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Extract of yangtao actinidia root and preparation method and applications thereof

A root extract and the technology of vine pear, applied in the field of vine pear root extract and its preparation, can solve the problems of high toxicity and instability

Inactive Publication Date: 2012-05-02
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are few fatty acid synthase inhibitors found so far, only cerulenin, C75, etc., and all have the disadvantages of instability and high toxicity

Method used

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  • Extract of yangtao actinidia root and preparation method and applications thereof
  • Extract of yangtao actinidia root and preparation method and applications thereof
  • Extract of yangtao actinidia root and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1, wisteria root extract

[0036] Grind 10 g of Tengli root (purchased in Tongrentang Pharmacy, Qianmen, Beijing), pass through a 800-mesh sieve, add 5 mL of extractant (ethanol) to each gram of Tengli root powder, and conduct ultrasonic extraction at room temperature for 1 h, with ultrasonic power at 600 W, during which it oscillates continuously , filter, collect the first ultrasonic extraction solution 1 and filter residue 1, repeat the above-mentioned extraction steps for all the filter residue 1 obtained twice, combine the extracts obtained three times, and put them under the conditions of 50°C and a vacuum of 0.02MPa Concentrate under reduced pressure, remove the solvent therein, and obtain the extract of vine root extract. The extract was dried (vacuum) to obtain 1.25 g of solid vine root extract.

[0037] The average extraction rate (extraction rate=mass of vine root extract / mass of vine root) of the three repeated experiments of t...

Embodiment 2

[0038] Embodiment 2, the detection of the vine pear root extract prepared by different extractants to the inhibitory activity of fatty acid synthase

[0039] Respectively with solvent water, volume percentage composition is 50% ethanol aqueous solution, volume percentage composition is 95% ethanol water solution, chloroform, ethyl acetate, sherwood oil is prepared according to the method for example 1 vine root extract, then each Take 8 μl of wisteria root extract to detect the inhibitory activity of the same amount of FAS in the same experimental system solution, and the results are shown in Table 1.

[0040] FAS total reactivity assay:

[0041] The activity was determined by spectrophotometry, with 5 μmol / L acetyl-CoA (Roche Diagnostics), 10 μmol / L malonyl-CoA (Roche Diagnostics), 35 μmol / L NADPH (Roche Diagnostics) as substrates, in Containing 1 mmol / L EDTA, 1 mmol / L DTT (dithiothreitol, Roche Diagnostics); 100 mmol / L KH at pH 7.0 2 PO 4 -K 2 HPO 4 Incubate at a constant...

Embodiment 3

[0045] The half-inhibitory concentration of embodiment 3, wisteria root extract

[0046] Measure mass concentration respectively and be 0ug / mL, 12.096ug / mL, 18.144ug / mL, 24.192ug / mL, 30.24ug / mL, 36.288ug / mL, the solution of the vine root ethanol extract of 97.0848ug / mL to FAS whole Inhibitory activity of the reaction. Contain the microgram number of vine pear root (actually contained in the solution is the extract of this microgram number vine pear root) with residual activity to every milliliter of measuring live solution, obtain residual activity and gradually reduce with the increase of vine pear root extract amount Curve. The half-inhibitory concentration was obtained from the point on the curve where the activity was half-inhibited. This parameter shows the strength of the fast binding inhibitory ability of the inhibitor, the smaller the value, the stronger the inhibitory ability, and it is usually used as a comparative index of inhibitory ability.

[0047] The results ...

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Abstract

The invention discloses an extract of yangtao actinidia root and a preparation method and applications thereof. The extract of yangtao actinidia root, which is provided by the invention, is prepared by the following method: using water and / or an organic solvent as an extractant to extract the extract of yangtao actinidia root from yangtao actinidia root. Experiment shows that the extract of yangtao actinidia root can effectively inhibit the activity of fatty acid synthase. The inhibition kinetic analysis shows that the inhibition of the extract of yangtao actinidia root for the fatty acid synthase and the substrates of acetylcoenzyme A and NADPH have a competitive and non-competitive mixed type relationship, but more close to the non-competitive relationship; and the inhibition of the extract of yangtao actinidia root for the fatty acid synthase and the substrate of malonyl coenzyme A have a competitive and non-competitive mixed type relationship. The extract of yangtao actinidia rootcan be applied to the preparation of weight-reducing medicament and the medicament for preventing and treating cancers, and also can be widely applied as food, health product and daily chemical additive.

Description

technical field [0001] The present invention relates to vine pear root extract and its preparation method and application. Background technique [0002] Tengli root, also known as kiwi root, is the dry root of Actinidia chinensis Planchl, a plant of the Actinidiaceae plant. It contains urso licacid, oleano licacid, succinic acid, dauco sterol and arginine, lysine, leucine, alanine, aspartate Amino acid, etc. and rich in Fe, Cu, Mn, Se and other elements. Tengli root is distributed in the south of the Yangtze River Basin. It has the functions of clearing heat and detoxifying, promoting blood circulation and dispelling stagnation, dispelling wind and dampness. It is used for rheumatoid arthritis, lymphatic tuberculosis, bruises, carbuncle and other diseases. [0003] Fatty acid synthase (EC.2.3.1.85, Fatty acid synthase, FAS) is the most important enzyme in the synthesis of endogenous fatty acids in higher animals. It is a macromolecular protein complex with 7 enzyme activit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P3/04A61P35/00A61K125/00
Inventor 李珺谢艳方赵光邹洪宋丰宇孙彬
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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