Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DREBs transcription factor GsDREBa of wild soybean and base sequence thereof

A technology of wild soybean, transcription factor, applied in the direction of DNA/RNA fragment, recombinant DNA technology, plant peptide, etc., can solve the problem of restricting the promotion and use of abiotic stress genetic engineering

Inactive Publication Date: 2010-05-12
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DREB (Dehydration ResponsiveElement Binding protein) transcription factor is a relatively hot protein factor in non-stress molecular biology research, but there are relatively few DREB transcription factors that are actually available for use, which limits the promotion and use of abiotic stress genetic engineering

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DREBs transcription factor GsDREBa of wild soybean and base sequence thereof
  • DREBs transcription factor GsDREBa of wild soybean and base sequence thereof
  • DREBs transcription factor GsDREBa of wild soybean and base sequence thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0013] Embodiment 1: In this embodiment, the DREB transcription factor GsDREBa of wild soybean has abiotic stress resistance and has one of the following amino acid sequences:

[0014] ①As shown in SEQ ID NO: 1;

[0015] ② Substitution, deletion or superposition of one or several amino acids in the amino acid sequence defined in ①.

[0016] Embodiment ② includes an amino acid sequence having at least 80% homology, especially at least 95% homology, with the amino acid sequence of SEQ ID NO: 1.

[0017] 本实施方式野生大豆的DREB类转录因子GsDREBa都含有保守的AP2 / EREBP结构域序列(ThrGlnAsnSerGlnCysAsnTyrArgGlyValArgGlnArgThrArgGlyLysTrpValGlyGluIleArgGluProAsnArgGlySerArgLeuTrpLeuGlyThrPheSerSerGlnProGluAlaAlaLeuAlaTyrAspGluAlaAlaArgAlaMetTyrGlyProCysAlaIleLeuAsnPhe)。

specific Embodiment approach 2

[0018] Embodiment 2: The DREB transcription factor GsDREBa of wild soybean encoded by the nucleotide sequence of the wild soybean DREB transcription factor GsDREBa (as shown in SEQ ID NO: 1) in this embodiment has abiotic stress resistance.

[0019] The base sequence of this embodiment can be used to construct the expression vector of the GsDREBa gene. When GsDREBa is used to construct the plant expression vector, any enhanced promoter or inducible promoter can be added before the transcription start nucleotide. In order to facilitate the identification and screening of transgenic cells or plants, the used vectors can be processed, including adding plant screening marker genes and reporter genes. Selectable markers that can be used include enzymes that are resistant to antibiotics, including gentamicin, kanamycin, hygromycin, and the like. Enzymes that produce compounds that are recognized by a color change (eg, GUS) or luminescence (eg, GFP), or resistant chemical agents (eg,...

specific Embodiment approach 3

[0021] Specific embodiment three: this embodiment obtains the DREB type transcription factor GsDREBa of wild soybean according to the following steps:

[0022] 1. Cloning and analysis of the conserved region of DREBP / AP2:

[0023] Wild soybean seedlings were treated in 0.2mol / L NaCl for 1 hour, and the leaves were quickly frozen in liquid nitrogen for RNA extraction; total RNA was extracted from leaves using Trizol Reagent, and the operation was referred to the instructions of Invitrogen’s Trizol reagent; multiple sequence alignment Amino acid sequences of Arabidopsis DREB2A, DREB2B (AB007790, AB007791), tomato DREB2 (AF500012), maize DREB2 (AF450481), a simplified set of β-sheet and α-helical regions of the relatively conserved DREBP / AP2 domain The degenerate primer sequence is as follows: GsAP2(+): 5'-GGH(A / T / C)AAATGGGTD(T / A / G)K(G / T)S(C / G)H(A / T / C)GA-3' and GsAP2(-):5'-GGR(G / A)AAR(G / A)TTR(G / A)AGD(A / T / G)M(A / C)K( G / T) AGC-3'. The RNA extracted from wild soybean leaves was r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a DREB transcription factor GsDREBa of a wild soybean and a base sequence thereof, relating to the DREB transcription factor GsDREBa and the base sequence thereof. The invention solves the problem that currently practical utilized DREB transcription factor is less. The DREB transcription factor GsDREBa of the wild soybean has abiological stress resistance and comprises one of the following amino acid sequences: 1. the sequence is expressed in SEQ ID NO: 1; and 2. one or more of amino acids are substituted and lost or overlapped in the amino acid sequence defined by the step 1. The DREB transcription factor GsDREBa of the wild soybean coded by the base sequence of the DREB transcription factor GsDREBa of the wild soybean has the abiological stress resistance. The invention can be used for plant abiological stress gene engineering.

Description

technical field [0001] The invention relates to a DREB type transcription factor GsDREBa and its base sequence. Background technique [0002] Adverse conditions such as low temperature, drought and salinity seriously affect the yield and quality of crops, and are major problems restricting agricultural production in Northeast China and even the whole country. Improving the ability of crops to tolerate low temperature, drought and salinity is one of the main goals of agricultural production. Under abiotic stress conditions, higher plant cells will actively mobilize the defense system in the body, from perception to signal transduction, and finally transmit the signal to the transcription factor in the nucleus. The transcription factor acts on the functional gene to start the expression of the stress response gene, resulting in Synthesis of new proteins, transformation of metabolism and accumulation of anti-reverse osmotic substances, etc., ultimately achieve the purpose of i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/415C12N15/11
Inventor 朱延明才华柏锡纪巍李勇季佐军
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com