Testing method of lactate content in zinc lactate

Abstract

The invention discloses a testing method of lactate content in zinc lactate, comprising the following steps of a testing experiment for a zinc lactate sample and a corresponding blank experiment and calculating the lactate content according to the volume difference of titration solutions consumed in the two experiments as well as other data. The method is characterized in that the testing experiment for testing the zinc lactate sample comprises the following flows: taking a zinc lactate sample; adding a standard potassium dichromate solution and a sulphuric acid solution; heating to 80 DEG C and keeping a constant temperature; cooling to a room temperature; diluting; adding potassium iodide; sealing and arranging in a dark place; diluting; titrating with a standard titrating solution of sodium thiosulfate till the diluted solution turn to yellow; adding a starch indicator solution; and finally continuously titrating with the standard titrating solution of sodium thiosulfate until blue color is disappear and bright green color appears and keeps unchanged for 5 minutes. The invention has accurate and reliable testing result, good reproducibility, obvious endpoint coloration, simple operation and high promotion rate and popularization rate and has the action of preventing organic zinc adulteration.

Description

technical field [0001] The invention relates to a method for testing the content of lactate in zinc lactate, in particular to a method for testing the content of lactate in zinc lactate by redox. Background technique [0002] Zinc is an essential trace element for animals. As a component and cofactor of enzymes, it is related to the activities of more than 200 enzymes in animals, regulates and controls the structure and function of these enzymes, and affects various metabolic activities of the body. It has been proved that trace elements play a role in the animal body in the form of organic matter or chelate, rather than in the state of ions. Organic zinc has special physiological functions, higher bioavailability and good biochemical stability, which can improve the growth performance and reproductive performance of animals, reduce the damage of divalent zinc ions to vitamins, and reduce the emission of zinc ions. It is beneficial to environmental protection; at the same t...

AI Technical Summary

Problems solved by technology

Because in the compound zinc lactate, lactate is used as an organic nutrient, its content determines the quality of zinc lactate, if only the method of testing the zinc content to calculate the zinc lactate content is used, it may be harmful to the zinc lactate mixed with other organic zinc. The product gives wrong results, so the content of zinc lactate cannot be accurately measured, so the determination of lactate is also very important

[0004] Zinc lactate is a new product promulgated by the Ministry of Agriculture. There are no national standards or industry standards for its measurement method in feed use at this stage, and there are only some local standards (such as DB13 / 92-91). Calculate the content of zinc lactate, so it will not be discussed here; in addition, there is also a test method for directly testing the content of zinc lactate by ultraviolet spectrophotometry (UV method for short), mainly based on the characteristic that zinc lactate has a maximum absorbance at a wavelength of 209nm in the ultraviolet region The disadvantage is that zinc lactate only absorbs at the ultraviolet edge, the absorption value is unstable, and the error of the test result is relatively large; in addition, the absorption wavelength of other parts of organic zinc is relatively close to that of zinc lactate, so it is impossible to solve the problem of using other organic zinc to replace lactic acid. Zinc adulteration problem

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