Light-addressable potentiometric sensor for monitoring isolated mouse heart

Abstract

The invention discloses a light-addressable potentiometric sensor for monitoring an isolated mouse heart and use thereof in monitoring surface potential of the isolated mouse heart. The light-addressable potentiometric sensor comprises a reference electrode, a light source and a sensor chip, wherein the light source and the sensor chip are packaged in the cavity; a liquid tank is arranged in an upper cavity of the cavity; the bottom part of the liquid tank is provided with an insulating layer of the sensor chip; the reference electrode is placed in test liquid in the liquid tank; a first groove for holding the light source is arranged in a lower cavity of the cavity; a second groove is arranged the central positions of a working electrode and a substrate of the sensor chip and runs through the working electrode and the substrate; a measurement area of the substrate is arranged over the second groove; the second groove is communicated with the first groove; and the central axes of the liquid tank, the measurement area of the substrate, the second groove and the first groove are coincide. The light-addressable potentiometric sensor can be processed and packaged easily and effectively used for monitoring the isolated mouse heart.

Description

technical field [0001] The invention relates to a biosensor for electrophysiological detection of isolated heart and drug action analysis, in particular to a light-addressing potential sensor for detecting isolated mouse heart. Background technique [0002] In drug monitoring and screening, animal in vivo tests are difficult to screen large quantities of drugs due to complex biological signals, many influencing factors, and high costs. As a new type of sensor, cell sensor has the advantages of high throughput, low cost, and short test period, so it is widely used in the preliminary screening of drugs. However, the drug effect at the cell level is often very different from the actual drug effect in humans and animals. In contrast, drug screening at the tissue and organ level has a higher similarity to the animal body . At present, the microelectrode array (MEA) can effectively measure the pacing characteristics and electrical excitation conduction characteristics of the hea...

AI Technical Summary

Problems solved by technology

However, light-addressable potentiometric sensors for cell potential monitoring must be front-illuminated, which is not possible when such sensors are used for cardiac monitoring because the heart is opaque to light.

Light-addressable potentiometric sensors illuminated by backlight are generally used for cell metabolism monitoring, and are rarely used for the detection of potential signals, and the thickness of the silicon substrate of the sensor is generally less than 100 μm, with poor mechanical strength, easy to break, and difficult to process and package.

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