Method for extracting mulberry leaf gamma-aminobutyric acid and application thereof

An aminobutyric acid and extraction method technology, applied in the field of chemistry, can solve problems such as unfavorable large-scale application production, destruction of γ-aminobutyric acid, cumbersome extraction process, etc., and achieve the effects of low production cost, easy extraction, and mature process conditions.

Inactive Publication Date: 2012-12-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology requires time-consuming and energy-consuming pre-drying treatment steps for mulberry leaves, and the extraction process is also cumbersome. Heating is required for soaking, which is easy to damage γ-aminobutyric acid, high energy consumption, low yield, and the obtained γ-aminobutyric acid Butyric acid is a mixture, the purity can only reach about 70%, it is not suitable for food additives or pharmaceutical raw materials for medical and health use, and it is not conducive to large-scale application and production

Method used

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  • Method for extracting mulberry leaf gamma-aminobutyric acid and application thereof
  • Method for extracting mulberry leaf gamma-aminobutyric acid and application thereof
  • Method for extracting mulberry leaf gamma-aminobutyric acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Extraction and preparation of γ-aminobutyric acid

[0056] 1. The specific operation steps are as follows:

[0057] (1) mulberry leaves are dried and pulverized;

[0058] Mature leaves of the mulberry variety Kangqing 10 were picked, rinsed with clean water to remove the dust attached to the surface of the mulberry leaves, dried in the air, baked in an oven at 85°C for 30 minutes, and then cooled to 65°C for 4.2 hours.

[0059] The dried samples were pulverized, sealed in bags and stored at low temperature (5-0°C) for later use.

[0060] (2) prepare the crude extract of gamma-aminobutyric acid

[0061] Add water to dry mulberry leaf powder according to the ratio of mulberry leaf powder weight (g): water volume (mL) of 1:30, soak for 15min, then carry out ultrasonic-microwave synergistic extraction under the conditions of microwave 144W and ultrasonic wave 50W, time After 160s, the extract was centrifuged to remove the precipitate, and the supernatant was collected an...

Embodiment 2

[0074] Extraction and preparation of γ-aminobutyric acid

[0075] (1) mulberry leaves are dried and pulverized;

[0076] Mature leaves of the mulberry variety Kangqing 10 were picked, rinsed with clean water to remove the dust attached to the surface of the mulberry leaves, dried in the air, baked in an oven at 83°C for 32 minutes, and then cooled to 67°C for 4 hours.

[0077] The dried samples were pulverized, sealed in bags and stored at low temperature (5-0°C) for later use.

[0078] (2) prepare the crude extract of gamma-aminobutyric acid

[0079] Add water to the dry mulberry leaf powder according to the ratio of mulberry leaf powder weight (g): water volume (mL) of 1:35, soak for 13 minutes, and then carry out ultrasonic-microwave cooperative extraction under the conditions of microwave 144W and ultrasonic wave 50W. After 170 s, the extract was centrifuged to remove the precipitate, and the supernatant was collected and concentrated by rotary evaporation.

[0080] (3)...

Embodiment 3

[0089] Example 3 Extraction and preparation of γ-aminobutyric acid

[0090] (1) mulberry leaves are dried and pulverized;

[0091] Mature leaves of the mulberry variety Kangqing 10 were picked, rinsed with clean water to remove the dust attached to the surface of the mulberry leaves, dried in the air, baked in an oven at 87°C for 28 minutes, and then cooled to 63°C for 4.5 hours. The dried samples were pulverized, sealed in bags and stored at low temperature (5-0°C) for future use.

[0092] (2) prepare the crude extract of gamma-aminobutyric acid

[0093] Add water to the dry mulberry leaf powder according to the ratio of mulberry leaf powder weight (g): water volume (mL) of 1:32, soak for 17 minutes, and then carry out ultrasonic-microwave synergistic extraction under the conditions of microwave 144W and ultrasonic wave 50W. After 165 s, the extract was centrifuged to remove the precipitate, and the supernatant was collected and concentrated by rotary evaporation.

[0094]...

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Abstract

The invention discloses a method for extracting mulberry leaf gamma-aminobutyric acid and an application thereof. In the invention, mulberry leaves are utilized as a raw material, a coarse extract is prepared by mulberry leaf drying, pulverization, water leaching and ultrasonic wave-microwave synergistic extraction, and then a gamma-aminobutyric acid white crystal with high purity is prepared by three-stage separation and purification after parts of doped proteins and polysaccharide substances of the coarse extract are removed. The invention provides the active functions of the mulberry leaf gamma-aminobutyric acid for promoting the growth and development of animals and reinforcing the anti-fatigue capability. The invention has simple method and mature technological conditions, is suitable for industrialized production, provides a technical base for further developing a novel application of the mulberry leaves in the non-silk industry and puts forward a way of applying the mulberry leaves GABA to the fields of the medicine, health-care foods, the livestock industry and the like so as to enable the mulberry leaves GABA to become a novel luminous point for extending and developing the silk road.

Description

technical field [0001] The invention belongs to the technical field of chemistry, and in particular relates to an extraction method and application of gamma-aminobutyric acid, a bioactive substance of mulberry leaves. Background technique [0002] Gamma-aminobutyric acid (GABA) is a functional non-protein amino acid. Since Stewatd et al. (1949) first discovered in potato stems, Roberts and Udenfriend also found the existence of GABA in mammalian brains in 1950. Subsequent studies have shown that GABA is widely distributed in animals, plants and microorganisms. It exists in both and is an inhibitory transmitter in the nervous center of mammals. It has important physiological functions, such as regulating blood pressure, promoting mental stability, promoting blood flow in the brain, increasing brain vitality, nourishing nerve cells, strengthening the liver and kidney, etc. Multiple effects. Clinical medical research believes that GABA has an important active function in impr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C229/08C07C227/28A61K31/197A61P3/02A61P43/00A23L1/305A23L33/175
Inventor 林健荣陈恒文钟杨生王叶元廖富蘋蓝年新
Owner SOUTH CHINA AGRI UNIV
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