Method and equipment for inactivating cryptosporidium and giardia cysts inside water
A technology for cryptosporidium and giardia, which is applied to the field of inactivating cryptosporidium oocysts and giardia cysts in water bodies, can solve the problems of low inactivation rate, secondary pollution, complicated equipment, etc., and achieves easy popularization and application. , Easy to operate control, easy to operate effect
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example 1
[0037] In this embodiment, the above-mentioned method and equipment are used to carry out photocatalytic deactivation of Cryptosporidium oocysts and Giardia cysts in water, and the specific steps are as follows:
[0038] (1) Provide the water to be treated, and carry out the purification and collection of the two worms: after the samples of the water body to be treated are collected, they are respectively filtered using a 60-150 mesh screen, floated with saturated zinc sulfate, and centrifuged with 1:1 and 1:2 sucrose gradients (rotating speed) 12000r / min), rinsed three times with phosphate buffer solution to obtain a concentration of 1.5×10 6 samples / mL of two worms, suspended in 2.5% potassium dichromate, and finally stored in a refrigerator at 4°C;
[0039] (2) Preparation of photocatalytic reagent: the rutile TiO 2 and FeC 2 o 4 The solution was mixed and stirred according to the aforementioned 10:1 ratio and adsorbed for 48 hours, then the water was evaporated to dryne...
example 2
[0047] According to the method described in Example 1, pure water was used as a water sample for a comparative test. Under the same irradiation time, take 10 minutes and set different light intensities Lightoutput (mw / cm 2 ), irradiate the experimental sample, and identify the inactivation rate of Giardia by the method of example one. The capacity of each reaction treatment solution was 50mL, and the reaction solution was stirred during the experiment to prevent the catalyst from settling. Other steps and parameters are the same as Example 1. Irradiation intensity: 2, 3.5, 4.5, 8, 10, 13, 25mw / cm 2 . Experimental results refer to figure 2 Marked as UV / TiO 2 The curve shows that under the same irradiation time, the water body is irradiated with ultraviolet light for inactivation treatment with photocatalytic reagents added, and the irradiation light intensity Lightoutput (mw / cm 2 ) on the inactivation race (%) of Giardia inactivation rate.
example 3
[0049] According to the method of Example 1, pure water was used as a water sample for a comparative test. Under the same light intensity, the irradiation intensity was 5mw / cm 2 , respectively set different irradiation time lengths, irradiate water samples, and identify the inactivation rate of Cryptosporidium according to the method of Example 1. The capacity of each reaction treatment solution was 50mL, and the reaction solution was stirred during the experiment to prevent the catalyst from settling. Other steps and parameters are the same as Example 1. The irradiation time is: 0, 5, 10, 15, 20, 25, 30, 60 minutes, the experimental results refer to image 3 Marked as UV / TiO 2 The curve shows that under the same irradiation light intensity, the water sample is irradiated with photocatalytic reagents for inactivation treatment, and the irradiation time t (min) affects the inactivation race (%) of Cryptosporidium inactivation rate influences.
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