Dominant dwarf rice related protein, encoding gene thereof and application
A technology encoding genes and proteins, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve problems such as weakening the inhibitory effect of GA signal transduction
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] Embodiment 1, the isolation of gene
[0046] 1. Phenotype analysis of dwarf mutants
[0047] The applicant of the present invention discovered a dominant dwarf mutant (named Slr1-d4) in the indica rice variety Zhongxian 3037, and crossed the mutant with a normal line (Zhongxian 3037 wild type) to obtain the F1 generation. The plant height of the F1 generation was between the mutant parent and the wild parent, showing good plant height traits and no other bad traits.
[0048] Under normal cultivation conditions, the plant type of the rice mutant Slr1-d4 was significantly different from that of the wild type Zhongxian 3037 ( figure 1 , A represents the wild type Zhongxian 3037 (slr1-d4 / slr1-d4), B represents the heterozygote between Zhongxian 3037 and the mutant (Slr1-d4 / slr1-d4), C represents the homozygous mutant (Slr-d4 / Slr-d4)), mainly manifested in the obvious dwarfing of the mutant, the average final plant height of the mutant (Slr-d4 / Slr-d4) is about 47.6cm, whi...
Embodiment 2
[0074] Embodiment 2, the function of gene
[0075] 1. Functional complementation of dwarfing gene Slr1-d4
[0076] (1) Acquisition of recombinant expression vectors
[0077] The plant expression vector pCAMBIA1300 vector was purchased from Australia, Canberra, Cambia.
[0078] The pCAMBIA1300 vector was double-digested with restriction endonucleases Xmal and Smal. The digested product was ligated with the full-length Slr1-d4 obtained by double digestion with Xmal and Smal, and a complementary plasmid containing the full-length Slr1-d4 gene fragment was obtained. Restriction digestion and sequencing verification showed that the sequence of the gene in the complementary plasmid was constructed and the insertion direction are correct (the sequence of the gene inserted in the complementary plasmid is shown in the 121-6237th nucleotide from the 5' end of sequence 2 in the sequence table), and the positive complementary plasmid is recorded as the recombinant expression vector pCAM...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com