Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

5-alpha reductase inhibitor-nettle diffractive cyclolignan anthocyanin C, preparation method and application thereof

A technology of reductase inhibitor and schizolignan, which is applied in the field of extraction and preparation of biologically active components of traditional Chinese medicine, can solve the problems of weak effect, and achieve the effect of low cost and simple and easy operation

Inactive Publication Date: 2010-05-12
DALIAN UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported abroad that the lignans in nettle have certain anti-BPH activity, but the effect is weak, and the split-ring nettle lignans have a special structure, and their effect is equivalent to that of the positive drug finasteride

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 5-alpha reductase inhibitor-nettle diffractive cyclolignan anthocyanin C, preparation method and application thereof
  • 5-alpha reductase inhibitor-nettle diffractive cyclolignan anthocyanin C, preparation method and application thereof
  • 5-alpha reductase inhibitor-nettle diffractive cyclolignan anthocyanin C, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The medicinal materials of split-leaf nettle are collected from Aba Prefecture, Sichuan Province. The method steps of preparing nettle split cyclolignan C from Urtica split leaf are as follows:

[0044] (1) After crushing the dried underground part of Urtica schizophyllum, it is reflux extracted with industrial ethanol with a mass concentration of 95%, the ratio of solid to liquid (kg / l)=1:8, the extraction temperature is 90°C, the extraction time is 3h, and the extraction The number of times is 3 times, the extracts are combined, filtered and concentrated under reduced pressure until there is no alcohol smell;

[0045] (2) Suspend 200 g of the ethanol extract with 2 L of hot water, and extract with 2 L of petroleum ether, ethyl acetate, and water-saturated n-butanol successively, each extracting 3 times;

[0046] (3) 30 g of the raffinate aqueous layer extract was separated by D101 macroporous resin (1 kg), eluted with an ethanol-water gradient (water → 30% ethanol → ...

Embodiment 2

[0050] The 5-alpha reductase inhibitory effect of Urtica schizolignanside C prepared in Example 1 was tested.

[0051]5-α reductase inhibition experimental steps are as follows:

[0052] (1) Preparation of 5-alpha reductase:

[0053] Three male SD rats, weighing 200±10g, were provided by the Experimental Animal Center of Dalian Medical University. They were fasted and killed overnight, and the ventral prostate tissue was quickly taken out and cut into pieces on an ice table. mol L -1 , calcium chloride 1.91mmol·L -1 ) was homogenized in a glass homogenizer, centrifuged at 10,000 rpm at low temperature and high speed for 20 min at 4°C, and the supernatant was centrifuged at 16,000 rpm for 30 min at low temperature and high speed to obtain the 5α-reductase extract. The protein content was determined by the Lowry method, and the total protein content in the enzyme extract was used to represent the content of the 5α-reductase extract. The extract was placed in a small centrifug...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a 5-alpha reductase inhibitor-nettle diffractive cyclolignan anthocyanin C, a preparation method and application thereof. The preparation method comprises the following steps: taking the underground part of urtica triangularis as raw material; ethanol extracting; mix suspending ethanol extractive with hot water; extracting petroleum ether, ethyl acetate and water saturated n-butyl alcohol; separating raffinate layer of extractive with macroporous resin and eluting; and separating and purifying by means of silica gel column chromatography. The nettle diffractive cyclolignan anthocyanin C can obviously inhibit the generation of DHT, wherein the effect is as the same as positive finasteride, and has potential use for the prevention and the treatment of prostate disease. The invention takes the diffractive cyclolignan anthocyanin as the raw material which is the root of a pure natural plant, and is safely and easily obtained, has simple and convenient operation, does not introduce toxic substances; has low cost, has strong effect although from the plant, and has very important meanings for developing an anti-benign prostatic hyperplasia medicine.

Description

technical field [0001] The invention belongs to a method for extracting and preparing biologically active components of traditional Chinese medicines, in particular to preparing effective plant components from plant nettle schizophyllum by means of dissolving, extracting, extracting, centrifuging, adsorbing and eluting, and has potential effects on prostate and other diseases. Therapeutic effect. Background technique [0002] Kraus and Wu et al. [1-2] Thirteen lignans were identified from Stinging nettle: (+)-Neooliveresin, (-)-Seconoisolaricin, Isolaricin, Pinesinol, 3,4-Di Vanillyl Tetrahydrofuran, Neo-Olivoresin-4-O-β-D-Glucoside, 9-Acetyl-Neo-Olivoresin, 9-Acetyl-Neo-Olivoresin-4-O-β-D -glucoside, 9,9'-diacetyl-neoleoresin, 9,9'-diacetyl-neoleoresin-4-O-β-D-glucoside, (-)-ring Isolaricin-9-O-β-D-glucoside, (-)-Secoisolaricin-4-O-β-D-glucoside, Neooliveresin-9-O-β-D - Glucosides. Its structural types mainly include split-ring larchoresin, larchoresin and its glycosid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H15/26C07H1/08A61K31/7048A61P13/08
Inventor 姜妮冯宝民
Owner DALIAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com