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High-efficiency liquid phase chromatographic pre-column derivatization reagent for amino compound and detection method of amino compound

A high-performance liquid chromatography and amino compound technology, which is applied in the field of pre-column derivatization reagents of amino compounds and their detection, can solve the problems of harsh reaction conditions, low detection sensitivity of amino derivatization reagents, interference of chromatographic separation, and the like, To achieve the effect of good repeatability, mild conditions and accurate detection

Inactive Publication Date: 2010-06-09
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its main disadvantages are: some amino derivatization reagents can only react with primary ammonia, but not secondary ammonia, such as OPA; some amino derivatization reagents have harsh reaction conditions and require an anhydrous environment, such as Dansyl-Cl ; Some amino derivatization reagents will form by-products that interfere with chromatographic separation, such as PITC; some amino derivatization reagents have low detection sensitivity, such as DNFB, etc.

Method used

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  • High-efficiency liquid phase chromatographic pre-column derivatization reagent for amino compound and detection method of amino compound
  • High-efficiency liquid phase chromatographic pre-column derivatization reagent for amino compound and detection method of amino compound
  • High-efficiency liquid phase chromatographic pre-column derivatization reagent for amino compound and detection method of amino compound

Examples

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experiment example 1

[0018] Take the detection of seven biogenic amines in beer, including histamine, tryptamine, phenylethylamine, tyramine, putrescine, spermidine and spermine, as an example. Establish the derivatization reaction conditions of biogenic amine standard sample and CNBF as follows: the molar ratio of biogenic amine to CNBF is 1:2; the reaction temperature is 50°C; the reaction time is 80min; The ratio of organic solvent to aqueous solution is 1:5. The HPLC conditions are as follows: C18 chromatographic column is used, the ultraviolet detection wavelength is 254nm, the mobile phase is acetonitrile, acetic acid-sodium acetate buffer (pH6.2), gradient elution is set; the flow rate is 1ml / min; a standard detection curve is established . The beer samples were subjected to pretreatments such as defoaming and concentration, and reacted with CNBF according to the determined reaction conditions, and were detected by HPLC. The contents of seven biogenic amines in beer were calculated through...

experiment example 2

[0021] Take the detection of seven biogenic amines in beer, including histamine, tryptamine, phenylethylamine, tyramine, putrescine, spermidine and spermine, as an example. Establish the derivatization reaction conditions of biogenic amine standard sample and CNBF as follows: the molar ratio of biogenic amine to CNBF is 1:3; the reaction temperature is 60°C; the reaction time is 30min; The ratio of organic solvent to aqueous solution is 1:9. The HPLC conditions are as follows: C18 chromatographic column is used, the ultraviolet detection wavelength is 254nm, the mobile phase is acetonitrile, acetic acid-sodium acetate buffer (pH6.2), gradient elution is set; the flow rate is 1ml / min; a standard detection curve is established . The beer samples were subjected to pretreatments such as defoaming and concentration, and reacted with CNBF according to the determined reaction conditions, and were detected by HPLC. The contents of seven biogenic amines in beer were calculated through...

experiment example 3

[0023] Take the detection of seven biogenic amines in beer, including histamine, tryptamine, phenylethylamine, tyramine, putrescine, spermidine, and spermine, as an example. Establish the derivatization reaction conditions of biogenic amine standard sample and CNBF as follows: the molar ratio of biogenic amine to CNBF is 1:5; the reaction temperature is 70°C; the reaction time is 40min; The ratio of organic solvent to aqueous solution is 1:3. The HPLC conditions are as follows: C18 chromatographic column is used, the ultraviolet detection wavelength is 254nm, the mobile phase is acetonitrile, acetic acid-sodium acetate buffer (pH6.2), gradient elution is set; the flow rate is 1ml / min; a standard detection curve is established . Beer samples were subjected to pretreatments such as defoaming and concentration, and reacted with CNBF according to the determined reaction conditions, and then detected by HPLC. The contents of seven biogenic amines in beer were calculated through th...

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Abstract

The invention discloses a high-efficiency liquid phase chromatographic pre-column derivatization reagent for an amino compound and a derivation condition thereof, which belong to the field of analysis and detection. In the invention, a derivatization reaction is carried out between 3,5-dinitro-4-chloro-trifluorotoluene(CNBF) derivatization reagent and the amino compound undergo derivatization, the detectability and sensitivity of the amino compound in a high-efficiency liquid phase chromatograph are improved, so that the purpose of rapid, accurate and sensitive detection of the amino compound is achieved. In the detection method of the invention, the derivatization is performed under mild conditions and at a high speed and avoids interference peaks, and the detection is accurate, flexible and good in repeatability. The detection method can effectively detect the amino compound and other medicinal or chemical amino compounds in food, feed and environment samples and has an actual promotion and application prospect.

Description

technical field [0001] The invention relates to an amino compound high performance liquid chromatography pre-column derivatization reagent and a detection method thereof, belonging to the field of analytical chemistry. technical background [0002] Amino compounds are amino compounds. Amino compounds are closely related to human daily life and social production development, including amino acids and biogenic amines in food and feed, animal and plant proteins (polypeptides), medical amino products, amino chemical intermediates, amino acids containing Amino agricultural chemicals, etc., are a class of compounds that are vital to humans and the environment, and they are also a class of substances that need to be tested and tracked. With the development of science and technology, the detection methods of this kind of compounds have also made great progress, and the detection method based on high performance liquid chromatography (HPLC) is one of the analysis methods that develop...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N21/33
Inventor 曹永松石天玉钱坤汤涛孙妍李健强刘西莉李卫华侯美军
Owner CHINA AGRI UNIV
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