Extract of carica papaya L. seeds produced in Hainan, preparation method and application thereof
A papaya seed and extract technology, which is applied in the field of comprehensive development and utilization of plant natural products, can solve problems such as unresearched antioxidant activity of papaya extract, and achieve high antioxidant activity, high profit and waste rate and low cost. Effect
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Embodiment 1
[0024] The papaya seed extract provided in this example is prepared by the following method: after soaking the papaya seeds in ethanol with a volume concentration of 90% at room temperature for extraction, the filtrate is rotary evaporated until the ethanol is evaporated to dryness to obtain papaya seeds The crude extract is dissolved in water, extracted with organic solvents petroleum ether, ethyl acetate and n-butanol, and then concentrated to obtain the papaya seed extract.
Embodiment 2
[0026] For the papaya seed extract provided in this example, the papaya seeds are washed with tap water; the washed seeds are dried in the sun until they are 80% dry, and pulverized with a pulverizer; the extract is extracted with ethanol with a volume concentration of 30% at room temperature , the first leaching for 20 days, the second and third leaching in ethanol for 10 days each, after each leaching, filter the leaching solution with a filter cloth in time for recovery, and return the filter residue to the leaching container , the recovered filtrates were combined and stored in a sealed container, and concentrated by rotary evaporation at a constant temperature of 55°C until the ethanol solvent was completely evaporated to dryness, and the crude ethanol extract was accumulated in the rotary evaporator; the total crude extract was used Soluble in water, extract with petroleum ether, ethyl acetate and n-butanol successively, extract continuously for 5 times, and concentrate t...
Embodiment 3
[0028] For the papaya seed extract provided in this example, the papaya seeds are washed with tap water; the washed seeds are dried in the sun until they are 80% dry, and then pulverized with a pulverizer; extracted by ethanol with a volume concentration of 50% at room temperature , the first leaching for 2 weeks, the second and third leaching in ethanol for 1 week each, after each leaching, filter the leaching solution with filter cloth in time for recovery, and return the filter residue to the leaching container In the process, the recovered filtrates were combined and stored in a sealed container; the ethanol extract was concentrated by rotary evaporation at 55°C until the ethanol solvent was completely evaporated to dryness, and the crude ethanol extract was accumulated in the rotary evaporator; the total crude extract The product was dissolved in water, extracted with n-butanol, and extracted continuously for 3 times respectively, and the extract was concentrated by rotary...
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