Method and device for simulating water bloom floatation of blue algae

A technology of cyanobacterial blooms and simulation devices, which is applied in the direction of microorganism-based methods, stress-stimulated microbial growth methods, biochemical cleaning devices, etc., can solve problems affecting the research progress of the occurrence mechanism of cyanobacteria blooms, achieve low cost, reduce interference, small size effect

Inactive Publication Date: 2010-02-03
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above studies show that researchers at home and abroad have conducted in-depth research on the mechanism of cyanobacterial blooms, but so far, the research on the key stage of cyanobacterial blooms-the floating stage has not been developed at home and abroad, which affects the cyanobacteria bloom. Research Progress on Mechanism of Water Bloom

Method used

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  • Method and device for simulating water bloom floatation of blue algae
  • Method and device for simulating water bloom floatation of blue algae
  • Method and device for simulating water bloom floatation of blue algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] indoor test

[0048] Devices used:

[0049] The device for simulating the floating of cyanobacteria blooms includes air intake pump 1, sterile filter 2, air flow meter 3, valve 4, sealing film of sterile culture container 7, sterile aeration inlet 8, culture solution inlet 9, sterile stirring air flow Inlet 10, reaction column 12, sampling port 13, lift pump 15, culture solution storage tank 16 and light incubator. Because light intensity and temperature can be controlled by using the light incubator, light intensity controller 5, light source 6, circulating water inlet 14, outlet 11, exhaust valve 17, temperature controller 18, temperature probe 19, heating water tank 20 are in this embodiment Do not set. The outer surface of the reaction column 12 is 4cm and 8cm away from the upper part, respectively provided with a sterile aeration inlet 8 and a sterile stirring airflow inlet 10, and the distance between the sampling port 13 and the bottom is 21cm, 29cm, 37cm, 45cm...

Embodiment 2

[0073] Indoor pilot test

[0074] Devices used:

[0075] The device for simulating the floating of cyanobacteria blooms includes air intake pump 1, sterile filter 2, air flow meter 3, valve 4, light intensity controller 5, xenon lamp 6, sealing film of sterile culture container 7, sterile aeration inlet 8, Culture solution inlet 9, sterile stirring air inlet 10, circulating water outlet 11, reaction column 12, sampling port 13, circulating water inlet 14, lift pump 15, culture solution storage tank 16, exhaust valve 17, temperature controller 18, Temperature probe 19, heating tank 20. The specification of the reaction column is 50cm in diameter and 2.2m in height, sampling ports 13 are set every 50cm, and the material of the reaction column is plexiglass. It is characterized in that the top of the reaction column 12 is a transparent sterile sealed structure, and the outer surface of the reaction column 12 is provided with a sterile aeration inlet 8, a culture solution inlet ...

Embodiment 3

[0088] field test

[0089] Devices used:

[0090] The device for simulating the floating of cyanobacteria blooms includes air intake pump 1, sterile filter 2, air flow meter 3, valve 4, sealing film of sterile culture container 7, sterile aeration inlet 8, culture solution inlet 9, sterile stirring air flow Inlet 10, reaction column 12, sampling port 13, lift pump 15, culture solution storage tank 16. The specifications of the reaction column are 50cm in diameter and 2.2m in height. Sampling ports 13 are set every 50cm. The material of the reaction column is plexiglass. Because it is a pilot test in the field, light intensity controller 5, light source 6 and heating device are not provided. It is characterized in that the top of the reaction column 12 is a transparent sterile sealed structure, and the outer surface of the reaction column 12 is sequentially provided with a sterile aeration inlet 8, a culture solution inlet 9, and a sterile stirring air inlet 10 from the top to...

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Abstract

The invention provides a method and a device for simulating the water bloom floatation of blue algae, belonging to the field of water environment. The device comprises a reaction column, a light source and a heating sink, wherein the light source and the heating sink are arranged at the top of the reaction column; the top of the reaction column is sealed aseptically; the outer surface of the reaction column is provided with an aseptic aeration inlet, a culture liquid inlet, an aseptic stirring airflow inlet and a circulating water inlet; the middle of the reaction column is provided with a sampling inlet; and the lower part of the reaction column is provided with a circulating water inlet. The method comprises the following steps: carrying out the amplification culture of algae species; sterilizing culture fluid and a simulating device; placing the culture fluid in the reaction column; inoculating the blue algae into the reaction column for sealing; setting environmental conditions; and then, periodically sampling to analyze and observe the floating characteristics of the blue algae. The method enables the algae to grow in aseptic environments and can effectively simulate the waterbloom of the blue algae. The device has simple structure, small size, easy operation, strong removability, indoor and outdoor use, corollary use with an illumination culture box, convenient manufacture and low cost.

Description

technical field [0001] The invention relates to a method and a device for simulating the occurrence of water blooms, more specifically a method and a device for simulating the floating of cyanobacteria blooms. Background technique [0002] With the rapid development of my country's economy and the acceleration of urban and rural construction, the discharge of pollutants such as nitrogen, phosphorus, and organic matter in areas along rivers and lakes has increased year by year, and the pollution of lake water environment has become increasingly serious, mainly manifested in eutrophication of lake water and cyanobacteria. At present, most of the five major freshwater lakes and urban lakes in the middle and lower reaches of the Yangtze River in my country have entered the stage of eutrophication, and some lake areas of Taihu Lake and Chaohu Lake have entered the stage of heavy eutrophication. [0003] Eutrophication of water body means that lakes and other water bodies receive e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/38C12M1/36C12M1/06C12M1/02C12N1/12C12R1/89
CPCC12M23/06C12M31/02C12M21/02
Inventor 李正魁吴凯石鲁娜陈祈春王月明冯露露
Owner NANJING UNIV
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