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Three-dimensional porous nano-bracket based on fibrinogen and preparation method thereof

A fibrinogen, three-dimensional porous technology, applied in fiber processing, medical science, filament/thread forming, etc., to achieve the effect of easy secretion, favorable growth and migration, and good interstitial connectivity

Active Publication Date: 2010-07-21
SHANGHAI P & P BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report in the existing literature on the production of three-dimensional porous nanoscaffolds based on fibrinogen by electrospinning

Method used

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  • Three-dimensional porous nano-bracket based on fibrinogen and preparation method thereof
  • Three-dimensional porous nano-bracket based on fibrinogen and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0028] The first step: dissolving fibrinogen in a solvent mixed with hexafluoroisopropanol and 10×DMEM medium at a volume ratio of 9:1 to prepare a fibrin stock solution with a mass fraction of 100 mg / ml;

[0029] The second step: dissolving polylactic acid / polycaprolactone copolymer (the weight ratio of polylactic acid / polycaprolactone is 7: 3, molecular weight is 150,000) in hexafluoroisopropanol to prepare mass fraction is 8% Polylactic acid / polycaprolactone stock solution;

[0030] Step 3: Mix the fibrin stock solution with the polylactic acid / polycaprolactone stock solution at a volume ratio of 1:4, then add it to the same capacity tube of the electrospinning machine for electrospinning, and the spinning voltage is 2kv / spinning distance cm, spinning distance 12cm, spinning solution propulsion speed 3ml / h, obtain a diameter of 0.5cm on the receiving device (a steel pipe with a rotating speed of 500rpm and an outer diameter of about 0.5cm), and a length of a tubular three-d...

Embodiment 2

[0033] The first step: dissolving fibrinogen in a solvent mixed with hexafluoroisopropanol and 10×DMEM medium at a volume ratio of 9:1 to prepare a fibrin stock solution with a mass fraction of 80 mg / ml;

[0034] The second step: the polylactic acid / polycaprolactone copolymer (the weight ratio of polylactic acid / polycaprolactone is 5: 5, and molecular weight is 50,000) is dissolved in chloroform and is the polylactic acid of 5% by mass fraction / Polycaprolactone stock solution;

[0035]Step 3: Mix the fibrin stock solution with the polylactic acid / polycaprolactone stock solution at a volume ratio of 1:2, then add it to the same capacity tube of the electrospinning machine for electrospinning, and the spinning voltage is 0.5kv / spinning Distance cm, spinning distance 12cm, spinning solution propulsion speed 0.5ml / h, obtain a diameter of 0.5cm on the receiving device (a steel pipe with a rotating speed of 500rpm and an outer diameter of about 0.5cm), and a length of 10cm tubular ...

Embodiment 3

[0038] The first step: dissolving fibrinogen in a solvent mixed with hexafluoroisopropanol and 10×DMEM medium at a volume ratio of 9:1 to prepare a fibrin stock solution with a mass fraction of 120 mg / ml;

[0039] The second step: dissolving polylactic acid / polycaprolactone copolymer (the weight ratio of polylactic acid / polycaprolactone is 8: 2, molecular weight is 300,000) is dissolved in dimethylformamide and is 10% by mass fraction Polylactic acid / polycaprolactone stock solution;

[0040] Step 3: Mix the fibrin stock solution with the polylactic acid / polycaprolactone stock solution at a volume ratio of 1:1 and then add it to the same capacity tube of the electrospinning machine for electrospinning. The spinning voltage is 3kv / spinning distance cm, the spinning distance is 12cm, the spinning solution propulsion speed is 4ml / h, and a film-like three-dimensional porous nano-stent of 5cm×10cm is obtained on the receiving device (5cm×10cm flat steel plate);

[0041] The fourth ...

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Abstract

The invention provides a three-dimensional porous nano-bracket based on fibrinogen and a preparation method thereof. The three-dimensional porous nano-bracket based on the fibrinogen is characterized by being prepared by the fibrinogen and polylactic acid / polycaprolactone copolymer. The preparation method of the three-dimensional porous nano-bracket comprises the steps of: preparing fibrinogen stock solution and polylactic acid / polycaprolactone stock solution; electrostatic spinning; and post-treatment. The invention has the characteristics of good specific surface area, high porosity, good gap connectivity and the like.

Description

technical field [0001] The invention relates to a three-dimensional porous nano scaffold based on fibrinogen and a preparation method thereof, belonging to the field of medical tissue engineering. Background technique [0002] Tissue engineering is a new interdisciplinary subject proposed in the 1980s. It mainly uses the principles of biological science and engineering to develop a science for repairing, reconstructing and maintaining the structure and function of damaged tissues and organs. Tissue engineering research mainly focuses on the construction of three-dimensional scaffolds and the selection of seed cells. An ideal tissue engineering scaffold should have the following characteristics: (1) Good biocompatibility, surface microstructure and biological characteristics are conducive to the adhesion and proliferation of seed cells; (2) Appropriate mechanical properties, in line with the mechanical properties of tissues and organs at the replacement site Performance; (3)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/22A61L27/18A61L27/56D01D5/00
Inventor 符伟国方征东董智慧张祥满郭大乔何红兵王玉琦
Owner SHANGHAI P & P BIOTECH
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