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Detection of environmental pollutants by using Photobacterium leiognathi YL bacterial strain

A technology for environmental pollutants and strains, applied in the field of microorganisms, can solve problems such as detection of environmental pollutants without luminescent bacteria

Inactive Publication Date: 2010-05-26
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except for Luminescent bacteria, there is no report on the use of other species of luminescent bacteria to detect environmental pollutants.

Method used

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  • Detection of environmental pollutants by using Photobacterium leiognathi YL bacterial strain
  • Detection of environmental pollutants by using Photobacterium leiognathi YL bacterial strain
  • Detection of environmental pollutants by using Photobacterium leiognathi YL bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Common seafood caught from the coastal waters of Qingdao, including squid, prawns, yellow croaker, blackhead, monkfish, etc. Divide various aquatic products into small pieces and place them on solid medium plates, culture them in a water-proof constant temperature incubator at 25°C for 10 hours, and observe the luminescence in a dark room. Luminescent colonies were marked and streaked on plates. After several times of separation and purification, the luminescent bacteria YL was obtained.

Embodiment 2

[0017] Take a single colony of luminescent bacteria YL and inoculate it in 100ml liquid medium (medium composition: peptone 5g, yeast extract 1g, FePO 4 0.1g, NaCl 20g, glycerin 3ml, distilled water 1000ml), cultured on a shaking table at 27°C for 8hrs to make the bacteria reach the terminal logarithmic growth phase.

[0018] Take 5ml of heavy metal zinc solutions of different concentrations and add them to 5ml of bacterial solution, and incubate and react on a shaking table at 27°C for 20min. After the reaction is completed, measure the luminescence value in a weak luminescence measuring instrument for 50 s, and take the average value of the results. It can be seen from the measurement results that the inhibition rate and Zn 2+ Concentration is proportional to ( Figure 4 ), so when measuring this kind of pollutant in food or environment, the content of the pollutant can be inferred according to the inhibition rate.

Embodiment 3

[0020] Take a single colony of luminescent bacteria YL and inoculate it in 100ml liquid medium (medium composition: peptone 5g, yeast extract 1g, FePO 4 0.1g, NaCl 20g, glycerin 3ml, distilled water 1000ml), cultured on a shaking table at 27°C for 8hrs to make the bacteria reach the terminal logarithmic growth phase.

[0021] Take 5ml of heavy metal copper solutions of different concentrations and add them to 5ml of bacterial solution, and incubate and react on a shaking table at 27°C for 20min. After the reaction is completed, measure the luminescence value in a weak luminescence measuring instrument for 50 s, and take the average value of the results. From the measurement results, it can be seen that the inhibition rate and Cu 2+ The concentration is proportional to ( Figure 5 ), so when measuring this kind of pollutant in food or environment, the content of the pollutant can be inferred according to the inhibition rate.

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Abstract

The invention aims at providing a Photobacterium leiognathi YL bacterial strain deriving from marine environment and quantitative detection on environmental pollutants by using the bacterial strain. In the invention, the luminous bacterial YL deriving from the marine environment is obtained by separating and purifying, is Gram-negative bacilli, and is rhabditiform. The luminous bacterial YL is identified to be Photobacterium leiognathi according to a phylogenetic tree established by a 16SrDNA sequencing result and a physiology and biochemistry result. The Photobacterium leiognathi YL is preserved in China Center for Type Culture Collection (CCTCC in short) with a collection number of M 206139. The 16SrDNA gene sequence of the luminous bacterial is submitted to GenBank nucleotide sequence database with the accession number of EF017227. The bacterial strain has the characteristics of rapid growth, stable luminous property, low requirements for nutrients, and sensibility to the environmental pollutants, and the like; the luminous intensity thereof is confirmed to form a maximum peak at 474 nm by fluorescence scanning; and the luminous intensity inhibition ratio thereof is in direct ratio to the concentration of multiple tested environmental pollutants, thereby the quantitative detection of the tested environmental pollutants can be realized. The invention has the characteristics of low cost, simple and convenient operation, high sensitivity and the like on detecting the environmental pollutants, , thereby satisfying the requirements on rapid detection of the pollutants.

Description

technical field [0001] The invention relates to a microorganism, in particular to a marine luminescent bacterium Photobacterium leiognathi YL strain which can be used for the detection of environmental pollutants. Background technique [0002] According to the inventor's information and literature search, there are only three known genera of marine luminescent bacteria, which are Vibrio, Photobacterium, and Shewanella. On land, there are different short bacillus (Xenorhabdus). In an aerobic natural environment, luminescent bacteria can produce luciferase (LE), catalyze flavin mononucleotide (FMNH 2 ) and long-chain aliphatic aldehydes undergo oxidation reactions to emit blue-green fluorescence. [0003] At present, Photobacterium phosphoreum is widely used in our country. There are some reports on the use of Photobacterium phosphoreum to detect environmental pollutants. Except for Luminescent bacteria, there is no report on using other species of luminescent bacteria to d...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/02C12R1/01
Inventor 王静雪林洪朱兰兰王亚群
Owner OCEAN UNIV OF CHINA
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