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Paenibacillus polymyxa and application thereof

A polymyxa and bacillus technology, applied in the application, bacteria, bacterial peptides and other directions, can solve the problems of damage to the host vascular bundle transport tissue, easy loss of resistance, low resistance, etc. Effect

Inactive Publication Date: 2012-05-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although different plants show some differences in symptoms and damage degrees under different environmental conditions, the common feature is that bacteria invade from the roots of the plants, and damage the host vascular bundle transport tissue through proliferation and a series of biochemical activities, resulting in dehydration, wilting and death of the plants
So far, it is rare to control bacterial wilt by using crop varieties resistant to bacterial wilt, because the varieties that have emerged are not only low in resistance but also easy to lose resistance. Other agricultural control methods are limited by geographical conditions. Difficult to promote on a large scale
In terms of chemical control, although there are agricultural streptomycin, copper preparations and other drugs, none of them are pesticides for the control of bacterial wilt, and the control effect is poor, unstable, and pathogenic bacteria are prone to develop drug resistance. Effective agent for plant bacterial wilt

Method used

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  • Paenibacillus polymyxa and application thereof
  • Paenibacillus polymyxa and application thereof
  • Paenibacillus polymyxa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0030] Example 2 strain identification

[0031] The main morphological and biological characteristics of the strains isolated above are as follows: the cells are rod-shaped, Gram-positive, and move with perinatal flagella. There are oval spores in enlarged cysts, and there is no soluble pigment on nutrient agar. Facultative anaerobic. The colony on the culture medium is thin, expanding in a star shape, with a large amount of bacteria and sticky, and the surface is dull. Growth will adhere to the medium. The optimum temperature for growth is 28-30°C, and the growth temperature range is 15-50°C. The optimum pH for growth is 7.0, and the growth pH range is 5.6-8.5.

[0032] The strain was identified as Paenibacillus polymyxa by FAME fatty acid analysis and named as Paenibacillus polymyxa ZJU0901.

Embodiment 3

[0033] Antagonistic activity of embodiment 3 bacterial strain ZJU0901 to Ralstonia solanacearum

[0034] In this example, the 8 R. solanacearum strains in Example 1 were still used as indicator strains. Add 1ml of the bacterial suspension of the indicated strain to 15ml of NA medium that has been sterilized and melted and cooled to about 45°C, shake well and pour the medium into a sterilized petri dish. Use a sterilized toothpick to pick a single colony of Paenibacillus polymyxa ZJU0901, and spot it on the medium with indicator bacteria. The culture condition is 30°C for 48 hours, and its antagonistic ability is determined according to the diameter of the inhibition zone. Antagonism results are as follows (Table 2):

[0035] Table 2 Antagonistic activity of Paenibacillus polymyxa ZJU0901 to Ralstonia solanacearum strains from different hosts

[0036]

[0037] The above results show that: Paenibacillus polymyxa ZJU0901 has obvious antagonistic activity to other indicator p...

Embodiment 4

[0038] Antibacterial activity assay of embodiment 4 Paenibacillus polymyxa ZJU0901 different treatments

[0039] The antibacterial activity of Paenibacillus polymyxa ZJU0901 bacterial suspension, sterile filtrate, proteinase K-treated filtrate and high-temperature heat-treated filtrate were determined by the Oxford cup method, and each treatment was set in 3 repetitions, and the specific methods for each treatment as follows:

[0040] (1) Bacterial suspension preparation: Pick a single colony of Paenibacillus polymyxa ZJU0901, inoculate it in NB liquid medium, and culture it with shaking at 170 r / min at 30°C for 24 hours;

[0041](2) Preparation of sterile filtrate: the bacterial suspension was centrifuged at 10,000 r / min for 20 min, the supernatant was taken, and sterilized with a 0.22 μm microporous membrane to obtain a crude extract of antibacterial substances;

[0042] (3) Proteinase K treatment: take 1ml of the sterile filtrate, divide it into 1.5ml centrifuge tubes, add...

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Abstract

The invention discloses a Paenibacillus polymyxa strain for preventing and controlling plant bacterial wilt, which is named Paenibacillus polymyxa ZJU0901 through identification and is conserved in China Center for Type Culture Collection (CCTCC) positioned in Wuhan University, Luojia Hill, Wuhan. The conservation date of the strain is July 19th, 2009, and the conservation number of the strain is CCTCC NO:M209157. The strain can produce inhibiting effect on the growth of different biochemical types of strain of ralstonia solanacearum under in vitro conditions and can effectively prevent and control plant bacterial wilt.

Description

technical field [0001] The invention relates to a Paenibacillus, in particular to a Paenibacillus macerans and application thereof. Background technique [0002] Bacterial wilt of crops is a systemic and devastating soil-borne disease caused by Ralstonia solanacearum (commonly known as Ralstonia solanacearum), commonly known as "plant blast". The stems and leaves of the affected crops wilt and sag until the whole plant dies. The incidence rate of the general field is 10%-15%, and the incidence rate of the severely diseased field is as high as 80%-99%, resulting in a serious decline in crop yield or even failure. The pathogen has a wide range of hosts and can infect hundreds of plants in 44 families, including tomatoes, eggplants, tobacco, peanuts, potatoes, bananas, etc. [0003] Although there are some differences in the symptoms and damage levels of different plants under different environmental conditions, the common feature is that bacteria invade from the roots of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C07K14/195A01N63/00A01P1/00C12R1/01
Inventor 李斌苏婷余山红刘宝平谢关林
Owner ZHEJIANG UNIV
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