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Method for extracting transfer factors from cattle spleen

A transfer factor, spleen technology, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problems of long production cycle, high operating costs, difficult industrialized large-scale production, etc., to improve production. Efficiency, saving manpower and material resources, the effect of shortening the production cycle

Inactive Publication Date: 2010-06-30
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] At present, the existing transfer factor technology usually removes the fat and large blood vessels in the spleen of the animal, then smashes the tissue, freezes and thaws it repeatedly, and obtains it through semi-permeable membrane dialysis, which is expensive to operate
In addition, the Laurence method is routinely used, that is, the transfer factor is recovered by two dialysis methods. This method is simple to operate, but the production cycle is long and cumbersome (the production cycle takes about 20 days), which takes a long time and wastes a lot of manpower and material resources.
Therefore, these methods are difficult to carry out large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of transfer factor

[0042] 1. Cutting and cleaning: Remove the fat and blood vessels from the animal spleen that has thawed into a semi-frozen state, accurately weigh 1 kg, and wash 3 times with non-pyrogenic distilled water.

[0043] 2. Grinding: Weigh the degreased spleen and grind it three times in a meat grinder. Add 1000ml of pyrogen-free distilled water to the minced spleen, put it in a mashing tank, and finely grind it.

[0044] 3. Grinding: put the mashed spleen pulp into a colloid mill to grind it to make a homogenate.

[0045] 4. Cell disruption: Homogenize the above-mentioned spleen homogenate in a high-pressure homogenizer at a pressure of 60 MPa to make a homogenate.

[0046] 5. Add 2.0 g of citric acid to the high-pressure homogenized homogenate, stir evenly, place in a refrigerated centrifuge at 7,000 rpm, and centrifuge for 15 minutes at a centrifuge ambient temperature of 4°C. Discard the sediment and collect the supernat...

Embodiment 2

[0048] Embodiment 2: detection of transfer factor prepared by process of the present invention

[0049] The transfer factor stoste prepared by the process described in Example 1 is detected as follows according to the State Drug Administration and the National Drug Standard WS1-XG-036-2000:

[0050] 1. The appearance is a light yellow clear liquid, in line with the appearance of transfer factor solution.

[0051] 2. Amino acid identification reaction is a positive reaction.

[0052] 3. Ultraviolet spectrophotometric measurement: the sample obtained above has an absorption peak at 261±2nm, and ABS 260 / ABS 280 >1.9.

[0053] 4. The pH value is between 6.0 and 7.5.

[0054] 5. Detection of 20% sulfosalicylic acid: the sample obtained above has no turbidity and precipitation, indicating that the protein reaction is negative, and it does not contain macromolecular protein.

[0055] 6. In the detection of high molecular weight substances, there is no absorption peak before the...

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Abstract

The invention provides a method for extracting transfer factors from cattle spleen, which comprises the following steps of grinding cells by a high-pressure homogenizer, precipitating by a flocculating agent, filtering by a micro-pore filtration membrane, ultra-filtering by a macro-pore filtration membrane, wherein the flocculating agent is added when in shell breaking, so that the protein in solution can be precipitated, and the solution can become transparent, thereby reducing the possibility of blocking a hollow-fiber ultra-filtration column in the ultra-filtration process. The method can greatly shorten the production cycle, save large amount of manpower and material resources and improve the production efficiency of the transfer factors, so as to provide a feasible path for meeting the industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, in particular to a method for extracting transfer factor from animal spleen. The content of the invention mainly relates to a method for extracting transfer factor from bovine spleen. Background technique [0002] Transfer factor (TF) is a substance released by T lymphocytes that can transfer sensitization information. It can specifically transfer the donor's cellular immune information to the recipient, thereby enhancing the immune function of the recipient. Since TF was discovered in the 1950s, scholars at home and abroad have conducted a lot of research on it, and introduced it into veterinary immunity and clinical treatment in 1979. At present, it has become a widely used immune-enhancing agent abroad. [0003] Since transfer factor has no species specificity, clinically there are many sources of transfer factor, such as human transfer factor, porcine transfer factor, bovine transfer facto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P37/04A61K38/02A61K35/26
Inventor 苏建东夏雪林李旭东
Owner TIANJIN RINGPU BIO TECH
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