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Novel method for preparing high-purity chlorogenic acid

A chlorogenic acid, high-purity technology, used in the preparation of carboxylate, chemical instruments and methods, preparation of organic compounds, etc. Adsorption and extraction, continuous and simple operation, and the effect of improving purity

Inactive Publication Date: 2009-08-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Through the combination of the above two patented technical solutions, the combination technology of multi-column series adsorption and continuous countercurrent extraction can effectively solve many shortcomings such as low preparation purity, small preparation amount, complicated preparation process and non-continuous preparation operation.

Method used

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  • Novel method for preparing high-purity chlorogenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The water extract of honeysuckle after deoiling with a pH value of 1 and a concentration of chlorogenic acid of 0.5g / L was used as a raw material, and a 4-stage multi-column series device was selected, and the filler was non-polar adsorption resin HPD-100A (Cangzhou Baoen Adsorption Material Science and Technology Co., Ltd.), the washing solution is hydrochloric acid aqueous solution with pH 3, the eluent is 10% ethanol-water solution, and the regeneration solution is absolute ethanol. Pump the raw material into the first-stage adsorption column at a flow rate of 1BV / h, switch to the second-stage adsorption column for adsorption at the same flow rate of the adsorption liquid after breakthrough, and press the flow rate of 5BV / h for the first-stage adsorption column after penetration. h, 1BV / h and 5BV / h are sequentially pumped into the washing liquid, eluent and regeneration liquid. After the second-stage column is adsorbed and penetrated, it is switched, and the impurity...

Embodiment 2

[0054] The water extract of Eucommia ulmoides after deoiling with a pH value of 4 and a chlorogenic acid concentration of 10 g / L was used as a raw material, a 64-stage multi-column series device was selected, and the filler was medium polarity adsorption resin AB-8 (Cangzhou Baoen Adsorption Material Technology Co., Ltd. Co., Ltd.), the washing liquid is hydrochloric acid aqueous solution of pH 3, the eluent is 50% ethanol, and the regeneration liquid is absolute ethanol. Pump the raw material into the first-stage adsorption column at a flow rate of 5BV / h, switch to the second-stage adsorption column for adsorption at the same flow rate of the adsorption liquid after breakthrough, and press the flow rate of 10BV / h for the first-stage adsorption column after penetration. h, 5BV / h and 10BV / h are sequentially pumped into the washing liquid, eluent and regeneration liquid. After the second-stage column is adsorbed and penetrated, it is switched, and the impurity washing, elution a...

Embodiment 3

[0057] The deoiled water extract of coffee beans with a pH value of 2 and a chlorogenic acid concentration of 1 g / L was used as raw material, a 32-stage multi-column series device was used, and the filler was polar adsorption resin HPD-600 (Cangzhou Baoen Adsorption Material Technology Co., Ltd. Co., Ltd.), the washing solution is hydrochloric acid aqueous solution with pH3, the eluent is 20% ethanol, and the regeneration solution is absolute ethanol. Pump the raw material into the first-stage adsorption column at a flow rate of 1BV / h, switch to the second-stage adsorption column for adsorption at the same flow rate of the adsorbent after breakthrough, and press the flow rate of 8BV / h for the first-stage adsorption column after penetration. h, 2BV / h and 8BV / h are sequentially pumped into the washing liquid, eluent and regeneration liquid. After the second-stage column is adsorbed and penetrated, it is switched, and the impurity washing, elution and regeneration are carried out...

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Abstract

The invention provides a new method for preparing high purity chlorogenic acid, comprising the steps: after the stock solution of the chlorogenic acid is processed by washing impurity and eluting through at least four levels of macroporous resin series connection adsorption column, and then the eluate is regenerated for continuous countercurrent extraction, so that the high purity chlorogenic acid is obtained. The method is applicable to light concentration stock solution of the chlorogenic acid, and can complete the process of absorbing, washing impurity, eluting, regenerating, countercurrent extracting and recycling the solvent in a semicontinuous way; the handling capacity of samples is large, the operation is simple, the production cost can be lowered, the residual organic solvent is reduced, and the environmental pollution is relieved.

Description

technical field [0001] The invention relates to a new method for preparing high-purity chlorogenic acid, specifically a method for preparing high-purity chlorogenic acid by combining multi-column series adsorption and continuous countercurrent extraction, belonging to the field of natural product purification. Background technique [0002] Chlorogenic acid is a kind of polyphenolic compound, which is a kind of biologically active substance widely existing in nature. The main ingredient of traditional Chinese medicine for clearing heat, detoxifying, anti-inflammatory and antibacterial. Studies have shown that chlorogenic acid has pharmacological effects such as antibacterial, antiviral, antioxidative, free radical scavenging, immune regulation, antitumor, and antilipidemia. Therefore, chlorogenic acid is more and more widely used in the fields of biochemical reagents, pharmaceutical intermediates and preparations, and is currently internationally recognized as "plant gold". ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56C07C67/58A61P31/00A61P31/12A61P39/06A61P37/02A61P35/00A61P3/06
Inventor 卢定强赵辉王俊凌岫泉蒋奔
Owner NANJING UNIV OF TECH
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