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Method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori

A technology of Aspergillus awamori and cellulase, which is applied in the field of preparation of cellulase and polypeptide protein feed produced by fermentation of Aspergillus awamori strains, and can solve problems such as anemia, death, and limitation of peanut meal feeding amount

Inactive Publication Date: 2010-05-05
武汉博普奥多肽生产技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor composition of amino acids in peanut meal, the aflatoxin produced by Aspergillus flavus, etc., the feeding amount of peanut meal is also limited to a certain extent; rapeseed meal contains toxic substances such as glucosinolate and erucic acid, which makes rapeseed meal The application of meal has been greatly restricted, most of which are used as fertilizer, and less than 30% are used for feed; cottonseed meal contains gossypol and other toxic substances, which can cause animal growth to be hindered, production capacity to decrease, anemia, dyspnea, reproduction, etc. Decreased ability or even infertility, can cause death in severe cases

Method used

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  • Method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori
  • Method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori
  • Method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Isolation, purification and screening of strains

[0057] A. Sampling and enrichment: The first is sampling. From the collection to 70 samples, the samples come from soil samples, dead branches and rotten leaves (cypress, pine, palm, etc.), PKC samples, and rotten plant stems and leaves (bamboo). Leaves, grass, etc.) etc. After sampling, use a sterile bag for packaging. Store in the refrigerator at 0~4℃. The second is enrichment. PKC pretreated by extrusion and gas explosion is used as the only carbon source, and the enrichment medium is configured with inorganic salt. Each test tube is filled with 4ml and sterilized. After sterility test, 1g sample was inserted, placed at 20~45℃, 220r / min shaking enrichment culture for 5~7 days. The inoculation of each medium was repeated once for a total of 280 culture tubes.

[0058] Isolation and purification of strain B: a. After separation, after enrichment and culture, each test tube is lined on three plates with 5 medi...

Embodiment 2

[0060] Example 2: Classification and identification of strains

[0061] In the process of separation and screening, we noticed the growth characteristics, morphological structure and color production of strain 1042-2. In addition to the above research, the use of culture medium, biomass and enzyme production kinetics research, electron microscopy and scanning electron microscopy observation, and 18SrDNA molecular biology identification have been added. The results are as follows.

[0062] 1042-2 On the PDA plate medium, the hyphae are initially white and fluffy, and grow sparsely radiating to the surroundings; they gradually become yellowish-gray, and the colonies are abnormally thick and dense, such as Pic 4-1 ; Subsequently, the colony turns brown to reddish brown or dark brown; the conidia are brown reddish brown or dark brown, and there are a lot of them. The conidial socket is head-shaped, with an opening and thorns outside. The diameter of the conidia is 3.5μm. Such as Figu...

Embodiment 3

[0064] Example 3: Enzyme production test by solid fermentation

[0065] Three strains of 1042-2, 2221-P and 13005 obtained by three-stage screening were subjected to a shallow plate solid fermentation test for enzyme production. The formula of the shallow plate solid medium is as described in the content of the invention. After testing, the CMC enzyme activity and FPA enzyme activity produced by the two strains 1042-2 and 2221-P were higher than those produced by 13005. The applicant selected the two strains 1042-2 and 2221-P for further shallow plate fermentation experiments. The test results averaged that the CMC enzyme activity of 1042-2 was 20331.3 U / g, the FPA enzyme activity was 1116.6 U / g; the CMC enzyme activity of 2221-P was 23431.0 U / g, and the FPA enzyme activity was 966.4 U / g; 13005 The CMC enzyme activity was 6299.4 U / g, and the FPA enzyme activity was 679.9 U / g. After comprehensive evaluation and analysis, the newly screened strains of 1042-2 and 2221-P were continu...

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Abstract

The invention discloses a method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori 1042-2 which belongs to the Aspergillus and has the collection registration number of M209142 in the China Center for Type Culture Collection (CCTCC). The method for producing the cellulose and the polypeptide protein feed comprises the following steps: A, selecting the Aspergillus awamori 1042-2; B, authenticating the Aspergillus awamori 1042-2, C, culturing the Aspergillus awamori 1042-2 liquid; D, fermenting the solid material; and F, postprocessing. The solid culture which is obtained by inoculating the Aspergillus awamori 1042-2 onto the solid culture medium and fermenting the Aspergillus awamori 1042-2 and the solid culture medium is baked to dry, ground, sieved and subjected to the detection of the enzyme activity and the polypeptide protein content. The method for producing the cellulose and the polypeptide protein feed is feasible and easy, the produced powdered cellulose has the CMC enzyme activity of 31397.1-41826.7 U / g and the FPA enzyme activity of 1779.3-2212.1 U / g, has good stability and can be produced on a large scale, and the produced polypeptide protein feed contains more than 35 percent of protein. The Aspergillus awamori 1042-2 can be used for fermenting the palm kernel dregs, other dregs and the vegetable fiber and producing the biological feed, the biological fertilizer, the biological fuel and can be also used in the food industry, chemical industry and the pharmaceutical industry. The polypeptide protein feed produced by fermenting with the Aspergillus awamori 1042-2 can be used as the additive of the protein of various feed.

Description

Technical field [0001] The present invention relates to the field of microbial products, and more specifically to a strain of Aspergillus awamori with high cellulase production, and also relates to a method for preparing cellulase and polypeptide protein feed by fermentation of the strain of Aspergillus awamori. The strain is suitable for the degradation of cellulose of agricultural and forestry plants to produce fuel ethanol, biological feed, biological fertilizer, food, environmental treatment, textile and clothing, chemical and pharmaceutical industries. Background technique [0002] Cellulase is an enzyme that can play a catalytic role in breaking down cellulose. It is widely present in organisms such as fungi, bacteria and animals. At present, the main application of fungal fermentation to produce cellulase, the strains used are Trichoderma, Aspergillus, and Penicillium. Cellulase is difficult to purify. In practical applications, it is mostly mixed enzymes. It is mainly ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42A23K1/00C12R1/665A23K10/10A23K40/00
Inventor 殷腊生张麒麟
Owner 武汉博普奥多肽生产技术有限公司
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