Method for separating cephalotaxus fortunei total alkaloids by ion exchange resin

Abstract

The invention discloses a method for separating traditional Chinese medicine cephalotaxus fortunei total alkaloids, in particular to a method for separating total alkaloids from traditional Chinese medicine cephalotaxus fortunei extract, belonging to the traditional Chinese medicine field. The method includes the following technical scheme: cephalotaxus fortunei extract is taken, pH value is adjusted to be 1-7, filtering is carried out, filtrate is taken and added onto cation exchange resin, eluting by water is firstly carried out to remove impurities, and then 0.5-20% saline solution is used for socking the resin, and then 0.5-20% saline solution containing acid or alcohol is used for eluting until no alkaloid is detected, eluant is collected and added with alkali to be natural, and desalinization treatment and concentrated drying are carried out, thus obtaining the traditional Chinese medicine cephalotaxus fortunei total alkaloids. The method overcomes the defects of low extraction rate, high organic solvent dosage, excessive high acid-base concentration, complex technology and impossible for large production and the like of the prior art and can efficiently separate high purity cephalotaxus fortunei total alkaloids in traditional Chinese medicine cephalotaxus fortunei extract.

Description

technical field [0001] The invention relates to a method for extracting and separating active ingredients of traditional Chinese medicines, in particular to a method for extracting and separating total alkaloids of Chinese medicinal cloverleaf from the extract solution of the traditional Chinese medicine cloverleaf, belonging to the field of traditional Chinese medicines. Background technique [0002] Traditional Chinese medicine alkaloids are an important class of substances existing in nature, and more than 700 species have been isolated so far. They are the largest class of active ingredients in traditional Chinese medicine with definite curative effects and a wide range of uses, especially for cancer, liver disease, cardiovascular and cerebrovascular diseases, and rheumatism. etc. have unique curative effects, such as berberine in coptis for antibacterial and anti-inflammatory, ephedrine in ephedra for asthma, reserpine in Rauwolfia for lowering blood pressure, galantamin...

AI Technical Summary

Problems solved by technology

But for the refinement of alkaloids, the biggest deficiency of this method is: firstly, it is difficult to adsorb, and the sample liquid must be a dilute solution, and the alkalinity should be suitable: the alkaloids are still in an ionized state when the alkalinity is low, and the resin The adsorption rate is low; the alkalinity is high, the alkaloids are free, and the macromolecular alkaloids are poorly soluble and precipitated, but still cannot be loaded on the column

However, the steps of the above-mentioned method are cumbersome, and it is difficult to realize industrially processes such as organic solvent extraction or organic solvent reflux extraction of the resin, and the efficiency of eluting alkaloids with lye is very low

At present, this method only stays at the level of preparing small samples in the laboratory, and cannot achieve large-scale production.

[0009] In summary, the above methods generally have defects such as high cost, large consumption of organic solvents, poor selectivity, low purity of total alkaloids, and inability to realize large-scale production. Therefore, purification technology is still the "bottleneck" for the separation of total alkaloids from traditional Chinese medicine. question

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