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Sephadex magnetic composite particles and preparation and use thereof

A technology of cross-linked dextran and composite particles, which is applied in the directions of magnetic materials, magnetic objects, drug combinations, etc., can solve the problems of large encapsulation capacity, poor magnetic properties of magnetic particles, and inability to obtain magnetic responsiveness, and achieves encapsulation capacity. Large, controllable in size, conducive to promotion

Active Publication Date: 2010-06-30
XIAN GOLDMAG NANOBIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Xia, Z.F. et al. (2005, 293, 182-186, Journal of Magnetism and Magnetic Materials) synthesized dextran-ferric oxide composite particles by ultrasonic coprecipitation method, and provided The influence of the amount of iron on magnetism also has certain reference significance for the present invention, but the magnetism of the obtained magnetic particles is also relatively poor
The preparation methods of dextran magnetic composite particles reported so far cannot obtain magnetic composite particles with strong magnetic response, large loading capacity, and sustained release of drugs.

Method used

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  • Sephadex magnetic composite particles and preparation and use thereof
  • Sephadex magnetic composite particles and preparation and use thereof
  • Sephadex magnetic composite particles and preparation and use thereof

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preparation example Construction

[0046] The chemical reaction formula of the preparation method of the cross-linked dextran magnetic composite particles is:

[0047]

[0048] The preparation method of the cross-linked dextran magnetic composite particles comprises the following steps

[0049] Step 1) Prepare dextran solution

[0050] Add ultrapure water and alkali solution to the dextran to prepare a dextran solution with a concentration ranging from 20 to 100 mg / ml.

[0051] Step 2) Synthesis of dextran magnetic composite particles

[0052] Add magnetic nanoparticles and alkali solution to the dextran solution obtained in the above step 1) to obtain a mixed system, and keep the alkali concentration in the system consistent with the alkali concentration of the dextran solution obtained in step 1), and the mixed system Stir while reacting to synthesize dextran magnetic composite particles.

[0053] Step 3) Synthesis of cross-linked dextran magnetic composite particles

[0054] Add an alkali solution to ...

specific Embodiment 1

[0071] Add 2g of dextran-40 into a 250ml round bottom flask, then add 10ml of ultrapure water and 10ml of 1M NaOH, and ultrasonically dissolve. Then add 400mg of magnetic nanoparticles (solid content about 20mg / ml) into a 250ml round bottom flask, add an equal volume of 1M NaOH, control the stirring speed at 400rpm, the reaction temperature at 26°C, and ultrasonically react for 4h. Add NaOH to adjust the alkali concentration in the system to 3M, and then add epichlorohydrin diluted 1:1 with isopropanol. The amount of epichlorohydrin is 24ml, and the constant pressure dropping funnel is used to complete the addition within 1h. The temperature was raised to 60°C, the stirring speed was controlled to 900rpm, and the reaction was continued for 12h. After the reaction is over, the system is neutralized by magnetic separation, centrifugation, etc., and cross-linked dextran magnetic composite particles are obtained. Its particle size is about 1-3 μm detected by laser particle size s...

specific Embodiment 2

[0072] Add 10ml of ultrapure water and 10ml of 1.3M NaOH, add 2g of dextran-20 into a 250ml round bottom flask, and ultrasonically dissolve. Then add 400mg of magnetic nanoparticles (solid content about 20mg / ml) into a 250ml round bottom flask, add an equal volume of 1.3M NaOH, control the stirring speed at 300rpm, the reaction temperature at 28°C, and ultrasonically react for 6h. Add NaOH to adjust the alkali concentration in the system to 2M. Then add epichlorohydrin diluted with isopropanol 2:1, the amount of epichlorohydrin is 48ml, and the constant pressure dropping funnel is used to complete the addition within 1.5h. The temperature was raised to 70°C, the stirring speed was controlled to 1200rpm, and the reaction was continued for 28h. After the reaction is over, the system is neutralized by magnetic separation, centrifugation, etc., and cross-linked dextran magnetic composite particles are obtained. Its particle size is about 1-3 μm detected by laser particle size sc...

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Abstract

The invention relates to sephadex magnetic composite particles and a preparation method and a use thereof, and the composite particles comprise magnetic nanoparticles and dextran with a cross-linked structure, wherein the magnetic nanoparticles are dispersed in the dextran with a cross-linked structure. The preparation method of the composite particles comprises the steps of preparation of dextran solution, synthesis of dextran magnetic composite particles and the synthesis of sephadex magnetic composite particles. The using steps of the composite particles are as follows: preparing the sephadex magnetic composite particles loading an anti-cancer drug and adding sustained release solution for drug release. The invention can solve the technical problems of weak magnetic responsiveness and small loading amount of the existing dextran magnetic composite particles, and the prepared sephadex magnetic composite particles are characterized by strong magnetic responsiveness, large drug loading amount, high concentration of the anti-cancer drug and capability of being acted on target cells through the positioning of a magnetic field and the sustained release role of the drug.

Description

technical field [0001] The invention relates to a magnetic permeation targeting carrier material and its preparation method and its use, in particular to a cross-linked dextran magnetic composite particle and its preparation method and its use. Background technique [0002] Magnetic polymer microspheres are a multifunctional material widely used in the field of biomedicine. This type of material not only has many characteristics of polymer particles, but also is endowed with magnetic characteristics, which can be rapidly separated from other components under the action of an external magnetic field, or oriented or moved by magnetic field induction. The required experimental conditions are simple, easy to operate, and low in cost, so it has broad application prospects in many fields such as cell separation, classification, immunoassay, immobilized enzymes, targeted drugs, DNA separation, and nucleic acid hybridization. These magnetic particles take iron oxide as the core, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08K3/22C08K3/24C08J3/24A61K47/36A61K45/00A61P35/00
CPCA61K9/0019A61K9/0009A61K9/1652H01F1/083A61K9/1611A61P35/00
Inventor 彭明丽刘艳红崔亚丽陈超李珂
Owner XIAN GOLDMAG NANOBIOTECH
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