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Method for performing enzyme hydrolysis on wood fiber raw material

A lignocellulosic raw material and lignocellulosic technology, applied in the field of enzymatic hydrolysis, can solve problems such as impossible to meet, and achieve the effects of shortening time, reducing inhibition, and improving hydrolysis yield and

Active Publication Date: 2010-05-12
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the constraints of food and arable land shortages, it is impossible to produce ethanol using food or sugar as raw materials to meet the demand for fuel ethanol in the rapidly developing automobile industry. Therefore, it is necessary to study how to use renewable lignocellulosic raw materials for large-scale production Fuel ethanol has become an important topic at present

Method used

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  • Method for performing enzyme hydrolysis on wood fiber raw material
  • Method for performing enzyme hydrolysis on wood fiber raw material
  • Method for performing enzyme hydrolysis on wood fiber raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Pretreatment of Corn Stalks by Cellulase Three-stage Hydrolysis and Steam Explosion

[0030] 1. Use corn stalks pretreated by steam explosion and water washing as lignocellulosic raw materials, the solid-liquid weight ratio is 1:3-20, the amount of cellulase is 5-25 FPIU / g cellulose, the pH value is 4-6, and the temperature is 45 Under the condition of ~50°C, low-speed agitation hydrolysis for 6 hours, followed by solid-liquid separation.

[0031] 2. Centrifuge the hydrolyzate at 4000 rpm for 10 min, collect the supernatant, measure the content of cellobiose and glucose in the sugar solution, and calculate the yield of cellulase hydrolysis. The enzymolysis residue obtained by centrifugation was washed twice with distilled water, centrifuged at 10,000 rpm for 5 min, and the supernatant and enzymolysis residue were collected respectively.

[0032] Determination of sugar concentration: using high performance liquid chromatography (HPLC) method. Chromatography ...

Embodiment 2

[0048] Example 2: Homogeneous hydrolysis of cellobiose sugar solution by β-glucosidase and recovery and reuse of β-glucosidase by ultrafiltration

[0049] 1. Combine the sugar liquid obtained by segmental hydrolysis in Example 1, evaporate and concentrate under reduced pressure until the concentration of cellobiose is 30g / L.

[0050] 2. Add β-glucosidase, buffer and distilled water to the above-mentioned concentrated sugar solution, the addition amount of β-glucosidase is 1 and 2IU / ml respectively, control the pH value of the hydrolysis system to 4-6, and the temperature of 45-50°C Stir at low speed and hydrolyze for 1-6 hours.

[0051] 3. After the hydrolysis, measure the content of cellobiose and glucose in the hydrolyzate, and calculate the hydrolysis yield of cellobiose hydrolyzed by β-glucosidase. The β-glucosidase in the hydrolyzate was recovered by an ultrafiltration membrane with a molecular weight cut-off of 30kDa, the enzyme activity of the β-glucosidase was measure...

Embodiment 3

[0055] Example 3: Cellulase two-stage hydrolysis steam explosion pretreatment of corn stalks

[0056] Referring to the method of Examples 1 and 2, the process is simplified by adopting (12+12)h two-stage hydrolysis method, and the 24h cellulose hydrolysis yield is about 59.12%. See the following table for the stage-by-stage yield of two-stage or three-stage enzymatic hydrolysis process:

[0057] Segmented enzymatic hydrolysis results of steam-exploded corn stalks

[0058]

[0059] Regardless of the two-stage method or the three-stage method of enzymatic hydrolysis process, the specific subsection time of solid-liquid separation can be changed within a certain range considering the convenience of production scheduling.

[0060] Although the efficiency and yield of enzymatic hydrolysis can be improved to a certain extent by adopting more staged solid-liquid separation treatment process, it will increase the cost of treatment, so it is not considered.

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Abstract

The invention discloses a method for performing enzyme hydrolysis on a wood fiber raw material, which uses the pretreated wood fiber as a raw material and is characterized in that the method adopts processes of stage enzymolysis and ultra-filtration to recycle cellulase and beta-glucosidase. Due to timely removal of cellobiose and glucose in the reaction process, the method greatly relieves the inhibiting effect of a reaction product on the cellulase, improves the hydrolysis yield of cellulose and the efficiency of converting the cellobiose into the glucose, and can obviously shorten the time of enzymolysis reaction.

Description

1. Technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis in the biodegradation and transformation of lignocellulosic raw materials to produce fuel ethanol, chemical platform compounds and other bio-based chemicals, and particularly relates to a method of hydrolyzing lignocellulose in stages with cellulase and recovering β- glucosidase method. 2. Background technology [0002] It is predicted that the available petroleum resources on the earth will be exhausted in the next few decades. In the long run, the shortage of liquid fuels will be a major problem that plagues the development of human society. Scientists from various countries have been actively looking for new energy sources that can replace or partially replace petroleum. Bioethanol is recognized as the best substitute of gasoline due to its outstanding advantages such as renewability, environmental protection and no greenhouse effect during use, and has been extensively studied...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCY02E50/16Y02E50/10
Inventor 余世袁杨静勇强
Owner NANJING FORESTRY UNIV
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