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Detection method for acute biological toxicity of drinking water contained in plastic bottle

A biotoxicity and detection method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism measurement/inspection, etc., can solve the problem of changing the physical and chemical characteristics of samples, affecting the accuracy of testing, and the rapid safety of bottled water that has not yet been seen. detection and other problems to achieve the effect of ensuring correctness and fast detection speed

Inactive Publication Date: 2010-04-28
EAST CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the detection method of luminescent bacteria toxicity has been widely used in the judgment of water pollution and biological toxicity, and has attracted attention due to its simplicity and low detection cost. T15441-1995; however, if marine photobacteria are used for detection, sodium chloride needs to be added to the sample to reach a final concentration of 3%. Adding such a high concentration of sodium chloride may change the physical and chemical properties of the sample and affect the accuracy of the test
But there is no rapid safety test for drinking bottled water

Method used

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  • Detection method for acute biological toxicity of drinking water contained in plastic bottle
  • Detection method for acute biological toxicity of drinking water contained in plastic bottle
  • Detection method for acute biological toxicity of drinking water contained in plastic bottle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of bacteria solution for test

[0026] 1. Preparation of fresh bacteria solution:

[0027] Use the slant bacteria of the Qinghai Vibrio Q67 strain mentioned above, transfer it to a fresh slant, and incubate at 15-25°C for 12h-18h. At this time, the bacteria glow brightly. Use 10ml of 0.85% NaCl solution to gently wash the bacterial lawn from the slant. , shake well, adjust the concentration of the bacterial solution with a small amount of normal saline to make the concentration of the bacterial solution OD 660 ≈0.3 or so, take 0.1ml of bacterial liquid, add it to a sample test tube with 2ml of normal saline, use a photometer to detect the luminescence, when the luminous intensity is 2-5 million photons / second, this fresh bacterial liquid can be used as a test Bacteria used.

[0028] 2. Preparation of freeze-dried powder bacterial solution:

[0029] Add 5ml resuscitation solution to the freeze-dried powder of Vibrio Qinghai Q67 strain, mix well, and pla...

Embodiment 2

[0042] Bottled distilled water water quality toxicity testing:

[0043] 1. Sampling

[0044] (1) Take distilled water samples directly: take an appropriate amount of bottled distilled water, and add NaCl to a final concentration of 0.8%.

[0045] (2) For the control of the blank water sample, the sub-boiling point distilled water that was double-distilled was used, and NaCl was added to the final concentration of 0.8%.

[0046] 2. Luminescence detection

[0047] The BHP95114 water quality toxicity analyzer produced by Beijing Hamamatsu Photon Technology Co., Ltd. was used as the test instrument.

[0048] You can use freeze-dried powder bacterial liquid, you can also use fresh bacterial liquid.

[0049] Prepare three test small cups, add 2ml of distilled water samples to each small cup, set up three parallel samples, and take three test small cups for blank water sample control, add 2ml each, and set up three parallel samples, each Add 0.1ml of fresh bacterial solution or 0...

Embodiment 3

[0055] Inspection of bottled natural mineral water

[0056] 1. Sampling

[0057] (1) Take mineral water directly to prepare the sample solution: use an appropriate amount of bottled natural mineral water, and add NaCl to a final concentration of 0.8%.

[0058] (2) Preparation of blank control sample solution: use sub-boiling point distilled water re-distilled twice, and add NaCl to a final concentration of 0.8%.

[0059] 2. Luminescence detection

[0060] Prepare three small test cups, add 2ml of mineral water sample solution to each small cup, and set up three parallel samples; take three test small cups for the control sample solution, add 2ml each, and set three parallel samples in turn. Add 0.1ml of fresh bacterial solution or 0.05ml of freeze-dried powder bacterial solution as the test bacterial solution. The time interval for adding bacterial solution to each test small cup is 15s. Luminous Intensity, to calculate the relative luminous intensity.

[0061] 3. Calculat...

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Abstract

The invention relates to a detection method for the acute biological toxicity of drinking water contained in a plastic bottle, belonging to the technical field of drinking water detection. The method comprises the following steps: preparing a bacterial liquid used in tests from a fresh bacterial liquid or a bacterial liquid of freeze-dried powder, preparing a drinking water sample liquid, a control sample liquid, and a detection sample liquid for a plastic bottle, preparing a set of sample parallel samples by using the drinking water sample liquid or the detection sample liquid for the plastic bottle, then preparing a set of control parallel samples, adding 0.1 ml of fresh bacterial liquid or 0.05 ml of bacterial liquid of freeze-dried powder to each set of parallel samples, respectively placing for 15 min, determining the illumination intensity of one sample in each small test cup by a luminescence detecting device, and then calculating the relative illumination intensity. Compared with the prior art, the invention uses a freshwater luminescent bacterium peculiar to China, Vibrio-qinghaiensissp, as a detection strain, and the luminescent bacteria can grow and give out light without sodium chlorides. The invention has the advantages that the physical and chemical characteristics of the samples can not be changed, the correctness of the tests is ensured, and the detection speed is high.

Description

[technical field] [0001] The invention relates to the technical field of drinking water detection, in particular to a method for detecting acute biological toxicity of plastic bottled drinking water. [Background technique] [0002] People need to drink water every day, and the safety of drinking water cannot be ignored. At present, the sales volume of bottled drinking water is very large, and there are many varieties, including pure water, distilled water, natural mineral water or artificial mineralized water. Most of them use plastic bottles as containers. For these commercial drinking water, the country has "Bottle ( Barrel) Hygienic Standards for Drinking Water" GB 19298-2003 standard, which stipulates some main testing indicators, including sensory, physical and chemical and microbial indicators, such testing indicators basically do not involve the evaluation of biological toxicity. As far as the current situation is concerned, the lack of biological toxicity detection ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02G01N21/76C12R1/63
Inventor 朱文杰徐亚同张亚旦
Owner EAST CHINA NORMAL UNIVERSITY
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