Microfluidic chip system integrating cell operation and detection

Abstract

The invention provides a microfluidic chip system integrating cell operation and detection and relates to a device for cell manipulation and detection in early diagnosis of diseases. The microfluidic chip system mainly comprises an alternating current signal power supply, a microchip, an integrated circuit control unit and a computer. The microfluidic chip system is characterized in that: the microchip is formed by bonding polydimethylsiloxane cover glass and a glass substrate, wherein the polydimethylsiloxane cover glass is provided with pipes; the glass substrate is deposited with a cell operation unit electrode and an impedance detection unit electrode; and the integrated circuit control unit is an external circuit control unit, namely, an integrated micro-control circuit board. The microfluidic chip system integrating the cell operation and detection is characterized by high detection sensitivity, good stability, flexible and convenient operation, system micromation, excellent portability, and the like, can be widely applied to the analysis and detection of cells and the early diagnosis of diseases, particularly the acquisition and detection of cancer cells and the early diagnosis of a cancer. The system also can be widely applied to food safety testing, environmental monitoring, medicaments / genes screening, and the like.

Description

technical field [0001] The invention belongs to the technical field of biochemical sample analysis, and in particular relates to a device for manipulating and detecting cells in early disease diagnosis. Background technique [0002] With the advancement of science and technology and the improvement of people's living standards, people's expectations for health are getting higher and higher. They expect early diagnosis of diseases, especially cancer, and require timely and effective treatment. The analysis and detection of biochemical samples, such as proteins, DNA, and cells, is one of the methods to achieve early diagnosis of diseases. It can help doctors to detect the disease as early as possible and take effective treatment measures, which has a very important role and significance in improving people's health. Therefore, the analysis and detection of biochemical samples, especially the manipulation and detection of cells, has attracted more and more attention from scien...

AI Technical Summary

Problems solved by technology

The main disadvantage of this system is that it can only detect the system impedance and cannot realize the manipulation of cells. At the same time, the detection limit is also very high. In the case of few cells, no effective signal can be detected. In the early stage of the disease, the markers in the sample can be analyzed. disease-causing cells are usually few, therefore, the system does not meet the requirements of highly sensitive detection

The main disadvantages of this system are: large-scale instruments such as microscopes and oscilloscopes are needed, and the integration and miniaturization of the DEP analysis system cannot be realized; the circuit control of the whole system is unstable; among them, the gold electrode of the dielectrophoresis chip is also Used for impedance detection, so that cell capture and impedance detection share a pair of interdigitated gold electrodes, which are difficult to coordinate in efficient cell capture and high-sensitivity detection of impedance

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