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Specific antibody against pesticide phosmet

An imophos-specific technology, applied in the field of immunochemistry of pesticide small molecule compounds and pesticide residue analysis, can solve the problems of excessive pesticide residues and affecting the health of agricultural product export trade, etc., achieving high similarity and good specificity and the effect of sensitivity

Inactive Publication Date: 2010-06-23
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

However, the irrational use of pesticides has also led to serious pesticide residues exceeding the standard, affecting people's health and the export trade of agricultural products

Method used

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  • Specific antibody against pesticide phosmet
  • Specific antibody against pesticide phosmet
  • Specific antibody against pesticide phosmet

Examples

Experimental program
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Embodiment Construction

[0036] Hapten Synthesis and Antibody Preparation

[0037] (1) Synthesis of N-phthalimido-6-aminocaproic acid:

[0038] Accurately weigh 0.1250g of aminocaproic acid, 0.0960g of anhydrous Na 2 CO 3 Dissolve in 5.0 mL of distilled water, add 0.255 g of N-phthalimide methyl ester, stir at room temperature until completely dissolved, and terminate the reaction in about 1 hour. Filtration, acid precipitation of the filtrate until the precipitation is complete, extraction with ethyl acetate, anhydrous Na 2 SO 4 After drying and evaporating the solvent, a white solid was obtained with a total yield of 90%. ;

[0039] (2) Synthesis of imophos hapten activated ester:

[0040] Weigh 0.5000g hapten, 0.2400g N-hydroxysuccinimide (NHS) is dissolved in 3ml anhydrous tetrahydrofuran, N 2Under protection, 2 ml of anhydrous tetrahydrofuran solution dissolved with 0.4500 g of DCC was added dropwise, and stirred overnight. The white precipitate was removed by filtration, the organic solv...

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Abstract

The invention relates to a synthetic method of an artificial antigen of phosmet and an antibody preparation process. The invention belongs to the technical field of immunochemistry of small molecule compounds of pesticides and residue analysis and relates to organic synthesis, immunochemistry, biochemistry and the like. The invention solves the problem that the traditional physical and chemical analysis methods are complicated, high in cost and slow in analysis, and provides simple, quick, sensitive and accurate immunoassay. The designed and synthesized hapten N-phthaloyl-6-aminocaproic acid is linked with the carrier protein such as hemocyanin and horseradish peroxidase respectively to synthesize the artificial antigen and an enzyme-labelled antigen. The antibody is prepared through animal immunization of the artificial antigen, blood collection and antiserum extraction and purification. With good stability, high specificity and sensitivity, the antibody can be used for quick immunoassay of the pesticide organophosphorus and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and pesticide residue analysis of small-molecule pesticide compounds (molecular weight less than 1000 Daltons), involving organic synthesis, immunochemistry, biochemistry and physicochemical testing techniques, etc., particularly the hapten N-phthaloyl Design and synthesis of imino-6-aminocaproic acid and preparation of specific antibody. Background technique [0002] Imophos is a non-systemic broad-spectrum acaricide and insecticide. With contact and stomach killing activity, it is suitable for the control of rice, cotton, fruit trees, vegetables and other crop pests and also for the treatment of spider mites. It has a long residual effect and is a substitute for trichlorfon, monocrotophos, parathion and methamidophos Ideal agent for poisonous pesticides. However, the irrational use of pesticides has also led to serious pesticide residues exceeding the standard, affecting people's healt...

Claims

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Application Information

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IPC IPC(8): C07K16/44C07K16/06C07K14/795C07D209/48G01N33/543
Inventor 王硕宋洋王俊平刘冰
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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