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Culture method of black fungus rich in seabuckthorn flavonoids and culture medium thereof

A technology of seabuckthorn flavonoids and cultivation methods, which is applied in the field of cultivation of black fungus rich in seabuckthorn flavonoids and its cultivation medium, can solve the problems of slow mycelial growth and low content of total flavonoids in black fungus, and achieve fast mycelial growth Effect

Inactive Publication Date: 2010-02-10
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problems of slow growth of mycelia and low content of total flavonoids in black fungus in the existing cultivation method, and provides a cultivation method of black fungus rich in flavonoids of seabuckthorn and its cultivation medium

Method used

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specific Embodiment approach 1

[0010] Specific embodiment one: the method for cultivating black fungus rich in seabuckthorn flavonoids in this embodiment is as follows: one, take by mass percentage 64% of hard miscellaneous sawdust, 20% of seabuckthorn slag, 15% of wheat bran, 0.5% of lime and 0.5% gypsum, mixed evenly, to obtain the cultivation medium; 2. Put the cultivation medium into a cultivation bag of 17×33 cm, seal it with a cotton plug, and then sterilize it under the condition of 121° C. 2.0h, or sterilize at 100°C for 8-10h under normal pressure and moist heat, and cool naturally; Cultivate until the black fungus black 29 is covered with the cultivation medium, then use 0.5% potassium permanganate solution to disinfect the outside of the cultivation bag, then cut the mouth, then carry out germination and ear management, until the black fungus fruiting body grows to Eight mature, harvested, and dried to get black fungus rich in seabuckthorn flavonoids.

specific Embodiment approach 2

[0011] Embodiment 2: This embodiment differs from Embodiment 1 in that the high-pressure moist heat sterilization time in step 2 is 1.8 hours. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0012] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 or 2 is that in step 2, the sterilization time under normal pressure is 9 hours. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention discloses a culture method of black fungus rich in seabuckthorn flavonoids and a culture medium thereof and relates to the culture method of the black fungus and the culture medium thereof. The culture method can solve the problems that the hyphae growth speed during the culture process by using the existing culture method is slow, and the content of total flavonoids of the obtainedblack fungus is low. The method is as follows: 1, preparing the culture medium; 2, carrying out high-pressure or normal-pressure damp heat sterilization after bagging and sealing the culture medium, and then carrying out natural cooling; and 3, inoculating a black 29 secondary strain into a bacterial bag after cooling, then disinfecting the bacterial bag, cutting an opening, carrying out centralized germination and growing management, harvesting till a fruiting body of the black fungus grows to 80% of mature and drying in the air, thereby obtaining the black fungus rich in seabuckthorn flavonoids. The obtained black fungus rich in seabuckthorn flavonoids can effectively treat and prevent hypertension and hyperglycemia, suppress platelet aggregation and heart failure and increase the healthcare effect of the black fungus. The hyphae growth speed during the culture process is fast. The obtained product has high yield.

Description

technical field [0001] The invention relates to a method for cultivating black fungus and a culture medium thereof. Background technique [0002] As we all know, black fungus is not only delicious, but also rich in nutrients, high iron content, strong adsorption of colloid, phospholipids (to help delay memory loss), rich in cellulose and animal It is comparable to sexual food and is known as "meat among vegetarians". However, the existing cultivation method has the disadvantages that the growth rate of mycelia is slow during the cultivation process, and the total flavonoid content of the obtained black fungus is low (0.1-0.5mg / 100g). Contents of the invention [0003] The present invention aims to solve the problems of slow mycelium growth rate and low total flavonoid content of black fungus in the cultivation process existing in the existing cultivation method, and provides a cultivation method of black fungus rich in seabuckthorn flavonoids and a cultivation medium ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 张丕奇孔祥辉张介驰韩增华马庆芳戴肖东刘佳宁
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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