Silage rich in organic selenium
A technology of silage and organic selenium, which is applied in the preservation method and application of animal feed and animal feed raw materials, can solve the problems of difficult absorption, high price, improper use, etc., to promote healthy growth, improve production performance, and enhance resistance. force effect
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example 1
[0031] (1) Bacteria
[0032] Saccharomyces cerevisiae: purchased from the Collection Center of Microorganisms, Institute of Microbiology, Chinese Academy of Sciences, with the strain code CICC 31011.
[0033]Streptococcus Thermophilus: purchased from the Collection Center of Microorganisms, Institute of Microbiology, Chinese Academy of Sciences, with the strain code AS1.2471.
[0034] (2) Slant activation medium
[0035] Saccharomyces cerevisiae activation medium (YPD): 20g glucose, 5g yeast extract, 10g peptone, 20g agar, 1000ml water, initial pH 6.7, 115°C high pressure steam sterilization for 15min, set aside.
[0036] Streptococcus thermophilus activation medium (MRS): peptone 10g, yeast extract 5g, glucose 20g, beef extract 10g, sodium acetate 5g, diammonium citrate 2g, Tween-80 1ml, MgSO 4 ·7H 2 O 0.02g, MnSO 4 ·4H 2 O 0.05g, K 2 HPO 4 2g, 20g agar, 1000ml water, adjust pH=6.2~6.4; 115°C, high pressure steam sterilization for 15min, set aside.
[0037] (3) No ag...
example 2
[0056] (1) Bacteria
[0057] Saccharomyces cerevisiae: purchased from the Collection Center of Microorganisms, Institute of Microbiology, Chinese Academy of Sciences, with the strain code CICC 31011.
[0058] Streptococcus Thermophilus: purchased from the Collection Center of Microorganisms, Institute of Microbiology, Chinese Academy of Sciences, with the strain code AS1.2471.
[0059] (2) Slant activation medium
[0060] Saccharomyces cerevisiae activation medium (YPD): 20g glucose, 5g yeast extract, 10g peptone, 20g agar, 1000ml water, initial pH 6.7, 115°C high pressure steam sterilization for 15min, set aside.
[0061] Streptococcus thermophilus activation medium (MRS): peptone 10g, yeast extract 5g, glucose 20g, beef extract 10g, sodium acetate 5g, diammonium citrate 2g, Tween-801ml, MgSO 4 ·7H 2 O 0.02g, MnSO 4 ·4H 2 O 0.05g, K 2 HPO 4 2g, 20g agar, 1000ml water, adjust pH=6.2~6.4; 115°C, high pressure steam sterilization for 15min, set aside.
[0062] (3) No ag...
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