediation effect.

[0026] After the soil is collected, pass through a 100-mesh sieve, weigh 1g of soil and add 2g of anhydrous Na 2 SO 4 , mix well, add 60ml of dichloromethane, Soxhlet extraction for 24 hours, evaporate to dryness with a rotary evaporator, then use 2ml of cyclohexane to fix the volume...

Embodiment 3

[0029] In the polluted soil containing 13.27mg / kg total amount of polycyclic aromatic hydrocarbons, alfalfa was planted by hole planting, with a planting hole spacing of 40cm. After the plants survived, they were managed according to the management methods of ordinary crops; Apply 10g of Pleurotus ostreatus chaff to the soil. At the same time, apply 0.25L, 0.5L and 0.75L of rhamnolipid fermentation broth with a concentration of 4g / L to the soil. After mixing evenly, cover the soil with about 2cm. And the control of applying different doses of rhamnolipid fermentation broth. Soil samples were collected after 120 days of cultivation, and the changes in the content of polycyclic aromatic hydrocarbons in the soil were analyzed to evaluate the restoration effect.

[0030] After 120 days, the degradation rate data of the total amount of polycyclic aromatic hydrocarbons in soil can be seen in image 3 shown. Wherein, the degradation rate of no plant control is only 2.6%, the degrad...

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Abstract

The invention discloses a method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant, which comprises the following steps: (1) planting alfalfa in the polycyclic aromatic hydrocarbon-polluted soil at intervals of between 30 and 40cm; (2) when the height of the plant is between 7 and 10cm, applying 10 to 20g of edible fungus residue in dry weight to the soil in the depth of between 5 and 10cm within a range that the diameter of plant root circles is between 5 and 25cm; (3) applying 1 to 3g of biosurfactant in a deep soil layer with 5 to 10 cm, stirring uniformly and covering earth by 3 to 5cm; (4) repeating the step (3) every 60 days; and (5) harvesting the plant bodies of the alfalfa after 120 days, drying in the sun, and transporting to other places for burning. The method can effectively promote PAHs organic pollutants to desorb from soil particles, and improve the bioavailability and degradation rate of the organic pollutants without secondary pollution; and the cost is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of remediation of polluted environments, in particular to a method for remediating polycyclic aromatic hydrocarbon-contaminated soil by using edible fungus chaff and biosurfactants to strengthen phytoremediation. Background technique [0002] Soil is one of the most important natural resources for human beings to survive. However, soil organic pollution is becoming more and more serious due to atmospheric deposition, sewage irrigation, solid waste landfill leakage and oil field exploitation. Polycyclic aromatic hydrocarbons are a class of organic pollutants containing two or more benzene rings, which have carcinogenic, teratogenic and mutagenic effects, can migrate in the soil-crop system, and cause secondary pollution of groundwater, endangering the safety and security of agricultural products. human health. Soil PAHs pollution not only affects the health of the soil ecosystem, but also affects human healt...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
Inventor 张晶林先贵尹睿
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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