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Homocysteine diagnosis/determination reagent (kit) and homocysteine concentration determination method

A technology for homocysteine ​​and concentration determination, which is applied in biological testing, biochemical equipment and methods, microbial measurement/inspection, etc. It can solve the problems of long test time, complicated operation, and large variation of test data.

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0044] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0045]Stabilizer 500mmol / L

[0046] Reduced coenzyme 0.25mmol / L

[0047] Cystathionine β synthase 6000U / L

[0048] Cystathionine β-lyase 8000U / L

[0049] Lactate dehydrogenase 10000U / L

[0050] Serine 3mmol / L

[0051] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0052] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested homocysteine ​​sample to the reagent is 1 / 25, the reaction direction is negative reaction (falling reaction), the delay time is about 2 minutes, and the detection time is about 2 min...

Embodiment 2

[0055] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0056] Reagent 1

[0057] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0058] Stabilizer 50mmol / L

[0059] Reduced coenzyme 0.25mmol / L

[0060] Serine 3mmol / L

[0061] Reagent 2

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 500mmol / L

[0064] Cystathionine β synthase 6000U / L

[0065] Cystathionine β-lyase 8000U / L

[0066] Lactate dehydrogenase 10000U / L

[0067] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0068] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, tested homocysteine ​​sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction is ne...

Embodiment 3

[0071] The homocysteine ​​diagnosis / measurement reagents of this embodiment are three reagents, including:

[0072] Reagent 1

[0073] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0074] Stabilizer 50mmol / L

[0075] Reduced coenzyme 0.25mmol / L

[0076] Serine 3mmol / L

[0077] Reagent 2

[0078] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0079] Stabilizer 500mmol / L

[0080] Cystathionine β-lyase 8000U / L

[0081] Lactate dehydrogenase 10000U / L

[0082] Reagent 3

[0083] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0084] Stabilizer 500mmol / L

[0085] Cystathionine β synthase 6000U / L

[0086] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0087] When measuring the concentration of homocysteine, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance...

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Abstract

The invention relates to a homocysteine diagnosis / determination reagent (kit) using enzyme-colorimetry and enzyme-linked method technology. Meanwhile, the invention also relates to a homocysteine concentration determination method, reagent compositions and components, which belong to the technical filed of medical test and determination. The reagent (kit) of the invention mainly includes: buffer solution, reduced coenzyme, serine, cystathionine Beta synthase, cystathionine Beta catenase, lactic dehydrogenase and stabilizing agent; samples and reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions; then the reactants are arranged under an ultraviolet / visible light analyzer to detect the decreasing degree of absorbance at the 340nm position of a dominant wave so as to measure and calculate the concentration of the homocysteine.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnosis / measurement reagent (box), and at the same time, the invention also relates to a method for measuring the homocysteine ​​concentration, which belongs to the technical field of medical examination and measurement. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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