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Total flavonoids of broussonetia papyrifera extract and preparation method and application thereof

A technology for the leaves and total flavonoids, which is applied in the field of leaf extracts and its preparation, can solve the problems of total flavonoids of leaves without antibacterial and anti-inflammatory effects, and achieve the effect of simple and practical method and high purity

Inactive Publication Date: 2010-02-03
大连中植环境生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant research report on the total flavonoids of S.

Method used

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  • Total flavonoids of broussonetia papyrifera extract and preparation method and application thereof
  • Total flavonoids of broussonetia papyrifera extract and preparation method and application thereof
  • Total flavonoids of broussonetia papyrifera extract and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, prepare the total flavonoids of mulberry leaves

[0029] Preparation of total flavonoids from the leaves of Structur alba by solvent extraction and solvent extraction:

[0030] 30 mesh leaves (containing petiole) powder 1kg, reflux extraction 2 times with 60% ethanol aqueous solution, extract 2 hours each time with the above-mentioned 60% ethanol aqueous solution of 10 times volume, combine extracts, reclaim ethanol solution , concentrated to a small volume (no alcohol smell), first defatted with half the volume of petroleum ether in the concentrated solution, and extracted until nearly colorless. Then extract three times with an equal amount of n-butanol or ethyl acetate saturated with water, combine the extracts, concentrate under reduced pressure to recover n-butanol or ethyl acetate to obtain an extract; dry the extract under reduced pressure to obtain a total flavonoid extract.

[0031] The extract obtained by n-butanol extraction is 125g, and the re...

Embodiment 2

[0032] Embodiment 2, prepare the total flavonoids of leaves

[0033] Preparation of total flavonoids from the leaves of Structur alba by solvent extraction and polyamide column chromatography:

[0034] The number of meshes is 1kg of leaf powder of 45 meshes, reflux extraction 2 times with 60% ethanol aqueous solution, extract 2 hours each time with the above-mentioned 60% ethanol aqueous solution of 10 times of volume, combine extraction liquid, reclaim ethanol solution, concentrate to small volume, degrease with half the volume of petroleum ether of the small volume concentrate, and extract until nearly colorless. After degreasing, the solution is separated by a polyamide chromatographic column pretreated according to conventional methods, and then eluted with water and 95% ethanol aqueous solution in sequence, and impurities are removed by water elution, and the eluent of 95% ethanol aqueous solution is collected and recovered under reduced pressure. Ethanol, dried to obtai...

Embodiment 3

[0035] Embodiment 3, prepare the total flavonoids of leaves

[0036] Solvent extraction method and resin adsorption method to prepare total flavonoids from the leaves:

[0037] The number of mesh is 1kg of leaf powder of 60 meshes, and the volume percentage of ethanol is 60% ethanol aqueous solution reflux extraction 2 times, each time extracts 2 hours with the above-mentioned 60% ethanol aqueous solution of 10 times of volume, merges extract, reclaims The ethanol solution is concentrated to a small volume, and the degreasing solution is obtained after extraction with half the amount of petroleum ether of the small volume concentrate. The degreasing solution is eluted with water, 65% ethanol aqueous solution, and 95% ethanol aqueous solution successively through the D101 macroporous adsorption resin column that has been pretreated, and the 65% ethanol eluent is decompressed to recover ethanol, and dried to obtain total flavonoids. 105g, the total flavonoids measured are 51g, ...

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Abstract

The invention discloses total flavonoids of broussonetia papyrifera extract and a preparation method and an application thereof. The method comprises the following steps: 1) smashing the broussonetiapapyrifera to powder; 2) extracting to the broussonetia papyrifera powder with ethanol water solution, combining the filter liquor after filtering the extracting solution, concentrating to obtain theconcentrated solution, and degreasing the concentrated solution with the degreasing solvent to obtain degreasing concentrated liquid; and 3) separating and purifying the degreasing concentrated liquidand drying to obtain the total flavonoids of broussonetia papyrifera extract. In the total flavonoids of broussonetia papyrifera extract prepared by the method, the weight content of the total flavonoids is 20%-70% and the yield is 1%-30%. Pharmacologic test proves that the total flavonoids of broussonetia papyrifera extract can be used as bulk pharmaceutical chemicals separately or in combination with other medicines to be applied in the preparation with anti-bacteria and anti-inflammotary drugs. The products prepared by the invention have high purity, and the method is simple and practical,thus the method is suitable for industrialized production.

Description

technical field [0001] The present invention relates to an extract of mulberry leaf and its preparation method and application, in particular to a total flavonoid extract of mulberry leaf and its preparation method and application. Background technique [0002] Broussonetia papyrifera (L.) Ven.t is a plant of the genus Broussonetia papyrifera, which is distributed in most parts of my country, and also in Japan, Vietnam, India and other countries, and its resources are very rich. The ripe fruit of the mulberry tree is called "Chushizi", which is recorded in the "Chinese Pharmacopoeia" in previous editions. It is a common Chinese medicine, which has the effects of nourishing the kidney, clearing the liver, improving eyesight and diuresis. The leaves of the mulberry tree are also known as mulberry leaves, which are cool in nature, sweet in taste, non-toxic, can clear away heat and dampness, cool blood and stop bleeding, kill insects and detoxify, and can be used to treat hemate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/60A61P31/04A61P29/00A61K127/00
Inventor 熊伟窦德强
Owner 大连中植环境生物科技有限公司
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