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Homocysteine diagnosis kit and method for determining homocysteine concentration

A technology of homocysteine ​​and reagents, which is applied in the field of medical inspection and measurement, and can solve the problems of large variation of test data, complicated operation, and long test time

Inactive Publication Date: 2010-01-06
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but its disadvantages are complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The homocysteine ​​diagnostic reagent of this embodiment is a single reagent, including:

[0044] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0045] Stabilizer 500mmol / L

[0046] Coenzyme 3mmol / L

[0047] Homocysteine-α, γ-lyase 10000U / L

[0048] Guanosine monophosphate reductase 12000U / L

[0049] Inosine monophosphate 10mmol / L

[0050] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0051] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of tested homocysteine ​​sample to reagent is 1 / 25, the reaction direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0052] After adding the samples and reagents, l...

Embodiment 2

[0054] The homocysteine ​​diagnostic reagent of this embodiment is a dual reagent, including:

[0055] Reagent 1

[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Coenzyme 3mmol / L

[0059] Inosine monophosphate 10mmol / L

[0060] Reagent 2

[0061] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 50mmol / L

[0063] Homocysteine-α, γ-lyase 10000U / L

[0064] Guanosine monophosphate reductase 12000U / L

[0065] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0066] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, tested homocysteine ​​sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction is positive reaction (rising reaction), the delay...

Embodiment 3

[0069] The homocysteine ​​diagnostic reagent of this embodiment is three reagents, including:

[0070] Reagent 1

[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0072] Stabilizer 50mmol / L

[0073] Coenzyme 3mmol / L

[0074] Inosine monophosphate 10mmol / L

[0075] Reagent 2

[0076] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0077] Stabilizer 500mmol / L

[0078] Guanosine monophosphate reductase 12000U / L

[0079] Reagent 3

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 500mmol / L

[0082] Homocysteine-α, γ-lyase 10000U / L

[0083] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0084] When measuring the concentration of homocysteine, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 0.1, test main wavelength 340nm, te...

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Abstract

The invention relates to a homocysteine diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining homocysteine concentration, and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, coenzyme, inosine monophosphate, homocysteine-alpha, gamma-lyase, guanosine monophosphate reducase and a stabilizer. The method for determining homocysteine concentration comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending degree of absorbance at the position where the dominant wave length is 340nm to determine the concentration of homocysteine.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnostic kit, and at the same time, the invention also relates to a method for measuring the concentration of homocysteine, which belongs to the technical field of medical examination and determination. Background technique [0002] There are many methods for measuring homocysteine ​​concentration in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, Fluorescence polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] High-performance liquid chromatography (HPLC): It is a classic reference method, but its operation is relatively complicated, the test time is relatively long, and the test data varies greatly. It is gradually adopted by enzyme-linked immunoassay, fluorescence polarization analysis and enzymatically replaced. High-perf...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/25G01N33/68C12Q1/527C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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