Sheep hemoglobin polypeptide powder and preparation method thereof
A technology of hemoglobin and polypeptide powder, which is applied in the field of food nutrition enhancers, functional foods, and functional food raw materials. It can solve the problems of no sheep hemoglobin polypeptide powder, sheep blood with smell, and difficult food ingredients, etc., and the preparation method is simple. Ease of use, high nutritional value and low cost
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Embodiment 1
[0029] The preparation of embodiment 1 sheep hemoglobin polypeptide powder
[0030] Follow the steps below:
[0031] (1), adding 10 g of trisodium citrate solution with a concentration of 20% to 500 g of sheep blood, stirring evenly, then centrifuging at 5000 g (TGL-16A centrifuge, Changsha Pingfan Instrument Co., Ltd.) for 6 min, separating to obtain two-layer solution , the lower floor is a sheep hemoglobin solution (325g), and the upper floor is a sheep plasma protein solution, and the sheep hemoglobin solution (325g) of the lower floor is collected.
[0032] (2), the goat hemoglobin solution is placed in an airtight container, then evacuated with a vacuum pump, and when the vacuum reaches 0.085 MPa, the temperature is raised to 65° C. and the goat hemoglobin solution is pumped for 3 minutes to obtain a goat hemoglobin solution without mutton.
[0033] (3), dilute the sheep hemoglobin solution obtained in step (2) with water to a concentration of 12%, adjust the pH to 6.5,...
Embodiment 2
[0035] The preparation of embodiment 2 sheep hemoglobin polypeptide powder
[0036] Follow the steps below:
[0037] (1), adding 22 g of 20% trisodium citrate solution to 1000 g of sheep blood, stirring evenly, then centrifuging at 5500 g (TGL-16A centrifuge, Changsha Pingfan Instrument Co., Ltd.) for 6 min, separated to obtain two layers of solution , the lower floor is sheep hemoglobin solution, the upper floor is sheep plasma protein solution, and the sheep hemoglobin solution (655g) of the lower floor is collected.
[0038] (2), the goat hemoglobin solution is placed in a closed container, and then evacuated with a vacuum pump. When the vacuum reaches 0.086 MPa, the temperature is raised to 65° C. and the goat hemoglobin solution is pumped for 4 minutes to obtain the goat hemoglobin solution without mutton.
[0039] (3), dilute the sheep hemoglobin solution obtained in step (2) with water to a concentration of 13%, adjust the pH to 6.5, add 0.9 g of papain, and carry out ...
Embodiment 3
[0041] The preparation of embodiment 3 sheep hemoglobin polypeptide powder
[0042] Follow the steps below:
[0043](1), adding 18 g of 20% trisodium citrate solution to 1000 g of sheep blood, stirring evenly, then centrifuging at 5000 g (TGL-16A centrifuge, Changsha Pingfan Instrument Co., Ltd.) for 8 minutes, separated to obtain two layers solution, the lower floor is a sheep hemoglobin solution, and the upper floor is a sheep plasma protein solution, and the sheep hemoglobin solution (648g) of the lower floor is collected.
[0044] (2), gained goat hemoglobin solution is placed in airtight container, then with vacuum pump pumping, when vacuum degree reaches 0.087 MPa, be warming up to 64 ℃ of pumping for 4 minutes, obtain the goat hemoglobin solution without mutton.
[0045] (3), dilute the sheep hemoglobin solution obtained in step (2) with water to a concentration of 13%, adjust the pH to 6.6, add 1.0 g of papain, and carry out the hydrolysis reaction at 37 ° C and pH 6....
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