Method for preparing L (+) potassium hydrogen tartrate

A technology of potassium hydrogen tartrate and potassium tartrate, which is applied in the field of preparation of L potassium hydrogen tartrate, can solve the problems of high cost and many solid wastes, and achieve the effect of saving energy costs and reducing the discharge of waste water and waste

Active Publication Date: 2010-06-16
杭州瑞晶生物科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problem that there are many solid wastes and high costs in the production of existing food-grade potassium hydrogen tartrate, the invention provides a new preparation method of L(+) potassium hydrogen tartrate, which uses direct synthesis ...

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  • Method for preparing L (+) potassium hydrogen tartrate
  • Method for preparing L (+) potassium hydrogen tartrate
  • Method for preparing L (+) potassium hydrogen tartrate

Examples

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Effect test

Embodiment 1

[0020] Example 1: Preparation of immobilized cell particles

[0021] Slant culture: Nocardia tartancans were inoculated in the slant culture medium and cultured at 30° C. for 24 hours. The culture is as follows: yeast powder 3g / L, peptone 5g / L, glucose 10g / L, agar 20g / L, cultured in a culture medium with a natural pH value.

[0022] Seed culture and fermentation: Both the seed and the fermentation medium use liquid medium, and the components of the liquid medium are glucose 10g / L, yeast powder 3g / L, ammonium sulfate 3g / L, anhydrous MgSO 4 0.5g / L, K 2 HPO 4 ·3H 2 O 1g / L, KH 2 PO 4 1g / L; use an inoculation needle to take a ring of bacteria from the slant medium and inoculate it into a 250ml Erlenmeyer flask containing 100ml liquid medium, and cultivate it at 30°C and 180r / min for 24 hours to obtain a first-grade seed solution. The primary seed solution was inoculated in a 300L seed tank containing 200L liquid medium with an inoculation amount of 10%, cultivated for 10h a...

Embodiment 2

[0027] A. Add 49 grams of water into a 500ML glass reactor, add 98 grams of maleic anhydride, 150 grams of 27.5% hydrogen peroxide, and 1 gram of sodium tungstate, slowly add 116 grams of 48% potassium hydroxide dropwise, and control the temperature at 70 Celsius, reacted for 6 hours, the reaction solution was cooled to room temperature, centrifuged, and the precipitate was taken to obtain 150 grams of potassium epoxy succinate, and the mother liquor could be recycled.

[0028] B. In the reaction vessel, add 80 grams of immobilized cell particles prepared by the method in Example 1, then add water to 150 grams of potassium epoxysuccinate obtained in step A to make 1 L of 0.72mol / L solution, and add it to the reaction vessel , keep warm at 35-37°C, shake on a shaker for 18 hours, filter, the filtrate is potassium tartrate solution, add L(+) tartaric acid to adjust the pH to 3.5, centrifuge, take the precipitate, and obtain 260g of L(+) potassium hydrogen tartrate, the purity 99...

Embodiment 3

[0030] The fermented liquid was prepared according to the method of Example 1, and 5 L of the fermented liquid was centrifuged to obtain 50 grams of fermented thallus, which was used for the immobilization of the thalline, and the wet thalline concentration of the fermented liquid was 10 g / L.

[0031] Take 50 grams of fermented cells obtained by centrifugation and mix them with 100 ml of normal saline, stir evenly to obtain a mixed liquid of cells, keep the temperature at 35-37°C for later use;

[0032] Warm up to 100°C with a volume of 250ml of 3% carrageenan solution, stir and mix evenly, cool down to 45°C, then add the bacterial cell mixture, stir well and drop into 2% potassium chloride aqueous solution to form immobilization Particles, the average diameter of the particles is 2mm, solidified for 2 hours, soaked in 0.5% glutaraldehyde for one hour, washed with normal saline, and obtained 400 grams of strengthened immobilized cell particles. Put the immobilized cells into a ...

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Abstract

The invention discloses a method for preparing L (+) potassium hydrogen tartrate. The method is as follows: dissolving epoxy potassium succinate shown as formula II in water, using immobilized cellgranules prepared by fermented thalli obtained by Nocardia strain fermentation as a biocatalyst, carrying out conversion reaction at 35-37 DEG C for 18 hours to form potassium tartrate shown as formula III, dissolving the potassium tartrate in water and reacting with L (+) tartaric acid shown as formula IV until the pH value of reaction liquid ranges from 3.5 to 3.7, thereby preparing the L (+) potassium hydrogen tartrate. The method has the advantages that solid waste is not generated in the process of production, and the added amount of the tartaric acid is half of the added amount of the prior art when equal quality potassium hydrogen tartrate is prepared, thereby saving the energy cost and reducing the discharge of waste water and rubbish by half.

Description

(1) Technical field: [0001] The invention relates to a preparation method of L(+)potassium hydrogen tartrate. (2) Background technology [0002] Potassium hydrogen tartrate chemical name: 2,3-dihydroxy potassium hydrogen succinate, it is a salt of tartaric acid, its appearance is colorless or slightly opaque crystal or white crystalline powder, 1g is dissolved in 165mL of 25℃ water, dissolved in 16mL of boiling water, dissolved in In 8820mL ethanol. Its saturated solution is acidic to litmus. Potassium hydrogen tartrate is divided into "L-potassium hydrogen tartrate" and "DL-potassium hydrogen tartrate". Food Grade Potassium Bitartrate is L-Potassium Bitartrate [0003] Potassium hydrogen tartrate is an internationally recognized safe and non-toxic food additive. Its main function is a leavening agent, and the maximum usage amount in food is different. At present, potassium hydrogen tartrate is a leavening agent commonly used in many countries, which has fully considered...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12N11/10C12R1/365
Inventor 潘晋冀朱洁洁马志高何国田
Owner 杭州瑞晶生物科技有限公司
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