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Preparation method of nano micellar solution of polymer with simulated cellulosa membrane structure

A nano-micelle solution and imitation cell outer membrane technology, which is applied in the field of biomimetic polymer micelles preparation, can solve the complex and cumbersome synthesis and purification process of block copolymers, affect the biocompatibility of materials and other problems, and achieve structural Compact, easy to popularize and apply, simple preparation process

Inactive Publication Date: 2010-06-09
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the synthesis and purification process of block copolymers is very complicated and cumbersome, and it is difficult to completely remove the copper catalyst contained in the polymer synthesized by ATRP method, which may seriously affect the biocompatibility of the material

Method used

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  • Preparation method of nano micellar solution of polymer with simulated cellulosa membrane structure
  • Preparation method of nano micellar solution of polymer with simulated cellulosa membrane structure
  • Preparation method of nano micellar solution of polymer with simulated cellulosa membrane structure

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Accurately weigh 10 mg of PML70 (MPC:LMA, mol%:mol%, 7:3), and dilute to 10 ml with twice distilled water. Sonicate for 1 min, shake to dissolve the polymer completely, then shake at 60r / min for 6h. After being stirred at 50° C. for 90 minutes, a nano-micelle solution with a compact structure and a narrow particle size distribution is obtained, and the diameter of the micelles is 200-400 nanometers as determined by a scanning electron microscope.

[0038] The comparative example shows that the nano micelles without stirring and heat treatment at 50°C have a loose structure and a wide particle size distribution, with particle diameters ranging from 50 to 800 nanometers.

Embodiment 2

[0040] Weigh 10 mg of PMS30 (MPC: SMA, mol%: mol%, 3: 7), add 100 microliters of THF dropwise thereto to dissolve the polymer, add double-distilled water dropwise to it under stirring to settle to 10 ml, The THF in the solution was volatilized by stirring openly. After 2 hours, stir at 80°C for 10 minutes, cool to room temperature and centrifuge at 7980r / min for 10 minutes to remove the precipitate to obtain an aqueous solution of PMS30 polymer micelles with a concentration of 0.53mg / ml. The diameter of micellar particles was determined by scanning electron microscope to be 100-200 nanometers.

[0041] The comparative examples show that the nano-micelles without heat treatment at 80°C have a loose structure and a wide particle size distribution, with particle diameters ranging from 30 to 500 nanometers.

Embodiment 3

[0043] Taking the polymer PML30 (MPC:LMA=3:7) as an example, the process of measuring the critical micelle concentration and micelle structure of MPC polymer with better water solubility by pyrene fluorescent probe method is described.

[0044] (1) With anhydrous acetone as solvent, the concentration of pyrene prepared is 1.37×10 -4 mol / L acetone solution, ultrasonic treatment for 10min;

[0045] (2) Accurately weigh 10mg of PML30 in a 10ml volumetric flask, dissolve it with double distilled water to a constant volume, treat it with ultrasonic wave for 3min, and then shake it with an oscillator at 60r / min for 12h. Promptly obtain the PML30 polymer micelle solution that concentration is 1.0mg / ml;

[0046] (3) Use a micro-sampler to add 10 microliters of 1.37×10 to several 10ml brown volumetric flasks respectively. -4 mol / l pyrene solution in acetone, placed in a fume hood in the dark and exposed for 10 minutes to allow the acetone to volatilize naturally;

[0047] (4) Concen...

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Abstract

The invention discloses a preparation method of nano micellar solution of polymer with simulated cellulosa membrane structure. The preparation method comprises the following steps: (1) preparing polymer with simulated cell membrane structure with mass ratios of MPC:LMA being 3:7-7:3 and MPC:SMA being 3:7-7:3(2) preparing micellar solution of the polymer; (3) agitating the prepared micellar solution of the polymer at 50-90 DEG C for heat treatment for 5-90min; and (4) cooling and filtering to obtain the nano micellar solution of polymer with simulated cellulosa membrane structure. The invention prepares micellar solution of polymer by simple and convenient direct dissolving method and solvent volatilizing method, and obtains nano micellar solution with compact structure and narrower granulometric distribution after treatment of agitating for 5-90min at 50-90 DEG C.

Description

technical field [0001] The invention relates to a preparation method of a biomimetic polymer micelle, in particular to a method for preparing a micelle with an imitation cell outer layer membrane structure by using a random copolymer containing a phosphorylcholine hydrophilic group and an alkyl hydrophobic chain. Background technique [0002] As a drug carrier, polymer nanomicelle encapsulates the drug, which can not only solubilize the poorly water-soluble drug, but also deliver the drug to the target site through passive targeting, thereby reducing toxic side effects and significantly improving the bioavailability of the drug. The polymer nanomicelle can prevent the drug from being hydrolyzed in an acidic environment, greatly reducing the chance of contact between the drug and pepsin. For example, polymer nanomicelles are used to encapsulate peptides and protein drugs that are easily destroyed by intestinal enzymolysis, and after oral administration, they can pass through ...

Claims

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Application Information

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IPC IPC(8): C08J3/03C08J3/09C08L33/14C08L33/10A61K9/00A61K47/32
Inventor 马佳妮党媛宫永宽
Owner NORTHWEST UNIV
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